FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6193700
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5933519.579911845907938No Hit
CAACGGAATCCCAAAAGCAGCTG4988208.053667436265883No Hit
AACATTCAACGCTGTCGGTGAGT2519474.067794694609038No Hit
CCCCCACTGCTAAATTTGACTG2016733.2560989392447164No Hit
AACATTCAACGCTGTCGGTGAG1658342.6774625829471885No Hit
TAGCAGCACGTAAATATTGGCG1334882.1552222419555354No Hit
AAGCTGCCAGTTGAAGAACTGT1220841.9710996657894313No Hit
TTCAAGTAATCCAGGATAGGCT1131451.8267755945557584No Hit
TAGCTTATCAGACTGATGTTGAC685321.1064791643121237No Hit
CGCCTGTCTGAGCGTCGCT672341.0855223856499345No Hit
TAGCTTATCAGACTGATGTTGA631131.0189870352132004No Hit
TATTGCACTTGTCCCGGCCTGTAA621801.0039233414598705No Hit
TATTGCACTTGTCCCGGCCTGT605650.9778484589179327No Hit
TAGCTTATCAGACTGATGTTGACT580200.9367583189369844No Hit
TATTGCACTTGTCCCGGCCTGTAT552570.8921484734488271No Hit
ACCGGGTGCTGTAGGCTT506010.8169753136251352No Hit
TGAGGTAGTAGGTTGTATAGTT415020.6700679722944282No Hit
TATTGCACTTGTCCCGGCCTGTA351670.567786621889985No Hit
ACCGGGTGCTGTAGGCTTT325080.5248559019648998No Hit
ACCGGGTGCTGTAGGCTTA302050.4876729580057155No Hit
AGCAGCATTGTACAGGGCTATGA279360.4510389589421509No Hit
ACTGGACTTGGAGTCAGAAGGC271580.43847780809532266No Hit
CATAAAGTAGAAAGCACTACT269050.43439301225438753No Hit
TCGTACGACTCTTAGCGG264800.4275312010591408No Hit
TGTAAACATCCTTGACTGGAAGCT249570.4029416988229975No Hit
TGTAAACATCCCCGACTGGAAGCT231000.37295962025929574No Hit
CACCCGGCTGTGTGCACATGTGC228110.36829358864652795No Hit
CAACGGAATCCCAAAAGCAGCTGT217170.35063047935805736No Hit
TGAGGTAGTAGATTGTATAGTT209420.3381177648255485No Hit
CATTGCACTTGTCTCGGTCTGA207260.3346303501945525No Hit
CAACACCAGTCGATGGGCTGT199940.3228118895006216No Hit
CTACGCCTGTCTGAGCGTCGCT184760.29830311445501073No Hit
CCCATAAAGTAGAAAGCACT182250.29425060949028853No Hit
TTCAAGTAATTCAGGATAGGTT168440.27195375946526307No Hit
AACATTCAACGCTGTCGGTGAGTT162650.262605550801621No Hit
CAACGGAATCCCAAAAGCAGCT161580.2608779889242295No Hit
TACGCCTGTCTGAGCGTCGCT158750.2563088299401004No Hit
CCCATAAAGTAGAAAGCACTAC146120.23591714161163763No Hit
CTCCCACTGCTTCACTTGACTAG142530.2301209293314174No Hit
ACGCCTGTCTGAGCGTCGCT142120.22945896636905241No Hit
TTCTCACTACTGCACTTGACTAG135840.21931963123819365No Hit
AACGGAATCCCAAAAGCAGCTG126710.20457884624699293No Hit
GCGTTCGCGCTTTCCCCTG122200.19729725366097808No Hit
CTTCTCACTACTGCACTTGACTAG117070.18901464391236256No Hit
CCCCCCACTGCTAAATTTGACTGG116160.18754540904467443No Hit
CGCGACCTCAGATCAGACGTGG115220.1860277378626669No Hit
CCCCCACTGCTAAATTTGACTGGCTT109120.17617902061772445No Hit
GCCTGTCTGAGCGTCGCT105690.17064113534720765No Hit
CTCCCACTGCTTCACTTGACTAGCCT97570.15753103960475967No Hit
TGTCAGTTTGTCAAATACCCCAA96520.15583576860358106No Hit
ACCCCCCACTGCTAAATTTGACTG94910.15323635306844052No Hit
TGAGGTAGTAGTTTGTACAGTT92570.14945832055152816No Hit
TATTGCACTTGTCCCGGCCTGTT92570.14945832055152816No Hit
CCCCCCACTGCTAAATTTGACTGGCT92300.14902239372265366No Hit
CACTAGATTGTGAGCTCCTGGA89680.14479228893876037No Hit
GGAATACCGGGTGCTGTAGGCTT88300.14256421848006845No Hit
GGAATACCGGGTGCTGTAGGCTTT83970.13557324377996996No Hit
ACCACAGGGTAGAACCACGGAC82670.13347433682612977No Hit
TATTGCACTTGTCCCGGCCTGTAG80870.13056815796696644No Hit
TGAGGTAGTAGTTTGTGCTGTT79070.12766197910780308No Hit
CGCGACCTCAGATCAGACGTGGCG77450.12504641813455608No Hit
TGAGGTAGTAGGTTGTGTGGTT75540.12196263945622164No Hit
TCGTTTCCCGGCCAATGCACCA75100.12125224017953727No Hit
TAGCTTATCAGACTGATGTTGACA73690.118975733406526No Hit
TGTAAACATCCTACACTCTCAGCT72940.11776482554854127No Hit
TAGCAGCACGTAAATATTGGC72550.1171351534623892No Hit
ATCCCGGACGAGCCCCCA71530.11548831877552998No Hit
ATCCCACCGCTGCCACCA69210.11174257713483056No Hit
ACTGGACTTGGAGTCAGAAGGCT68360.11037021489578121No Hit
TTAATGCTAATCGTGATAGGGGTT67660.10924003422832877No Hit
TGTAAACATCCCCGACTGGAAGC67280.1086265075802832No Hit
AACATTCAACGCTGTCGGTGA64540.10420265753911231No Hit
TAGTGGGTTATCAGAACT64420.10400891228183476No Hit
CACTAGATTGTGAGCTCCTGGAA63200.10203916883284629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)