FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7208152
Sequences flagged as poor quality0
Sequence length18-26
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG105710114.665353893758068No Hit
GGAATACCGGGTGCTGTAGGCTTT5382537.467281489069598No Hit
CAACGGAATCCCAAAAGCAGCTG4405146.111330615669592No Hit
GGAATACCGGGTGCTGTAGGCTT3643875.055206937922508No Hit
CCCCCACTGCTAAATTTGACTG2844403.946087707362442No Hit
TGAATACCGGGTGCTGTAGGCTTT2136612.9641578035535323No Hit
AAGCTGCCAGTTGAAGAACTGT1986882.756434658980554No Hit
TGAATACCGGGTGCTGTAGGCTT1446742.007088640750084No Hit
AACATTCAACGCTGTCGGTGAGT1089681.511732826943716No Hit
TTCAAGTAATCCAGGATAGGCT924761.2829363198778272No Hit
TATTGCACTTGTCCCGGCCTGTAA765611.0621446384593443No Hit
TAGCTTATCAGACTGATGTTGACT719200.997759203745981No Hit
GGAATACCGGGTGCTGTAGGCTTA718900.9973430083050412No Hit
TATTGCACTTGTCCCGGCCTGTAT714580.9913497939555104No Hit
AACATTCAACGCTGTCGGTGAG709250.9839553882881493No Hit
TAGCTTATCAGACTGATGTTGAC648890.9002168655710923No Hit
ACTGGACTTGGAGTCAGAAGGC590540.819266852308331No Hit
TGTAAACATCCTTGACTGGAAGCT544170.7549369103204261No Hit
TGTAAACATCCCCGACTGGAAGCT524790.7280506848357249No Hit
GGAATACCGGGTGCTGTAGGCT476440.6609738529376183No Hit
TATTGCACTTGTCCCGGCCTGT446460.619382055206383No Hit
TAGCTTATCAGACTGATGTTGA421700.5850320581474974No Hit
TCGTACGACTCTTAGCGG393160.5454379985327723No Hit
TAGCAGCACGTAAATATTGGCG327220.4539582406142379No Hit
AGCAGCATTGTACAGGGCTATGA316570.4391833024608804No Hit
TATTGCACTTGTCCCGGCCTGTA305070.4232291438915272No Hit
GCCTGTCTGAGCGTCGCT302230.41928916038396524No Hit
CAACGGAATCCCAAAAGCAGCTGT301500.4182764181443454No Hit
TGAATACCGGGTGCTGTAGGCTTA287560.3989372033220165No Hit
CACCCGGCTGTGTGCACATGTGC286610.39761925109237434No Hit
CACTAGATTGTGAGCTCCTGGA224700.31173038526379576No Hit
ACCGGGTGCTGTAGGCTT204410.283581700274911No Hit
TGAGAACTGAATTCCATGGGTT200820.2786012281649998No Hit
TGAATACCGGGTGCTGTAGGCT189370.26271643550247No Hit
CACTAGATTGTGAGCTCCTGGAA188110.26096841465052345No Hit
TGAGGTAGTAGGTTGTATAGTT172090.23874357810434627No Hit
ACTGGACTTGGAGTCAGAAGGCT170440.23645450317917824No Hit
AGCTCGGTCTGAGGCCCCTCAGT169750.23549725366501703No Hit
ACCGGGTGCTGTAGGCTTT154240.21397994936843728No Hit
CGCCTGTCTGAGCGTCGCT153100.21239840669286664No Hit
GGAATACCGGGTGCTGTAGGCTA148760.2063774459806064No Hit
CGAATACCGGGTGCTGTAGGCTTT137170.19029842877897135No Hit
TGAGGTAGTAGTTTGTGCTGTT136760.1897296283430205No Hit
CCCCCCACTGCTAAATTTGACTGT135390.1878290024960628No Hit
CCCCCACTGCTAAATTTGACTGGCTT134820.18703823115827747No Hit
CCCCCCACTGCTAAATTTGACTGTA133670.18544281530134216No Hit
CAAAGAATTCTCCTTTTGGGCT129670.1798935427554802No Hit
ACCCCCCACTGCTAAATTTGACTG127680.17713277966391386No Hit
TGTAAACATCCCCGACTGGAAGC125210.1737061038668441No Hit
GGGAATACCGGGTGCTGTAGGCTTT124280.17241589799993118No Hit
ACCGGGTGCTGTAGGCTTA123590.17145864848577No Hit
CCCCCCACTGCTAAATTTGACTGG119330.165548673224427No Hit
TATTGCACTTGTCCCGGCCTGTAG115250.15988841522764782No Hit
CCCCCCACTGCTAAATTTGACTGGCT114520.158875672988028No Hit
TGAGGGGCAGAGAGCGAGACTTT112540.15612878307782632No Hit
GGGAATACCGGGTGCTGTAGGCTT112240.15571258763688667No Hit
CAACACCAGTCGATGGGCTGT109010.15123155005610314No Hit
CTCCCACTGCTTCACTTGACTAG107580.1492476851209575No Hit
AACGGAATCCCAAAAGCAGCTG105470.1463204438530153No Hit
CCCCCCACTGCTAAATTTGACTGA105150.14587650204934635No Hit
TCCTGTCTGAGCGTCGCT105020.14569615069160582No Hit
ACCACAGGGTAGAACCACGGAC101700.1410902544785404No Hit
GACCCGCGGGCGCTCTCCAGTCCT101600.14095152266489386No Hit
ACTGGACTTGGAGTCAGAAGG99860.1385375891074439No Hit
TTCTCACTACTGCACTTGACTAG96830.1343340151539535No Hit
CTTCTCACTACTGCACTTGACTAG95800.13290507747339403No Hit
CGAATACCGGGTGCTGTAGGCTT92950.12895122078446736No Hit
CAAAGAATTCTCCTTTTGGGCTT92250.12798009808894154No Hit
CCCATAAAGTAGAAAGCACTAC92030.1276748880989191No Hit
TAGCTTATCAGACTGATGTTGACA89410.12404011458137952No Hit
TGAGGTAGTAGGTTGTGTGGTT88220.12238920599898559No Hit
CATTGCACTTGTCTCGGTCTGA87520.12141808330345975No Hit
GAATACCGGGTGCTGTAGGCTTT86620.1201694969806408No Hit
TATTGCACTTGTCCCGGCCTGTT85090.1180469002318486No Hit
TTAATGCTAATCGTGATAGGGGTT84700.11750584615862705No Hit
ACTGGACTTGGAGTCAGAAGGCA82740.1147867026111547No Hit
TTCAAGTAATTCAGGATAGGTT81870.11357973583242974No Hit
GTCCGATGGTAGTGGGTTATCAGAAC80860.11217854451459958No Hit
CCCATAAAGTAGAAAGCACT80740.11201206633822372No Hit
CATAAAGTAGAAAGCACTACT79380.11012531367263065No Hit
CCCCCACAACCGCGCTTGACTAG73020.10130197032471014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)