FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16250236
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG199409112.271151015899093No Hit
CAACGGAATCCCAAAAGCAGCTG164503110.123120673447449No Hit
CCCCCACTGCTAAATTTGACTG7568804.657655433434936No Hit
AACATTCAACGCTGTCGGTGAGT5963033.6695036305934265No Hit
AAGCTGCCAGTTGAAGAACTGT5952613.6630914160262043No Hit
TAGCAGCACGTAAATATTGGCG5360553.298752091969618No Hit
TTCAAGTAATCCAGGATAGGCT4986033.0682815929565574No Hit
AACATTCAACGCTGTCGGTGAG4100722.523483351257176No Hit
TATTGCACTTGTCCCGGCCTGTAA2219331.3657217039801761No Hit
TGAGGTAGTAGGTTGTATAGTT2026421.2470095818916107No Hit
TATTGCACTTGTCCCGGCCTGTAT2010861.2374343363382538No Hit
TATTGCACTTGTCCCGGCCTGT1697211.0444217548594372No Hit
TAGCTTATCAGACTGATGTTGAC1662311.0229451436889903No Hit
TCGTACGACTCTTAGCGG1646181.0130191339990384No Hit
TAGCTTATCAGACTGATGTTGACT1564980.9630506289262507No Hit
CATAAAGTAGAAAGCACTACT1450680.8927131889038412No Hit
TGTAAACATCCTTGACTGGAAGCT1301080.80065298743969No Hit
TAGCTTATCAGACTGATGTTGA1296170.7976314928595499No Hit
TATTGCACTTGTCCCGGCCTGTA1072120.659756572150706No Hit
TGAGGTAGTAGATTGTATAGTT1056810.6503351705169083No Hit
AGCAGCATTGTACAGGGCTATGA1006450.6193448513609279No Hit
CAACGGAATCCCAAAAGCAGCTGT960280.5909329562967578No Hit
TTCAAGTAATTCAGGATAGGTT905760.5573826743193144No Hit
ACTGGACTTGGAGTCAGAAGGC855010.526152358648822No Hit
TGTAAACATCCCCGACTGGAAGCT807490.496909706419033No Hit
CCCATAAAGTAGAAAGCACTAC766550.4717162261520387No Hit
CAACGGAATCCCAAAAGCAGCT707520.43539059986574963No Hit
CACCCGGCTGTGTGCACATGTGC690280.42478152317295576No Hit
CCCATAAAGTAGAAAGCACT670540.4126340072845711No Hit
TTAATGCTAATCGTGATAGGGGTT668030.411089414332198No Hit
CATTGCACTTGTCTCGGTCTGA664550.4089479069719357No Hit
TTCTCACTACTGCACTTGACTAG536280.33001366872456495No Hit
TGAGGTAGTAGTTTGTACAGTT476590.29328189449064No Hit
TGTCAGTTTGTCAAATACCCCAA454000.2793805579192819No Hit
CGCCTGTCTGAGCGTCGCT453830.27927594405398176No Hit
AACGGAATCCCAAAAGCAGCTG440420.2710237562088329No Hit
CAACACCAGTCGATGGGCTGT422280.2598608414056264No Hit
AACATTCAACGCTGTCGGTGAGTT397660.24471029220744853No Hit
CTTCTCACTACTGCACTTGACTAG385840.23743655169069544No Hit
TGTAAACATCCTACACTCTCAGCT381480.23475351373358513No Hit
TCGTTTCCCGGCCAATGCACCA373640.22992896841621252No Hit
GCCTGTCTGAGCGTCGCT370360.22791053619159746No Hit
ATCCCGGACGAGCCCCCA361740.22260599784520055No Hit
TAGCAGCACGTAAATATTGGC345920.2128707546155022No Hit
TGAGAACTGAATTCCATGGGTT335830.20666161402209787No Hit
