FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6153505
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5287428.592533848595231No Hit
CAACGGAATCCCAAAAGCAGCTG4996468.119697635737682No Hit
AACATTCAACGCTGTCGGTGAGT1982603.221903614281617No Hit
AAGCTGCCAGTTGAAGAACTGT1963933.1915631822839177No Hit
CCCCCACTGCTAAATTTGACTG1799672.9246258839474413No Hit
TTCAAGTAATCCAGGATAGGCT1416322.3016475975886914No Hit
AACATTCAACGCTGTCGGTGAG1376192.2364327322395936No Hit
TATTGCACTTGTCCCGGCCTGTAA1061351.7247893680105892No Hit
TAGCTTATCAGACTGATGTTGACT995411.6176309274145386No Hit
TAGCTTATCAGACTGATGTTGAC932621.5155915206049235No Hit
TATTGCACTTGTCCCGGCCTGTAT793421.2893789799472009No Hit
TAGCAGCACGTAAATATTGGCG600200.975379072577336No Hit
TGTAAACATCCTTGACTGGAAGCT564130.9167620729974217No Hit
TGTAAACATCCCCGACTGGAAGCT556730.9047364063245257No Hit
ACTGGACTTGGAGTCAGAAGGC505910.8221493279033657No Hit
TAGCTTATCAGACTGATGTTGA500080.8126750526732327No Hit
CACCCGGCTGTGTGCACATGTGC488100.7932064733838682No Hit
TATTGCACTTGTCCCGGCCTGT485020.7882011959037979No Hit
TATTGCACTTGTCCCGGCCTGTA440580.7159821922627836No Hit
AGCAGCATTGTACAGGGCTATGA375110.609587544009471No Hit
CAACGGAATCCCAAAAGCAGCT330090.5364259881157162No Hit
GGAATACCGGGTGCTGTAGGCTTT323450.5256353899119283No Hit
TGAGGTAGTAGGTTGTATAGTT304240.4944174092651262No Hit
TTAATGCTAATCGTGATAGGGGTT279080.45353014257727914No Hit
CAACGGAATCCCAAAAGCAGCTGT277550.45104375473815334No Hit
GGAATACCGGGTGCTGTAGGCTT240200.3903466398418462No Hit
CAACACCAGTCGATGGGCTGT206540.3356461073810779No Hit
CAAAGAATTCTCCTTTTGGGCT195620.31790012358810144No Hit
TCGTTTCCCGGCCAATGCACCA187840.30525692268065113No Hit
CACTAGATTGTGAGCTCCTGGA178310.28976981411406993No Hit
CACTAGATTGTGAGCTCCTGGAA171670.2789792159102821No Hit
CATAAAGTAGAAAGCACTACT163840.2662547604982851No Hit
TGAGAACTGAATTCCATGGGTT154740.2514664406708047No Hit
TATTGCACTTGTCCCGGCCTGTAG154250.250670146526248No Hit
TCGTACGACTCTTAGCGG147180.2391807595833594No Hit
ACTGGACTTGGAGTCAGAAGGCT147070.23900199967335692No Hit
CCCATAAAGTAGAAAGCACTAC142650.23181910147143783No Hit
CCCATAAAGTAGAAAGCACT142460.23151033435416077No Hit
AACATTCAACGCTGTCGGTGAGTT141930.23064903660596683No Hit
ACTGGACTTGGAGTCAGAAGGCA137130.22284860416949365No Hit
GCCTGTCTGAGCGTCGCT134110.21794083209487924No Hit
CGCCTGTCTGAGCGTCGCT131940.21441438659755702No Hit
TAGCTTATCAGACTGATGTTGACA125720.2043063262319605No Hit
CATTGCACTTGTCTCGGTCTGA124710.20266498524011922No Hit
TTCAAGTAATTCAGGATAGGTT121230.1970096717236762No Hit
TGTAAACATCCCCGACTGGAAGC116730.18969676631448257No Hit
CCCCCACTGCTAAATTTGACTGGCTT114480.18604031360988574No Hit
TGAGGTAGTAGTTTGTGCTGTT114230.18563404108715278No Hit
CAAAGAATTCTCCTTTTGGGCTT113990.1852440194653291No Hit
ACTTGACTTGGAGTCAGAAGGC113590.18459398342895633No Hit
ACCACAGGGTAGAACCACGGAC108980.1771023181097602No Hit
TGAATACCGGGTGCTGTAGGCTTT106760.17349461810789135No Hit
AACGGAATCCCAAAAGCAGCTG104300.16949689648419886No Hit
AATGCACCCGGGCAAGGATTCT97840.15899881449677866No Hit
TGAGGTAGTAGGTTGTGTGGTT96460.1567561901712926No Hit
TATTGCACTTGTCCCGGCCTGTAAA95530.15524485638672594No Hit
TGTAAACATCCTACACTCTCAGCT95410.1550498455758141No Hit
TGAGGTAGTAGATTGTATAGTT95390.15501734377399548No Hit
TGTCAGTTTGTCAAATACCCCAA94680.1538635298094338No Hit
TATTGCACTTGTCCCGGCCTGTT94510.1535872644939754No Hit
ACCCCCCACTGCTAAATTTGACTG91210.14822446719390006No Hit
ACCGGGTGCTGTAGGCTT90430.14695689692297317No Hit
ACTGGACTTGGAGTCAGAAGG90370.14685939151751726No Hit
TGAGAACTGAATTCCATAGGCTGT89670.14572182845386492No Hit
CACCCGTAGAACCGACCTTGCG89460.1453805595347692No Hit
CAAAGTGCTGTTCGTGCAGGTAG89010.14464926899384983No Hit
CAACGGAATCCCAAAAGCAGCTGA88930.14451926178657531No Hit
CCCCCCACTGCTAAATTTGACTGG84880.13793764691830102No Hit
TATTGCACTTGTCCCGGCCTGTAAT83630.13590628430463614No Hit
CTCCCACTGCTTCACTTGACTAG82630.1342811942137042No Hit
TTCACAGTGGCTAAGTTCTG78910.1282358590754375No Hit
AGCTCGGTCTGAGGCCCCTCAGT78210.12709829601178516No Hit
TGAATACCGGGTGCTGTAGGCTT77200.12545695501994392No Hit
TTCACAGTGGCTAAGTTCCG76670.12459565727175001No Hit
ATGCACCTGGGCAAGGATTCTGA75670.12297056718081809No Hit
TAATGTGAATTGCAGGACA75560.12279180727081558No Hit
CCCCCCACTGCTAAATTTGACTGGCT73730.11981789240441017No Hit
TGAGGTAGTAGTTTGTACAGTT70200.1140813243834205No Hit
TCGTTTCCCGGGCGGCGCACCA69610.11312252122977068No Hit
TTCAAGTAATCCAGGATAGGCTT68280.11096115140883123No Hit
CTACGCCTGTCTGAGCGTCGCT67550.10977483564245093No Hit
CTCCCACTGCTTCACTTGACTAGCCT66840.10862102167788926No Hit
ACCGGGTGCTGTAGGCTTT66570.10818224735333765No Hit
TTTAAACATCCTTGACTGGAAGCT61590.10008929870049671No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)