FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8467127
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7954639.39472149171732No Hit
CCCCCCACTGCTAAATTTGACTG4657465.500637937756219No Hit
AACATTCAACGCTGTCGGTGAGT3970164.688910417902082No Hit
AACATTCAACGCTGTCGGTGAG2725183.218541543075945No Hit
TTCAAGTAATCCAGGATAGGCT2647253.12650324011911No Hit
TAGCAGCACGTAAATATTGGCG2617043.090824077635779No Hit
AAGCTGCCAGTTGAAGAACTGT2369002.7978793751410604No Hit
CCCCCACTGCTAAATTTGACTG1821902.1517334037861957No Hit
TATTGCACTTGTCCCGGCCTGTAA1705462.0142133217087683No Hit
TAGCTTATCAGACTGATGTTGAC1470141.736291424470189No Hit
TATTGCACTTGTCCCGGCCTGTAT1390241.6419264763596908No Hit
TAGCTTATCAGACTGATGTTGACT1380521.6304467855507538No Hit
TGAGGTAGTAGGTTGTATAGTT1338931.5813274089310343No Hit
TAGCTTATCAGACTGATGTTGA1052131.2426056677784565No Hit
TATTGCACTTGTCCCGGCCTGT994411.174436145814277No Hit
TGAGGTAGTAGATTGTATAGTT823730.9728565545314248No Hit
TCGTACGACTCTTAGCGG764200.9025493535174327No Hit
CATAAAGTAGAAAGCACTACT743270.8778302250574487No Hit
TATTGCACTTGTCCCGGCCTGTA724480.8556385182364691No Hit
TGTAAACATCCTTGACTGGAAGCT719970.8503120361841744No Hit
AGCAGCATTGTACAGGGCTATGA601430.7103117739937054No Hit
ACTGGACTTGGAGTCAGAAGGC599690.7082567676143278No Hit
TGTAAACATCCCCGACTGGAAGCT560780.6623025732341088No Hit
TTCAAGTAATTCAGGATAGGTT496730.5866570797863313No Hit
CCCATAAAGTAGAAAGCACTAC427500.5048938087263838No Hit
CACCCGGCTGTGTGCACATGTGC422420.49889413492912055No Hit
CAACGGAATCCCAAAAGCAGCTGT393580.46483299471001205No Hit
CAACGGAATCCCAAAAGCAGCT374810.44266490865201386No Hit
CCCATAAAGTAGAAAGCACT366550.43290953354071576No Hit
CATTGCACTTGTCTCGGTCTGA362050.4275948618699117No Hit
TGAGGTAGTAGTTTGTACAGTT342520.4045291868186222No Hit
AACATTCAACGCTGTCGGTGAGTT314350.3712593421593889No Hit
CGCCTGTCTGAGCGTCGCT296790.35052031226176245No Hit
CAACACCAGTCGATGGGCTGT282230.33332439681133874No Hit
CTCCCACTGCTTCACTTGACTAG274490.3241831615375558No Hit
TGAGGTAGTAGTTTGTGCTGTT246530.2911613348896267No Hit
TGTAAACATCCTACACTCTCAGCT243010.28700408060490884No Hit
TCGTTTCCCGGCCAATGCACCA237330.2802957839182051No Hit
AACGGAATCCCAAAAGCAGCTG234010.27637473726330075No Hit
TGTCAGTTTGTCAAATACCCCAA230240.27192222344131606No Hit
GGAATACCGGGTGCTGTAGGCTTT225470.26628867147026375No Hit
TAGCTTATCAGACTGATGTTGACA212560.2510414689658015No Hit
TATTGCACTTGTCCCGGCCTGTAG203080.23984522731264102No Hit
CAAAGAATTCTCCTTTTGGGCT190930.2254956138014701No Hit
TATTGCACTTGTCCCGGCCTGTT181670.21455920054110444No Hit
TAGCAGCACGTAAATATTGGC175870.20770917927651258No Hit
ACTGGACTTGGAGTCAGAAGGCA175470.20723676401688554No Hit
CACTAGATTGTGAGCTCCTGGA173780.20524080954496135No Hit
GCCTGTCTGAGCGTCGCT169750.2004812258042191No Hit
