FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6862125
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG81167211.828289341858389No Hit
CAACGGAATCCCAAAAGCAGCTG5332537.770960161757473No Hit
CCCCCACTGCTAAATTTGACTG3022524.404641419385395No Hit
TTCAAGTAATCCAGGATAGGCT2533213.691582417982768No Hit
AACATTCAACGCTGTCGGTGAGT2423023.531005337267975No Hit
AAGCTGCCAGTTGAAGAACTGT2378403.465981747636483No Hit
TAGCAGCACGTAAATATTGGCG2096593.055307211687342No Hit
AACATTCAACGCTGTCGGTGAG1516032.2092719092117967No Hit
TATTGCACTTGTCCCGGCCTGTAA833891.2152066597446127No Hit
TGAGGTAGTAGGTTGTATAGTT781301.1385685920906425No Hit
TAGCTTATCAGACTGATGTTGAC754501.0995136346248429No Hit
TATTGCACTTGTCCCGGCCTGTAT704551.0267227717361604No Hit
TATTGCACTTGTCCCGGCCTGT642030.9356139679763922No Hit
CATAAAGTAGAAAGCACTACT623680.9088729803085779No Hit
TAGCTTATCAGACTGATGTTGACT601040.8758802848971711No Hit
TGTAAACATCCTTGACTGGAAGCT520100.7579284842523271No Hit
TAGCTTATCAGACTGATGTTGA504170.7347141009526932No Hit
TATTGCACTTGTCCCGGCCTGTA451630.6581488970253384No Hit
TGAGGTAGTAGATTGTATAGTT404650.5896861394976046No Hit
TGTAAACATCCCCGACTGGAAGCT361110.5262364063610033No Hit
AGCAGCATTGTACAGGGCTATGA353900.5157294569830775No Hit
CAACGGAATCCCAAAAGCAGCTGT344960.5027014226642622No Hit
CAACGGAATCCCAAAAGCAGCT323500.4714283112009764No Hit
TCGTACGACTCTTAGCGG307270.4477767455416507No Hit
ACTGGACTTGGAGTCAGAAGGC302730.44116071916498173No Hit
CCCATAAAGTAGAAAGCACTAC296230.43168843470499296No Hit
CCCATAAAGTAGAAAGCACT273150.3980545384993715No Hit
CGCCTGTCTGAGCGTCGCT268660.39151137584931783No Hit
TTCAAGTAATTCAGGATAGGTT250740.3653970162303951No Hit
TTCTCACTACTGCACTTGACTAG238280.3472393755578629No Hit
CACCCGGCTGTGTGCACATGTGC236140.34412080805872813No Hit
CATTGCACTTGTCTCGGTCTGA197150.287301674044119No Hit
TGTAAACATCCTACACTCTCAGCT193800.28241980436089403No Hit
CTCCCACTGCTTCACTTGACTAG191430.2789660637193289No Hit
CTTCTCACTACTGCACTTGACTAG184700.26915860611690984No Hit
TGAGGTAGTAGTTTGTACAGTT176610.2573692551505547No Hit
CAACACCAGTCGATGGGCTGT167280.24377288376414014No Hit
TCGTTTCCCGGCCAATGCACCA165830.2416598356922965No Hit
AACATTCAACGCTGTCGGTGAGTT161060.23470863617319707No Hit
CCCCCACTGCTAAATTTGACTGGCTT157460.22946244785689565No Hit
TGATGTAGTAGGTTGTATAGTT148180.21593894019709636No Hit
CAAAGAATTCTCCTTTTGGGCT146460.21343242800153012No Hit
CCCCCCACTGCTAAATTTGACTGG140490.2047324990436636No Hit
TGAGGTAGTAGTTTGTGCTGTT134130.1954642330181977No Hit
ACCCCCCACTGCTAAATTTGACTG132200.1926516931708472No Hit
AACGGAATCCCAAAAGCAGCTG126210.18392261872233456No Hit
CCCCCCACTGCTAAATTTGACTGGCT123540.18003169572107766No Hit
TTCAAGTAATCCAGGATAGGC118310.1724101499171175No Hit
TTCACAGTGGCTAAGTTCTGC117150.1707197114596426No Hit
CAACGGAATCCCAAAAGCAGCTGA115740.1686649543690912No Hit
CTACGCCTGTCTGAGCGTCGCT114980.1675574257245387No Hit
TTCACAGTGGCTAAGTTCCG113610.16556095961527953No Hit
TAGCAGCACGTAAATATTGGC109080.15895950598393355No Hit
AGCTCGGTCTGAGGCCCCTCAGT108720.15843488715230342No Hit
TGTAAACATCCCCGACTGGAAGC107450.15658414849627483No Hit
GGAATACCGGGTGCTGTAGGCTTT107380.15648213927901342No Hit
TATTGCACTTGTCCCGGCCTGTT104940.15292638942018688No Hit
TATTGCACTTGTCCCGGCCTGTAG103460.15076962311237407No Hit
GACCCGCGGGCGCTCTCCAGTCCT101400.1477676375758238No Hit
TAGCTTATCAGACTGATGTTGACA101110.1473450279614551No Hit
ATCCCGGACGAGCCCCCA99940.14564001675865712No Hit
AATGCACCCGGGCAAGGATTCT98730.1438767145745669No Hit
TGAGGTAGTAGGTTGTGTGGTT95940.13981091862943332No Hit
CAAAGAATTCTCCTTTTGGGCTT95510.13918429058054174No Hit
TTCAAGTAATCCAGGATAGGCTT95300.1388782629287575No Hit
CACTAGATTGTGAGCTCCTGGA93710.13656119642239103No Hit
GCCTGTCTGAGCGTCGCT93390.1360948685720531No Hit
TGTAAACATCCTACACTCAGCT92110.13422955717070148No Hit
ACCACAGGGTAGAACCACGGAC91810.1337923748110097No Hit
AACATTCAACGCTGTCGGTGA84850.12364974406616028No Hit
GGAATACCGGGTGCTGTAGGCTT84430.12303768876259177No Hit
GTCAGGATGGCCGAGCGGTCTAAGG83420.12156584148496274No Hit
TACGCCTGTCTGAGCGTCGCT83260.1213326775597938No Hit
TGATGTAGTAGATTGTATAGTT80070.1166839718017378No Hit
TTCACAGTGGCTAAGTTCCGC79930.11647995336721496No Hit
CACTAGATTGTGAGCTCCTGGAA79660.11608648924349237No Hit
ACTGGACTTGGAGTCAGAAGGCT79090.11525584276007796No Hit
TTCACAGTGGCTAAGTTCTG74430.10846494343953221No Hit
TAGCAGCACATCATGGTTTACA73280.10678907772738036No Hit
TGAGGTAGTAGGTTGTATAGT72640.10585642202670456No Hit
ACTGGACTTGGAGTCAGAAGG72340.10541923966701278No Hit
CAAAGTGCTGTTCGTGCAGGT71010.10348106453904585No Hit
ACGCCTGTCTGAGCGTCGCT70830.10321875512323078No Hit
TTATAAAGCAATGAGACTGATT69350.10106198881541797No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)