TACGCCTGTCTGAGCGTCGCT335120.20622469729055012No Hit
TGAGGTAGTAGTTTGTGCTGTT316810.1949571686220434No Hit
CAAAGAATTCTCCTTTTGGGCT300580.1849696213642682No Hit
CACTAGATTGTGAGCTCCTGGA299460.1842804006046435No Hit
TATTGCACTTGTCCCGGCCTGTT292790.18017584483080737No Hit
TGTCAGTTTGTCAAATACCCCA291250.1792281662863235No Hit
ACCCCCCACTGCTAAATTTGACTG283210.17428054583330357No Hit
CTCCCACTGCTTCACTTGACTAG283010.17415747069765633No Hit
CCCCCCACTGCTAAATTTGACTGG265210.16320378362505011No Hit
TATTGCACTTGTCCCGGCCTGTAG263850.16236687270264874No Hit
CTACGCCTGTCTGAGCGTCGCT263400.16208995364744241No Hit
CACTAGATTGTGAGCTCCTGGAA243450.14981320886662813No Hit
TTCAAGTAATCCAGGATAGGCTT239320.14727170731551223No Hit
TGTAAACATCCCCGACTGGAAGC238720.14690248190857044No Hit
GACCCGCGGGCGCTCTCCAGTCCT232190.14288407872968736No Hit
TTCAAGTAATCCAGGATAGGC230200.14165948112999713No Hit
AGCAGCATTGTACAGGGCTATGT229280.1410933355060197No Hit
ACTGGACTTGGAGTCAGAAGGCA228640.14069949507194848No Hit
TGAGAACTGAATTCCATAGGCTGT228630.14069334131516614No Hit
ACTGGACTTGGAGTCAGAAGGCT227660.14009642690727692No Hit
ACCACAGGGTAGAACCACGGAC225870.13899490444323392No Hit
AGCTCGGTCTGAGGCCCCTCAGT222890.13716108492208975No Hit
CAAAGAATTCTCCTTTTGGGCTT221800.13649032543281217No Hit
CCCCCACTGCTAAATTTGACTGGCTT214590.13205346679272842No Hit
CGTTCGCGCTTTCCCCTG212870.13099502062616197No Hit
CCCATAAAGTAGAAAGCACTA212770.13093348305833835No Hit
TAGCTTATCAGACTGATGTTGACA212260.13061964146243785No Hit
TGATGTAGTAGGTTGTATAGTT208640.12839198150722242No Hit
TGAGGTAGTAGGTTGTGTGGTT208460.12828121388513988No Hit
TTCACAGTGGCTAAGTTCCGC204240.1256843285229827No Hit
TTATAAAGCAATGAGACTGATT200820.12357974370341453No Hit
TTCACAGTGGCTAAGTTCTGC194240.11953057174061965No Hit
ACTGGACTTGGAGTCAGAAGG192470.1184413567901414No Hit
AACATTCAACGCTGTCGGTGA190430.11718599040653932No Hit
TCGTTTCCCGGGCGGCGCACCA185830.11435526228665233No Hit
TTCACAGTGGCTAAGTTCCG180210.1108968509749643No Hit
AGCAGCATTGTACAGGGCTATG176250.10845996328914854No Hit
CAAAGTGCTGTTCGTGCAGGT173090.10651537614592183No Hit
TGAGGTAGTAGGTTGTATAGT172860.10637383973992748No Hit
TAGCAGCACGTAAATATTGGCGT169620.10438002254244184No Hit
AATGACACGATCACTCCCGTTG168560.10372772432351135No Hit
CAAAGTGCTGTTCGTGCAGGTAG166220.10228774523643842No Hit
TATTGCACTTGTCCCGGCCTGTTT165840.10205390247870862No Hit
CAACGGAATCCCAAAAGCAGCTGA165460.10182005972097882No Hit
CCCCCCACTGCTAAATTTGACTGGCT162820.10019546793043498No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)