FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10512600
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG246963123.49210471244031No Hit
CAACGGAATCCCAAAAGCAGCTG8477508.064132564731846No Hit
CCCCCACTGCTAAATTTGACTG7375317.015685938778228No Hit
AAGCTGCCAGTTGAAGAACTGT2649822.5206133592070468No Hit
TAGCAGCACGTAAATATTGGCG2563002.4380267488537566No Hit
TTCAAGTAATCCAGGATAGGCT2378452.262475505583776No Hit
AACATTCAACGCTGTCGGTGAGT1929251.8351787378954778No Hit
TAGCTTATCAGACTGATGTTGACT1682351.6003177139813176No Hit
TAGCTTATCAGACTGATGTTGAC1572631.4959477198790023No Hit
AACATTCAACGCTGTCGGTGAG1433281.3633925004280578No Hit
TATTGCACTTGTCCCGGCCTGTAT1366831.3001826379772845No Hit
TATTGCACTTGTCCCGGCCTGTAA1250731.189743736088123No Hit
TAGCTTATCAGACTGATGTTGA1137751.0822727013298328No Hit
TGAGGTAGTAGGTTGTATAGTT915440.8708026558605864No Hit
TGTAAACATCCTTGACTGGAAGCT801010.761952323878013No Hit
TATTGCACTTGTCCCGGCCTGT781450.7433460799421646No Hit
TCGTACGACTCTTAGCGG674900.641991514943972No Hit
CATAAAGTAGAAAGCACTACT621280.5909860548294428No Hit
TATTGCACTTGTCCCGGCCTGTA606910.5773167437170633No Hit
AGCAGCATTGTACAGGGCTATGA586940.5583204916005555No Hit
CAACGGAATCCCAAAAGCAGCTGT542870.5163993683769953No Hit
TGAGGTAGTAGATTGTATAGTT510190.4853128626600461No Hit
TGTAAACATCCCCGACTGGAAGCT471790.44878526720316575No Hit
CCCATAAAGTAGAAAGCACTAC460740.4382740711146624No Hit
TTCAAGTAATTCAGGATAGGTT450960.42897094914673817No Hit
ACTGGACTTGGAGTCAGAAGGC407520.38764910678614234No Hit
CAACGGAATCCCAAAAGCAGCT366950.3490573216901623No Hit
ACCCCCCACTGCTAAATTTGACTG336920.32049160055552384No Hit
CCCATAAAGTAGAAAGCACT330450.3143370812168255No Hit
CATTGCACTTGTCTCGGTCTGA307300.2923158876015448No Hit
TTCTCACTACTGCACTTGACTAG303970.2891482601830185No Hit
CTTCTCACTACTGCACTTGACTAG280790.26709852938378703No Hit
CCCCCCACTGCTAAATTTGACTGT274020.2606586382055819No Hit
TGTAAACATCCTACACTCTCAGCT269700.25654928371668284No Hit
CACCCGGCTGTGTGCACATGTGC266190.25321043319445236No Hit
TAGCTTATCAGACTGATGTTGACA243930.2320358427030421No Hit
CCCCCCACTGCTAAATTTGACTGG239720.22803112455529556No Hit
TGAGGTAGTAGTTTGTACAGTT237090.22552936476228527No Hit
AACGGAATCCCAAAAGCAGCTG219500.20879706257253203No Hit
CCCCCCACTGCTAAATTTGACTGTA212740.20236668378897704No Hit
CAACACCAGTCGATGGGCTGT209770.19954150257785896No Hit
CTCCCACTGCTTCACTTGACTAG209360.19915149439719954No Hit
CCCCCACTGCTAAATTTGACTGGCTT202630.19274965279759526No Hit
CAAAGAATTCTCCTTTTGGGCT198620.18893518254285332No Hit
GACCCGCGGGCGCTCTCCAGTCCT189130.17990792001978578No Hit
CCCCCCACTGCTAAATTTGACTGA174790.16626714609135704No Hit
TATTGCACTTGTCCCGGCCTGTT169200.1609497174818789No Hit
TACGCCTGTCTGAGCGTCGCT161920.154024694176512No Hit
TAGCAGCACGTAAATATTGGC157540.14985826531971158No Hit
CACTAGATTGTGAGCTCCTGGA157110.14944923234975171No Hit
TCGTTTCCCGGCCAATGCACCA155800.14820310865057168No Hit
CGCCTGTCTGAGCGTCGCT154980.14742309228925288No Hit
TATTGCACTTGTCCCGGCCTGTAG153160.14569183646291117No Hit
CACTAGATTGTGAGCTCCTGGAA149230.1419534653653711No Hit
AGCTCGGTCTGAGGCCCCTCAGT148700.14144930844890893No Hit
CCCCCCACTGCTAAATTTGACTGGCT147140.13996537488347316No Hit
GCCTGTCTGAGCGTCGCT146230.1390997469703023No Hit
AGCAGCATTGTACAGGGCTATGT145310.1382246066624812No Hit
TTCAAGTAATCCAGGATAGGCTT139870.1330498639727565No Hit
TGTAAACATCCCCGACTGGAAGC138740.1319749633772806No Hit
AACATTCAACGCTGTCGGTGAGTT132260.12581093164393203No Hit
TGAGGTAGTAGTTTGTGCTGTT131590.12517360120236667No Hit
CAAAGAATTCTCCTTTTGGGCTT130490.12412723779084148No Hit
TATTGCACTTGTCCCGGCCTGTTT129470.1231569735365181No Hit
TCGTTTCCCGGGCGGCGCACCA124310.11824857789699979No Hit
CTACGCCTGTCTGAGCGTCGCT119820.11397751269904685No Hit
TGTCAGTTTGTCAAATACCCCA116700.11100964556817533No Hit
TGAGGTAGTAGGTTGTGTGGTT116140.1104769514677625No Hit
ACTGGACTTGGAGTCAGAAGGCT115510.10987767060479806No Hit
CCCATAAAGTAGAAAGCACTA112360.10688126628997585No Hit
TATTGCACTTGTCCCGGCCTGTAAT111880.10642467134676482No Hit
ACTGGACTTGGAGTCAGAAGGCA111090.10567319216939672No Hit
TTCAAGTAATCCAGGATAGGC107730.1024770275669197No Hit
TGTCAGTTTGTCAAATACCCCAA105400.10026063961341629No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)