FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5543960
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG70857112.780954408040463No Hit
CAACGGAATCCCAAAAGCAGCTG61698111.128886211300227No Hit
CCCCCACTGCTAAATTTGACTG1791483.231408596021616No Hit
AACATTCAACGCTGTCGGTGAGT1665773.00465732075989No Hit
TAGCAGCACGTAAATATTGGCG1293962.333999523806088No Hit
AACATTCAACGCTGTCGGTGAG1268592.288238010375255No Hit
TTCAAGTAATCCAGGATAGGCT1146152.0673850460681535No Hit
AAGCTGCCAGTTGAAGAACTGT949901.7133962005497876No Hit
TATTGCACTTGTCCCGGCCTGTAA768921.386950843801182No Hit
TATTGCACTTGTCCCGGCCTGTAT715941.2913873837473575No Hit
TAGCTTATCAGACTGATGTTGAC648801.170282613871673No Hit
TAGCTTATCAGACTGATGTTGACT609521.099430731823462No Hit
TAGCTTATCAGACTGATGTTGA458310.8266834537045721No Hit
TATTGCACTTGTCCCGGCCTGT423700.7642551533560848No Hit
GCCTGTCTGAGCGTCGCT404110.7289194005728757No Hit
CAACGGAATCCCAAAAGCAGCTGT319760.5767718381806506No Hit
CCCCCACTGCTAAATTTGACTGGCTT311150.5612414230982907No Hit
TATTGCACTTGTCCCGGCCTGTA300450.5419411395464614No Hit
TGAGGTAGTAGGTTGTATAGTT286690.5171213356517724No Hit
TGTAAACATCCTTGACTGGAAGCT267780.4830121429447543No Hit
AGCAGCATTGTACAGGGCTATGA225120.4060635358119467No Hit
CAACGGAATCCCAAAAGCAGCT223740.40357434036320605No Hit
CACCCGGCTGTGTGCACATGTGC221480.3994978318746889No Hit
TGTAAACATCCCCGACTGGAAGCT213300.384743035664038No Hit
ACTGGACTTGGAGTCAGAAGGC195630.3528705113312506No Hit
CCCCCCACTGCTAAATTTGACTGGCT181790.3279064062511273No Hit
CTCCCACTGCTTCACTTGACTAG179680.32410046248529933No Hit
CTCCCACTGCTTCACTTGACTAGCCT178840.3225853000382398No Hit
ACCCCCCACTGCTAAATTTGACTG175600.31674110202815314No Hit
CATAAAGTAGAAAGCACTACT173300.3125924429469188No Hit
CCCATAAAGTAGAAAGCACTAC155570.28061169272505576No Hit
ACCGGGTGCTGTAGGCTT153980.2777437066645503No Hit
TTCTCACTACTGCACTTGACTAG148870.26852646844493827No Hit
CATTGCACTTGTCTCGGTCTGA145550.26253796924941736No Hit
TTCAAGTAATTCAGGATAGGTT145180.261870576266784No Hit
CTTCTCACTACTGCACTTGACTAG142700.2573972395183226No Hit
TGAGGTAGTAGATTGTATAGTT142060.2562428300348487No Hit
CCCCCCACTGCTAAATTTGACTGG129140.23293818858721926No Hit
TCCTGTCTGAGCGTCGCT124160.2239554397939379No Hit
ACCGGGTGCTGTAGGCTTA122960.2217909220124243No Hit
CTACGCCTGTCTGAGCGTCGCT122580.2211054913816117No Hit
AACGGAATCCCAAAAGCAGCTG122320.22063651252895042No Hit
TACGCCTGTCTGAGCGTCGCT113030.2038795373703995No Hit
TCGTACGACTCTTAGCGG110150.1986846946947669No Hit
AACATTCAACGCTGTCGGTGAGTT106880.19278638374014243No Hit
ACCGGGTGCTGTAGGCTTT105690.19063990360680813No Hit
TAGCTTATCAGACTGATGTTGACA105150.18966587060512702No Hit
CAACACCAGTCGATGGGCTGT96700.17442405789363558No Hit
TATTGCACTTGTCCCGGCCTGTAG94980.17132158240679948No Hit
CAAAGAATTCTCCTTTTGGGCT91710.16542327145217497No Hit
TGTAAACATCCTACACTCTCAGCT90770.16372773252332268No Hit
GGAATACCGGGTGCTGTAGGCTTT88430.1595069228493712No Hit
TATTGCACTTGTCCCGGCCTGTT87170.15723417917878194No Hit
CCCATAAAGTAGAAAGCACT86840.1566389367888657No Hit
TAGCAGCACGTAAATATTGGC84520.1524542024112728No Hit
GGAATACCGGGTGCTGTAGGCTT84250.15196718591043226No Hit
CACTAGATTGTGAGCTCCTGGA82460.14873844688634116No Hit
CACTAGATTGTGAGCTCCTGGAA76980.13885381568409585No Hit
CGCCTGTCTGAGCGTCGCT74710.13475926954739934No Hit
TCGTTTCCCGGCCAATGCACCA73330.1322700740986587No Hit
TTCACAGTGGCTAAGTTCCGC72350.1305023845770893No Hit
CGCGACCTCAGATCAGACGTGGCG69000.12445977243703057No Hit
AGCTCGGTCTGAGGCCCCTCAGT68340.1232692876571981No Hit
TATTGCACTTGTCCCGGCCTGTAAT65790.11866968737148176No Hit
TATTGCACTTGTCCCGGCCTGTTT64340.11605422838548618No Hit
TGAGGTAGTAGTTTGTACAGTT63940.11533272245831501No Hit
CAACGGAATCCCAAAAGCAGCTGA62840.11334858115859421No Hit
CGCCCCACTGCTAAATTTGACTG62770.11322231762133925No Hit
CCTTGGCTTGGCTCTTGACGTGGACA60300.10876701852105715No Hit
ACTGGACTTGGAGTCAGAAGGCT59800.10786513611209317No Hit
AACATTCAACGCTGTCGGTGAGA59630.1075584960930454No Hit
ATGCACCTGGGCAAGGATTCTGA59000.10642212425775077No Hit
TGTAAACATCCCCGACTGGAAGC56970.10276048167735699No Hit
TAGCAGCACATCATGGTTTACA56960.1027424440291777No Hit
TGAGGTAGTAGTTTGTGCTGTT56410.1017503733793173No Hit
ACTGGACTTGGAGTCAGAAGGCA55500.10010894739500284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)