FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9242562
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6435346.962723106428716No Hit
CCCCCCACTGCTAAATTTGACTG6150386.654410324756274No Hit
AACATTCAACGCTGTCGGTGAGT3838564.153134163449486No Hit
TTCAAGTAATCCAGGATAGGCT2530142.737487722560043No Hit
CCCCCACTGCTAAATTTGACTG2169332.3471089509597016No Hit
AACATTCAACGCTGTCGGTGAG2048532.216409259683625No Hit
AAGCTGCCAGTTGAAGAACTGT2022832.1886031167548565No Hit
TAGCAGCACGTAAATATTGGCG1741021.8836984810055912No Hit
TATTGCACTTGTCCCGGCCTGTAA987721.068664727377539No Hit
TAGCTTATCAGACTGATGTTGA893300.9665069057692013No Hit
TAGCTTATCAGACTGATGTTGAC890010.9629472866938842No Hit
TATTGCACTTGTCCCGGCCTGT881040.9532421854459836No Hit
TATTGCACTTGTCCCGGCCTGTAT837050.9056471571410611No Hit
TGAGGTAGTAGGTTGTATAGTT834010.9023580258374247No Hit
CGCCTGTCTGAGCGTCGCT785810.8502079834574007No Hit
TAGCTTATCAGACTGATGTTGACT761190.8235703477022929No Hit
ACCGGGTGCTGTAGGCTT733360.7934596489588059No Hit
CATAAAGTAGAAAGCACTACT645140.6980099240881479No Hit
TATTGCACTTGTCCCGGCCTGTA510630.5524766834131056No Hit
GCCTGTCTGAGCGTCGCT468750.5071645719011676No Hit
CTACGCCTGTCTGAGCGTCGCT448580.485341618481975No Hit
TGAGGTAGTAGATTGTATAGTT428630.4637566943018613No Hit
ACCGGGTGCTGTAGGCTTA426050.4609652604981173No Hit
CCCATAAAGTAGAAAGCACTAC421180.4556961587057788No Hit
ACCGGGTGCTGTAGGCTTT419920.45433290033650836No Hit
ACTGGACTTGGAGTCAGAAGGC407980.4414144043610419No Hit
CAACGGAATCCCAAAAGCAGCTGT400800.4336459955583744No Hit
AGCAGCATTGTACAGGGCTATGA382720.4140843199104317No Hit
TGTAAACATCCTTGACTGGAAGCT360900.390476147198147No Hit
CTCCCACTGCTTCACTTGACTAG351060.3798297485047977No Hit
GTCAGGATGGCCGAGCGGTCTAAGG328620.35555076611874503No Hit
TGTAAACATCCCCGACTGGAAGCT326890.3536789907387151No Hit
CCCCCACTGCTAAATTTGACTGGCTT323090.34956757660916965No Hit
CACCCGGCTGTGTGCACATGTGC321200.3475226890552641No Hit
CCCATAAAGTAGAAAGCACT272670.2950156028166216No Hit
AACATTCAACGCTGTCGGTGAGTT266950.28882684260056896No Hit
CATTGCACTTGTCTCGGTCTGA256470.27748799521171724No Hit
TTCTCACTACTGCACTTGACTAG253040.2737769029842591No Hit
CAACGGAATCCCAAAAGCAGCT240540.26025251440022795No Hit
TACGCCTGTCTGAGCGTCGCT232640.2517051008151203No Hit
CCCCCCACTGCTAAATTTGACTGGCT230750.2496602132612148No Hit
TTCAAGTAATTCAGGATAGGTT230220.24908677918525188No Hit
CAACACCAGTCGATGGGCTGT217970.23583287837290137No Hit
TCCACCACGTTCCCGTGG216910.23468601022097552No Hit
ACGCCTGTCTGAGCGTCGCT216070.23377717130812864No Hit
CTTCTCACTACTGCACTTGACTAG202240.2188137877787566No Hit
CCCCCCACTGCTAAATTTGACTGG200590.21702856848566446No Hit
TGAGGTAGTAGTTTGTACAGTT198850.21514597359476736No Hit
CTCCCACTGCTTCACTTGACTAGCCT175250.18961192794811654No Hit
AACGGAATCCCAAAAGCAGCTG170940.1849487187643426No Hit
ACCCCCCACTGCTAAATTTGACTG165560.1791278219177756No Hit
TCGTACGACTCTTAGCGG164600.178089148874522No Hit
TCGTTTCCCGGCCAATGCACCA154360.16700996974648372No Hit
TGTCAGTTTGTCAAATACCCCAA152930.16546277969247056No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC145190.15708847828123845No Hit
TGAGGTAGTAGGTTGTGTGGTT142790.1544917956731045No Hit
TGAGGTAGTAGTTTGTGCTGTT141020.15257674224960568No Hit
TTCAAGTAATCCAGGATAGGC138070.14938498654377433No Hit
ATCCCACCGCTGCCACCA136930.1481515623049107No Hit
TATTGCACTTGTCCCGGCCTGTT136300.1474699331202755No Hit
GCGTTCGCGCTTTCCCCTG131830.14263361176262598No Hit
TGTAAACATCCTACACTCTCAGCT131240.1419952606214597No Hit
TTCACAGTGGCTAAGTTCTGC127900.1383815439918066No Hit
TCCTGTCTGAGCGTCGCT124790.13501667611209967No Hit
ATCCCGGACGAGCCCCCA121240.1311757497542348No Hit
GTCAGGATGGCCGAGTGGTCTAAGG121180.13111083268903145No Hit
CGCGACCTCAGATCAGACGTGGCG120730.13062395470000635No Hit
ACCACAGGGTAGAACCACGGAC117870.12752957459198003No Hit
AACATTCAACGCTGTCGGTGA116990.12657745763566422No Hit
AGCTCGGTCTGAGGCCCCTCAGT116920.12650172105959365No Hit
TATTGCACTTGTCCCGGCCTGTAG114600.12399159453839748No Hit
CACTAGATTGTGAGCTCCTGGA114250.1236129116580446No Hit
TCCCACTGCTTCACTTGACTAG109200.11814905867009602No Hit
CGCGACCTCAGATCAGACGTGG108750.1176621806810709No Hit
TTCACAGTGGCTAAGTTCCGC107700.11652613204001228No Hit
GGAATACCGGGTGCTGTAGGCTT107090.11586614187711157No Hit
TAGCTTATCAGACTGATGTTGACA104320.11286913736689028No Hit
TTCAAGTAATCCAGGATAGGCTT103720.11221996671485676No Hit
GGAATACCGGGTGCTGTAGGCTTT102410.1108026107912503No Hit
TTCACAGTGGCTAAGTTCCG100230.10844395742219527No Hit
TAGCAGCACATCATGGTTTACA99980.10817346965051465No Hit
TGTAAACATCCCCGACTGGAAGC99060.10717807465072995No Hit
ACTGGACTTGGAGTCAGAAGGCT97190.1051548261185589No Hit
AACATTCAACGCTGTCGGTGAGA97050.10500335296641775No Hit
CCCCCACTGCTAAATTTGACTGGCT94840.10261224106476105No Hit
TGTCAGTTTGTCAAATACCCCA94420.1021578216083376No Hit
CAAAGTGCTGTTCGTGCAGGT92800.10040506084784716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)