FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5761757
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG58563210.16412181214862No Hit
CAACGGAATCCCAAAAGCAGCTG3846616.6761059169971935No Hit
AACATTCAACGCTGTCGGTGAGT1602172.7806969297733315No Hit
CCCCCACTGCTAAATTTGACTG1550612.691210337402289No Hit
TTCAAGTAATCCAGGATAGGCT1252002.1729482864341554No Hit
TAGCAGCACGTAAATATTGGCG1023821.7769232544864355No Hit
AACATTCAACGCTGTCGGTGAG962091.6697857962423617No Hit
AAGCTGCCAGTTGAAGAACTGT941241.6335989178300994No Hit
TATTGCACTTGTCCCGGCCTGTAA822311.4271861864358388No Hit
TATTGCACTTGTCCCGGCCTGTAT776571.3478006795496582No Hit
TAGCTTATCAGACTGATGTTGAC534040.92687005022947No Hit
TAGCTTATCAGACTGATGTTGACT504930.8763472669881774No Hit
TATTGCACTTGTCCCGGCCTGT482540.8374875927603334No Hit
TAGCTTATCAGACTGATGTTGA436590.7577376137174824No Hit
TATTGCACTTGTCCCGGCCTGTA380150.6597813826581024No Hit
GGAATACCGGGTGCTGTAGGCTTT349310.6062560430785262No Hit
TGAGGTAGTAGGTTGTATAGTT329480.5718394579986625No Hit
CCCCCACTGCTAAATTTGACTGGCTT297310.5160057947601747No Hit
GGAATACCGGGTGCTGTAGGCTT261880.4545141351848056No Hit
CTCCCACTGCTTCACTTGACTAG255820.4439965100923208No Hit
CAACGGAATCCCAAAAGCAGCTGT246890.42849776552534236No Hit
ACCGGGTGCTGTAGGCTT236050.40968405991436296No Hit
TGTAAACATCCTTGACTGGAAGCT230460.39998215822014016No Hit
AGCAGCATTGTACAGGGCTATGA224670.38993314018623143No Hit
ACCGGGTGCTGTAGGCTTA222240.3857156766590469No Hit
CCCCCCACTGCTAAATTTGACTGGCT218500.3792246011069193No Hit
TTCTCACTACTGCACTTGACTAG218460.3791551778389821No Hit
TGTAAACATCCCCGACTGGAAGCT214020.37144919509795365No Hit
ACTGGACTTGGAGTCAGAAGGC213780.3710326554903305No Hit
CATAAAGTAGAAAGCACTACT207340.3598555093524423No Hit
CCCATAAAGTAGAAAGCACTAC206050.35761660896146785No Hit
CTACGCCTGTCTGAGCGTCGCT203630.3534165012512676No Hit
CTTCTCACTACTGCACTTGACTAG194600.3377441985144462No Hit
CTCCCACTGCTTCACTTGACTAGCCT186390.32349507277033723No Hit
ACCGGGTGCTGTAGGCTTT184330.31991977447157177No Hit
ACCCCCCACTGCTAAATTTGACTG181060.3142444223177062No Hit
CACCCGGCTGTGTGCACATGTGC178590.3099575355225845No Hit
CCCCCCACTGCTAAATTTGACTGG176110.3056532929104785No Hit
TGAGGTAGTAGATTGTATAGTT172390.29919692899231953No Hit
TGAATACCGGGTGCTGTAGGCTTT158360.2748467177633489No Hit
TTAATGCTAATCGTGATAGGGGTT150060.2604413896663813No Hit
TTCAAGTAATTCAGGATAGGTT147160.25540820274093473No Hit
CATTGCACTTGTCTCGGTCTGA124750.2165138168791221No Hit
AACATTCAACGCTGTCGGTGAGTT123240.21389308851449307No Hit
TGAATACCGGGTGCTGTAGGCTT118400.20549287309409264No Hit
CGCCTGTCTGAGCGTCGCT112340.19497524800160784No Hit
TCGTACGACTCTTAGCGG107490.18655767676422313No Hit
TATTGCACTTGTCCCGGCCTGTAG107130.1859328673527884No Hit
AGCTCGGTCTGAGGCCCCTCAGT104690.1816980480086196No Hit
GTCAGGATGGCCGAGCGGTCTAAGG104230.18089968042734186No Hit
TAGCAGCACATCATGGTTTACA98310.17062503677263724No Hit
TATTGCACTTGTCCCGGCCTGTT93730.1626760725938286No Hit
TGTAAACATCCTACACTCTCAGCT90140.15644533429646545No Hit
CCCATAAAGTAGAAAGCACT87080.15113445429927017No Hit
CAAAGAATTCTCCTTTTGGGCT86120.14946829586877752No Hit
CACTAGATTGTGAGCTCCTGGA83430.14479958110000127No Hit
TAGCTTATCAGACTGATGTTGACA75500.13103641823145265No Hit
TGAGGTAGTAGTTTGTACAGTT75130.13039425300303362No Hit
CACTAGATTGTGAGCTCCTGGAA74450.12921405744810133No Hit
AACGGAATCCCAAAAGCAGCTG73360.12732227339681282No Hit
GGGAATACCGGGTGCTGTAGGCTT70430.12223701902041338No Hit
CGCGACCTCAGATCAGACGTGGCG69920.12135187235421419No Hit
CAACACCAGTCGATGGGCTGT69830.12119567000135549No Hit
TATTGCACTTGTCCCGGCCTGTTT68610.1190782603292711No Hit
TACGCCTGTCTGAGCGTCGCT67570.11727325536290405No Hit
CCCCCACTGCTAAATTTGACTGGCT65950.11446161301144771No Hit
TGAGGTAGTAGGTTGTGTGGTT64620.11215328935253605No Hit
GGGCTACGCCTGTCTGAGCGTCGCT64500.11194501954872446No Hit
GGAATACCGGGTGCTGTAGGCTTA64020.11111194033347814No Hit
TGTAAACATCCCCGACTGGAAGC62120.10781433510646143No Hit
TCCCACTGCTTCACTTGACTAG61910.10744986294979118No Hit
TATTGCACTTGTCCCGGCCTGTAAT61620.10694654425724653No Hit
TAGCAGCACGTAAATATTGGC58760.1019827805997372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)