FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14649912
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG12210778.335046654205158No Hit
CAACGGAATCCCAAAAGCAGCTG11540667.877630937305288No Hit
AACATTCAACGCTGTCGGTGAGT4485503.06179313568573No Hit
CCCCCACTGCTAAATTTGACTG3667722.5035781784900824No Hit
AAGCTGCCAGTTGAAGAACTGT2997832.0463126331407318No Hit
AACATTCAACGCTGTCGGTGAG2966782.025117966578912No Hit
TAGCAGCACGTAAATATTGGCG2362461.6126103692636518No Hit
TTCAAGTAATCCAGGATAGGCT2327631.5888354824247406No Hit
TAGCTTATCAGACTGATGTTGAC2202061.5031216569765062No Hit
TATTGCACTTGTCCCGGCCTGTAA1868281.2752841109216217No Hit
TAGCTTATCAGACTGATGTTGACT1647201.1243753546096387No Hit
TATTGCACTTGTCCCGGCCTGT1591371.0862659106757775No Hit
TATTGCACTTGTCCCGGCCTGTAT1578911.0777607401327736No Hit
TAGCTTATCAGACTGATGTTGA1541591.0522861843811757No Hit
TCGTACGACTCTTAGCGG1067020.7283456719740024No Hit
ACCGGGTGCTGTAGGCTT1025330.6998881631507411No Hit
TGAGGTAGTAGGTTGTATAGTT948810.6476557674885692No Hit
TATTGCACTTGTCCCGGCCTGTA922230.6295123137941033No Hit
ACTGGACTTGGAGTCAGAAGGC864040.5897919386819525No Hit
AGCAGCATTGTACAGGGCTATGA862550.5887748677261679No Hit
CGCCTGTCTGAGCGTCGCT784030.5351772761501912No Hit
ACCGGGTGCTGTAGGCTTT672600.4591153858125564No Hit
TGTAAACATCCCCGACTGGAAGCT638180.4356203641359757No Hit
TGTAAACATCCTTGACTGGAAGCT633850.4326647149825883No Hit
GGAATACCGGGTGCTGTAGGCTTT616300.42068512083895115No Hit
CACCCGGCTGTGTGCACATGTGC594490.4057976593989097No Hit
CAACGGAATCCCAAAAGCAGCTGT578010.39454844506915815No Hit
ACCGGGTGCTGTAGGCTTA557960.3808623560332649No Hit
GGAATACCGGGTGCTGTAGGCTT502560.34304642922087175No Hit
CATTGCACTTGTCTCGGTCTGA456690.3117356609377585No Hit
CAACGGAATCCCAAAAGCAGCT442330.30193355427664004No Hit
CCCCCACTGCTAAATTTGACTGGCTT428940.29279356763371683No Hit
TGAGGTAGTAGATTGTATAGTT406570.27752385133781005No Hit
TTCAAGTAATTCAGGATAGGTT404220.27591974613908943No Hit
TTAATGCTAATCGTGATAGGGGTT402160.2745135943478705No Hit
CATAAAGTAGAAAGCACTACT366040.2498581561445557No Hit
CTCCCACTGCTTCACTTGACTAG361940.24705950452125586No Hit
CGCGACCTCAGATCAGACGTGG351210.23973522844369302No Hit
CACTAGATTGTGAGCTCCTGGA326970.2231890539683788No Hit
AACATTCAACGCTGTCGGTGAGTT320990.21910711818610243No Hit
CAACACCAGTCGATGGGCTGT320960.21908664024739535No Hit
CGCGACCTCAGATCAGACGTGGCG316710.21618559893056014No Hit
CCCCCCACTGCTAAATTTGACTGGCT314020.21434941042649266No Hit
AACGGAATCCCAAAAGCAGCTG303290.20702513434892986No Hit
CTACGCCTGTCTGAGCGTCGCT299480.20442443613313172No Hit
TAGCTTATCAGACTGATGTTGACA291260.19881348092739398No Hit
TCGTTTCCCGGCCAATGCACCA286440.19552335877512436No Hit
TTCTCACTACTGCACTTGACTAG282960.19314791788510402No Hit
CCCCCCACTGCTAAATTTGACTGG281620.1922332366228548No Hit
CTCCCACTGCTTCACTTGACTAGCCT279400.19071786915853148No Hit
CCCATAAAGTAGAAAGCACTAC274230.18718883772134604No Hit
TATTGCACTTGTCCCGGCCTGTT269640.18405571309916402No Hit
TACGCCTGTCTGAGCGTCGCT269040.18364615432502257No Hit
ACCCCCCACTGCTAAATTTGACTG268780.18346867885622795No Hit
CTTCTCACTACTGCACTTGACTAG260840.17804885107842286No Hit
CACTAGATTGTGAGCTCCTGGAA253030.1727177610350151No Hit
TATTGCACTTGTCCCGGCCTGTAG247460.1689156904150687No Hit
TGAGGTAGTAGTTTGTGCTGTT247230.16875869288498116No Hit
TGAATACCGGGTGCTGTAGGCTTT237690.16224670837613223No Hit
AGCTCGGTCTGAGGCCCCTCAGT227080.15500434405339772No Hit
CCCATAAAGTAGAAAGCACT224120.1529838541009666No Hit
GCGTTCGCGCTTTCCCCTG205500.14027388014344389No Hit
CGCGACCTCAGATCAGACGTGGC203300.1387721646382586No Hit
TGAGGTAGTAGGTTGTGTGGTT199920.13646498354392844No Hit
TGAATACCGGGTGCTGTAGGCTT195950.13375506965502593No Hit
GGGAATACCGGGTGCTGTAGGCTT190790.1302328641974095No Hit
ACCACAGGGTAGAACCACGGAC178910.12212360046940897No Hit
CAAAGAATTCTCCTTTTGGGCT171230.1168812481603985No Hit
TGTAAACATCCCCGACTGGAAGC170070.11608943453039172No Hit
TGAGGTAGTAGTTTGTACAGTT168930.11531127285952297No Hit
GGGAATACCGGGTGCTGTAGGCTTT163480.11159111399440488No Hit
ACGCCTGTCTGAGCGTCGCT162530.1109426459353476No Hit
TTCAAGTAATCCAGGATAGGCTT154000.1051200853629701No Hit
TGTAAACATCCTACACTCTCAGCT149160.10181631125156247No Hit
ACTGGACTTGGAGTCAGAAGGCT148340.10125658092690251No Hit
TATTGCACTTGTCCCGGCCTGTAAT148220.10117466917207421No Hit
CAAAGTGCTGTTCGTGCAGGTAG147140.10043746337861963No Hit
TAGCAGCACGTAAATATTGGC146830.10022585801197986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)