FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8467306
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG169116019.972822524661325No Hit
CAACGGAATCCCAAAAGCAGCTG86414310.20564273926087No Hit
CCCCCACTGCTAAATTTGACTG5822216.876106756977958No Hit
AACATTCAACGCTGTCGGTGAGT3184523.760960097580033No Hit
AACATTCAACGCTGTCGGTGAG2362392.7900137304592514No Hit
TTCAAGTAATCCAGGATAGGCT2228972.6324429517487613No Hit
AAGCTGCCAGTTGAAGAACTGT2216332.6175149451313087No Hit
TAGCAGCACGTAAATATTGGCG2178952.5733686723971No Hit
TGAGGTAGTAGGTTGTATAGTT923991.0912443698149092No Hit
TAGCTTATCAGACTGATGTTGAC867881.024977720186326No Hit
TATTGCACTTGTCCCGGCCTGTAA838140.989854388160768No Hit
TAGCTTATCAGACTGATGTTGACT787180.9296699564182516No Hit
TATTGCACTTGTCCCGGCCTGTAT658310.7774727876847725No Hit
TATTGCACTTGTCCCGGCCTGT640210.7560964490949069No Hit
TGTAAACATCCTTGACTGGAAGCT565760.6681700177128357No Hit
TAGCTTATCAGACTGATGTTGA564590.6667882322901759No Hit
AGCAGCATTGTACAGGGCTATGA476000.5621622745180108No Hit
TATTGCACTTGTCCCGGCCTGTA455370.5377979725782911No Hit
CATAAAGTAGAAAGCACTACT451910.5337116669693996No Hit
ACTGGACTTGGAGTCAGAAGGC435600.5144493419748856No Hit
CAACGGAATCCCAAAAGCAGCTGT404550.47777888268122115No Hit
CGCCTGTCTGAGCGTCGCT399780.4721454498042234No Hit
TGTAAACATCCCCGACTGGAAGCT385120.4548317965596141No Hit
TTAATGCTAATCGTGATAGGGGTT333240.393560832689878No Hit
TGAGGTAGTAGATTGTATAGTT330250.390029603276414No Hit
CAACGGAATCCCAAAAGCAGCT327380.3866400954447613No Hit
CACCCGGCTGTGTGCACATGTGC275440.3252982707841195No Hit
CATTGCACTTGTCTCGGTCTGA274490.32417630826144705No Hit
TTCAAGTAATTCAGGATAGGTT269030.31772797628903454No Hit
CCCATAAAGTAGAAAGCACT254590.30067414594441255No Hit
AACATTCAACGCTGTCGGTGAGTT241310.28499029089063277No Hit
CAACACCAGTCGATGGGCTGT233450.275707527281995No Hit
TCGTACGACTCTTAGCGG227500.26868049885051987No Hit
CCCATAAAGTAGAAAGCACTAC220860.26083857132363No Hit
AACGGAATCCCAAAAGCAGCTG209090.2469380461743086No Hit
TGTCAGTTTGTCAAATACCCCAA192150.22693168287528523No Hit
CCCCCCACTGCTAAATTTGACTGG165270.19518604854956229No Hit
TTCTCACTACTGCACTTGACTAG162840.1923161865178842No Hit
ACCCCCCACTGCTAAATTTGACTG155570.18373022068648517No Hit
TGAGGTAGTAGTTTGTACAGTT154830.18285627093198237No Hit
TGTAAACATCCTACACTCTCAGCT148880.17582924250050724No Hit
TAGCAGCACGTAAATATTGGC148850.17579381210505443No Hit
CAAAGAATTCTCCTTTTGGGCT139820.16512926307375686No Hit
CACTAGATTGTGAGCTCCTGGA139090.1642671234510717No Hit
CTACGCCTGTCTGAGCGTCGCT136900.16168070458301614No Hit
ACTGGACTTGGAGTCAGAAGGCA135620.16016900771036266No Hit
TGAGGTAGTAGTTTGTGCTGTT132810.15685036066961558No Hit
CTTCTCACTACTGCACTTGACTAG131490.1552914232696917No Hit
CCCCCACTGCTAAATTTGACTGGCTT127710.1508271934426369No Hit
ACTGGACTTGGAGTCAGAAGGCT126780.1497288511835996No Hit
TACGCCTGTCTGAGCGTCGCT126510.14940997762452427No Hit
GACCCGCGGGCGCTCTCCAGTCCT125380.14807543272913487No Hit
CACTAGATTGTGAGCTCCTGGAA124340.1468471790201039No Hit
TCGTTTCCCGGCCAATGCACCA123970.14641020414285252No Hit
ACCACAGGGTAGAACCACGGAC119840.14153261970218156No Hit
TAGCTTATCAGACTGATGTTGACA117510.1387808589886795No Hit
TGAGGTAGTAGGTTGTGTGGTT115530.13644245288879367No Hit
TATTGCACTTGTCCCGGCCTGTT114470.1351905789161275No Hit
ACTGGACTTGGAGTCAGAAGG109050.12878948747098545No Hit
TGTCAGTTTGTCAAATACCCCA106430.1256952329347729No Hit
TTCACAGTGGCTAAGTTCCG106080.1252818783211567No Hit
TATTGCACTTGTCCCGGCCTGTAG104190.12304976340762928No Hit
GGAATACCGGGTGCTGTAGGCTTT98360.11616445655796542No Hit
TTCACAGTGGCTAAGTTCTG98170.11594006405343094No Hit
CTCCCACTGCTTCACTTGACTAG98070.11582196273525486No Hit
TGATGTAGTAGGTTGTATAGTT98000.11573929181253163No Hit
TTCAAGTAATCCAGGATAGGCTT94780.11193642936726272No Hit
TGTAAACATCCCCGACTGGAAGC93840.11082627697640784No Hit
AGCAGCATTGTACAGGGCTATG92460.10919647878557831No Hit
CAAAGAATTCTCCTTTTGGGCTT92170.10885398496286777No Hit
ACGCCTGTCTGAGCGTCGCT91010.10748400967202557No Hit
TTCAAGTAATCCAGGATAGGC90930.10738952861748471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)