FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8396218
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG148176117.64795768761602No Hit
CAACGGAATCCCAAAAGCAGCTG8178359.74051650397834No Hit
CCCCCACTGCTAAATTTGACTG4454535.305400598221723No Hit
AACATTCAACGCTGTCGGTGAGT2642443.1471788845882753No Hit
TAGCAGCACGTAAATATTGGCG2454252.9230422554535864No Hit
TTCAAGTAATCCAGGATAGGCT2388442.8446617274587203No Hit
AAGCTGCCAGTTGAAGAACTGT2339142.7859448146772747No Hit
AACATTCAACGCTGTCGGTGAG1735242.066692408415313No Hit
TATTGCACTTGTCCCGGCCTGTAA1283901.5291408584198267No Hit
TATTGCACTTGTCCCGGCCTGTAT1181651.407359837488736No Hit
TAGCTTATCAGACTGATGTTGAC915121.0899192946157426No Hit
TAGCTTATCAGACTGATGTTGACT905041.0779138893249318No Hit
TGAGGTAGTAGGTTGTATAGTT879971.0480552077137586No Hit
TATTGCACTTGTCCCGGCCTGT711010.8468217476011224No Hit
TGTAAACATCCTTGACTGGAAGCT650310.7745272931217364No Hit
TAGCTTATCAGACTGATGTTGA603790.7191213948947014No Hit
TCGTACGACTCTTAGCGG588990.7014944109359714No Hit
TATTGCACTTGTCCCGGCCTGTA524750.6249837724556461No Hit
CAACGGAATCCCAAAAGCAGCTGT497670.5927311558608888No Hit
CATAAAGTAGAAAGCACTACT474160.5647304536399602No Hit
TGAGGTAGTAGATTGTATAGTT469800.5595376394467129No Hit
AGCAGCATTGTACAGGGCTATGA467020.5562266248923027No Hit
TTCAAGTAATTCAGGATAGGTT441260.5255461447046753No Hit
TGTAAACATCCCCGACTGGAAGCT409150.4873027355888092No Hit
ACTGGACTTGGAGTCAGAAGGC399810.47617867949593495No Hit
CCCATAAAGTAGAAAGCACTAC343520.4091365898312788No Hit
TTCTCACTACTGCACTTGACTAG324470.3864478030465622No Hit
CTTCTCACTACTGCACTTGACTAG300150.35748238075762206No Hit
CAACGGAATCCCAAAAGCAGCT299120.3562556379550888No Hit
CACCCGGCTGTGTGCACATGTGC295840.35234911718585676No Hit
CATTGCACTTGTCTCGGTCTGA266820.31778593647759024No Hit
CCCATAAAGTAGAAAGCACT238850.28447331882044985No Hit
CCCCCACTGCTAAATTTGACTGGCTT238140.2836276999954027No Hit
CTCCCACTGCTTCACTTGACTAG231080.2752191522421166No Hit
ACCCCCCACTGCTAAATTTGACTG213950.2548171093223163No Hit
CCCCCCACTGCTAAATTTGACTGG213250.25398340062156555No Hit
TGAGGTAGTAGTTTGTACAGTT198730.2366899001431359No Hit
CAAAGAATTCTCCTTTTGGGCT196840.23443888665110887No Hit
CCCCCCACTGCTAAATTTGACTGGCT194670.23185438967878155No Hit
TGTAAACATCCTACACTCTCAGCT187980.22388651652446376No Hit
AACGGAATCCCAAAAGCAGCTG187670.22351730267127415No Hit
AACATTCAACGCTGTCGGTGAGTT187120.22286224583496997No Hit
TAGCAGCACGTAAATATTGGC161920.19284873260794325No Hit
CACTAGATTGTGAGCTCCTGGA160860.1915862594325207No Hit
CACTAGATTGTGAGCTCCTGGAA155320.18498805057229337No Hit
TATTGCACTTGTCCCGGCCTGTAG152230.18130782216469368No Hit
CAACACCAGTCGATGGGCTGT148550.17692489642360407No Hit
TGTCAGTTTGTCAAATACCCCAA144970.17266107192547883No Hit
TAGCTTATCAGACTGATGTTGACA141880.16898084351787912No Hit
TATTGCACTTGTCCCGGCCTGTT140580.16743252735934203No Hit
TGAGGTAGTAGTTTGTGCTGTT137330.16356173696299928No Hit
CAAAGAATTCTCCTTTTGGGCTT137040.16321634335840254No Hit
GCCTGTCTGAGCGTCGCT127330.1516516126665601No Hit
GACCCGCGGGCGCTCTCCAGTCCT122910.14638733772753398No Hit
TTCAAGTAATCCAGGATAGGCTT122710.14614913524160522No Hit
AGCTCGGTCTGAGGCCCCTCAGT122700.14613722511730876No Hit
CTCCCACTGCTTCACTTGACTAGCCT119540.14237362583963398No Hit
TACGCCTGTCTGAGCGTCGCT116590.13886013917218443No Hit
TGTAAACATCCCCGACTGGAAGC116340.13856238606477345No Hit
ACTGGACTTGGAGTCAGAAGGCA109740.13070170402912357No Hit
CGCCTGTCTGAGCGTCGCT108150.12880799426598977No Hit
TATTGCACTTGTCCCGGCCTGTAAT108070.12871271327161826No Hit
CCCCCCACTGCTAAATTTGACTGT108070.12871271327161826No Hit
TGTCAGTTTGTCAAATACCCCA106880.127295408480342No Hit
ACTGGACTTGGAGTCAGAAGGCT105960.12619967704506957No Hit
TCGTTTCCCGGCCAATGCACCA105560.12572327207321202No Hit
TATTGCACTTGTCCCGGCCTGTTT105410.12554462020876542No Hit
TTCAAGTAATCCAGGATAGGC103370.12311495485229182No Hit
AGCAGCATTGTACAGGGCTATGT102030.12151899819656897No Hit
TGATGTAGTAGGTTGTATAGTT100380.11955382768765652No Hit
CCCCCCACTGCTAAATTTGACTGA94770.11287224795735412No Hit
CTACGCCTGTCTGAGCGTCGCT92260.1098828067589479No Hit
TATTGCACTTGTCCCGGCCTGTAAA88560.10547606076926541No Hit
ATCCCGGACGAGCCCCCA88560.10547606076926541No Hit
CGTTCGCGCTTTCCCCTG86730.10329650802301703No Hit
TCGTTTCCCGGGCGGCGCACCA86670.10322504727723839No Hit
TGAGGTAGTAGGTTGTGTGGTT86290.10277246255397371No Hit
CCCATAAAGTAGAAAGCACTA86260.1027367321810844No Hit
TTCACAGTGGCTAAGTTCTGC85990.10241515882508054No Hit
TTCACAGTGGCTAAGTTCCGC85050.10129560714121524No Hit
CCCCCCACTGCTAAATTTGACTGTA84700.10087875279083988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)