TTCTCACTACTGCACTTGACTAG168320.19879234125105244No Hit
GGAATACCGGGTGCTGTAGGCTT168280.19874509972508972No Hit
CACTAGATTGTGAGCTCCTGGAA167210.19748138890558747No Hit
ACTGGACTTGGAGTCAGAAGGCT161530.1907730922188837No Hit
CCCCCACTGCTAAATTTGACTGGCTT157950.1865449756452218No Hit
TGAGGTAGTAGGTTGTGTGGTT153860.18171452961553547No Hit
ATCCCGGACGAGCCCCCA151320.17871469271690385No Hit
TGTAAACATCCCCGACTGGAAGC147520.1742267477504471No Hit
TTCACAGTGGCTAAGTTCCG144770.1709788928405113No Hit
TGTCAGTTTGTCAAATACCCCA144160.17025845956958008No Hit
TTCAAGTAATCCAGGATAGGC140440.16586499765504875No Hit
ACTGGACTTGGAGTCAGAAGG140330.16573508345865134No Hit
TTATAAAGCAATGAGACTGATT139030.16419973386486347No Hit
TTCAAGTAATCCAGGATAGGCTT132660.15667652085530312No Hit
CAAAGAATTCTCCTTTTGGGCTT131960.15584979415095582No Hit
CTTCTCACTACTGCACTTGACTAG130320.153912891586485No Hit
TCAGTGCACTACAGAACTTTGT129250.15264918076698272No Hit
CTCCCACTGCTTCACTTGACTAGCCT127210.15023986294288488No Hit
TATTGCACTTGTCCCGGCCTGTAAA127010.15000365531307136No Hit
AGAATTACTCTGAGACCT125610.14835020190437676No Hit
TGAGGTAGTAGGTTGTATAGT123740.14614166056562045No Hit
CAACGGAATCCCAAAAGCAGCTGA119180.14075612660587233No Hit
AACATTCAACGCTGTCGGTGA119080.14063802279096557No Hit
CCCATAAAGTAGAAAGCACTA118320.13974043379767423No Hit
AGCAGCATTGTACAGGGCTATGT118230.13963414036425814No Hit
ACCACAGGGTAGAACCACGGAC117060.1382523257298491No Hit
TTCACAGTGGCTAAGTTCTG113290.13379981190786439No Hit
TATTGCACTTGTCCCGGCCTGTAAT110830.13089445806115815No Hit
ACCCCCCACTGCTAAATTTGACTG110750.13079997500923277No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC108620.12828436375171884No Hit
TTCACAGTGGCTAAGTTCCGC108100.1276702239142037No Hit
TTCACAGTGGCTAAGTTCTGC106670.12598133936103711No Hit
TATTGCACTTGTCCCGGCCTGTTT104870.12385547069271548No Hit
AGCTCGGTCTGAGGCCCCTCAGT102830.12144615286861765No Hit
CCCCCCACTGCTAAATTTGACTGGCT102650.1212335660017855No Hit
TCGTTTCCCGGGCGGCGCACCA102570.12113908294986007No Hit
AGCAGCATTGTACAGGGCTATG100410.11858804054787415No Hit
TACGCCTGTCTGAGCGTCGCT98880.11678105217980078No Hit
CTACGCCTGTCTGAGCGTCGCT98200.11597794623843484No Hit
CAAAGTGCTGTTCGTGCAGGTAG97860.11557639326775185No Hit
AACATTCAACGCTGTCGGTGAGA96590.11407647481843604No Hit
ATGCACCTGGGCAAGGATTCTGA95650.11296629895831253No Hit
AGAGGTAGTAGGTTGCATAGTT94730.11187974386117037No Hit
TATTGCACTTGTCCCGGCCTGA90680.10709653935744674No Hit
AATGCACCCGGGCAAGGATTCT88900.1049942914521065No Hit
TGTAAACATCCTACACTCAGCT87360.10317549270254242No Hit
TAGCAGCACATCATGGTTTACA87050.10280937087633149No Hit
ATCACATTGCCAGGGATTTCC86890.10262040477248068No Hit
TAGCAGCACGTAAATATTGGCGT85590.10108505517869285No Hit
GTCAGGATGGCCGAGCGGTCTAAGG85580.10107324479720217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)