FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8059077
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG166763820.69266741092063No Hit
CAACGGAATCCCAAAAGCAGCTG7336459.103337764361848No Hit
CCCCCACTGCTAAATTTGACTG4820365.98128048658674No Hit
AACATTCAACGCTGTCGGTGAGT2977943.6951377930748146No Hit
TTCAAGTAATCCAGGATAGGCT2092122.595979663676126No Hit
TAGCAGCACGTAAATATTGGCG2020622.507259826404438No Hit
AACATTCAACGCTGTCGGTGAG1980272.4571920580979683No Hit
AAGCTGCCAGTTGAAGAACTGT1855952.3029312165648745No Hit
TATTGCACTTGTCCCGGCCTGTAA1383051.7161394536868178No Hit
TATTGCACTTGTCCCGGCCTGTAT1210491.502020640825246No Hit
TAGCTTATCAGACTGATGTTGACT978391.2140223998356139No Hit
TAGCTTATCAGACTGATGTTGAC907911.1265682161865436No Hit
TGAGGTAGTAGGTTGTATAGTT778060.9654455466798493No Hit
TGTAAACATCCTTGACTGGAAGCT706250.876341049973837No Hit
TATTGCACTTGTCCCGGCCTGT560010.6948810639233252No Hit
TATTGCACTTGTCCCGGCCTGTA523770.6499131352138712No Hit
TAGCTTATCAGACTGATGTTGA521790.6474562781817322No Hit
TGTAAACATCCCCGACTGGAAGCT476580.5913580426145575No Hit
ACTGGACTTGGAGTCAGAAGGC461850.5730805152997049No Hit
AGCAGCATTGTACAGGGCTATGA455340.5650026671788841No Hit
TGAGGTAGTAGATTGTATAGTT420320.521548559469031No Hit
CAACGGAATCCCAAAAGCAGCTGT414320.5141035381595187No Hit
CATAAAGTAGAAAGCACTACT385790.47870246183278803No Hit
CCCATAAAGTAGAAAGCACTAC355510.44112992095744963No Hit
CACCCGGCTGTGTGCACATGTGC352560.4374694521469394No Hit
TTAATGCTAATCGTGATAGGGGTT322890.4006538217714014No Hit
TTCAAGTAATTCAGGATAGGTT321080.3984079070096985No Hit
TCGTACGACTCTTAGCGG319360.3962736675676383No Hit
CAACGGAATCCCAAAAGCAGCT302490.37534074931905975No Hit
CCCCCACTGCTAAATTTGACTGGCTT300170.3724620077460483No Hit
TTCTCACTACTGCACTTGACTAG266800.3310552808963111No Hit
ACCCCCCACTGCTAAATTTGACTG240990.2990292808965592No Hit
AACATTCAACGCTGTCGGTGAGTT232260.2881967748912189No Hit
CTTCTCACTACTGCACTTGACTAG230890.2864968283588803No Hit
CATTGCACTTGTCTCGGTCTGA200200.24841554436072516No Hit
CAAAGAATTCTCCTTTTGGGCT197110.24458135838632636No Hit
CCCCCCACTGCTAAATTTGACTGG196990.24443245796013613No Hit
CCCCCCACTGCTAAATTTGACTGGCT192370.2386997915518117No Hit
CCCATAAAGTAGAAAGCACT186790.23177592173396533No Hit
TGTAAACATCCTACACTCTCAGCT185030.22959204881650838No Hit
TGTCAGTTTGTCAAATACCCCAA179730.22301561332643927No Hit
CACTAGATTGTGAGCTCCTGGAA178370.2213280751629498No Hit
GACCCGCGGGCGCTCTCCAGTCCT172270.2137589701649457No Hit
TGAGGTAGTAGTTTGTACAGTT172170.21363488647645384No Hit
AACGGAATCCCAAAAGCAGCTG171330.21259258349312213No Hit
TATTGCACTTGTCCCGGCCTGTAG169250.21001164277249118No Hit
CTCCCACTGCTTCACTTGACTAG165030.20477531111813427No Hit
CACTAGATTGTGAGCTCCTGGA162690.20187175280742448No Hit
TAGCTTATCAGACTGATGTTGACA155010.1923421255312488No Hit
TCGTTTCCCGGCCAATGCACCA154920.19223045021160612No Hit
TAGCAGCACGTAAATATTGGC144090.17879218674793654No Hit
CAAAGAATTCTCCTTTTGGGCTT142070.17628569624040075No Hit
ACTGGACTTGGAGTCAGAAGGCT135160.16771151336561246No Hit
ACTGGACTTGGAGTCAGAAGGCA134980.16748816272632708No Hit
GCCTGTCTGAGCGTCGCT133590.1657633994562901No Hit
CTCCCACTGCTTCACTTGACTAGCCT131990.16377806044042015No Hit
TGAGAACTGAATTCCATGGGTT131220.16282261603903275No Hit
TATTGCACTTGTCCCGGCCTGTAAT123980.15383895699222133No Hit
TGAGAACTGAATTCCATAGGCTGT120520.1495456613704026No Hit
TGAGGTAGTAGTTTGTGCTGTT119950.14883838434599894No Hit
TATTGCACTTGTCCCGGCCTGTT119810.14866466718211033No Hit
TGTCAGTTTGTCAAATACCCCA115670.14352760247854687No Hit
TGTAAACATCCCCGACTGGAAGC113730.14112037892180457No Hit
TTCAAGTAATCCAGGATAGGCTT108720.13490378612836185No Hit
TATTGCACTTGTCCCGGCCTGTAAA108650.13481692754641753No Hit
CCCCCCACTGCTAAATTTGACTGT107330.13317902285832484No Hit
CAACACCAGTCGATGGGCTGT106530.1321863533503899No Hit
ATGCACCTGGGCAAGGATTCTGA104330.1294565122035687No Hit
CCCCCCACTGCTAAATTTGACTGTA97580.12108086323036746No Hit
TATTGCACTTGTCCCGGCCTGTTT96650.11992688492739306No Hit
TCGTTTCCCGGGCGGCGCACCA92410.11466573653533772No Hit
CGCCTGTCTGAGCGTCGCT92290.1145168361091475No Hit
CCCCCCACTGCTAAATTTGACTGA90960.11286652305220561No Hit
AGCAGCATTGTACAGGGCTATGT90810.1126803975194678No Hit
CCCCCCACTGCTAAATTTGAC90520.11232055482284138No Hit
AGCTCGGTCTGAGGCCCCTCAGT86820.10772945834864216No Hit
CAACGGAATCCCAAAAGCAGCTGA86690.10756814955360273No Hit
GGAATACCGGGTGCTGTAGGCTTT86440.10725794033237304No Hit
TTCAAGTAATCCAGGATAGGC85210.10573171096392303No Hit
ATCACATTGCCAGGGATTTCC83660.10380841379229903No Hit
TGAGGTAGTAGGTTGTGTGGTT81050.10056982952266122No Hit
ACTGGACTTGGAGTCAGAAGG80750.1001975784571856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)