FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6071031
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG142126123.410537682973455No Hit
CAACGGAATCCCAAAAGCAGCTG5103618.406496359514554No Hit
CCCCCACTGCTAAATTTGACTG3473365.721202873119903No Hit
AAGCTGCCAGTTGAAGAACTGT1200341.9771600573279893No Hit
AACATTCAACGCTGTCGGTGAGT1150171.8945217047977518No Hit
TTCAAGTAATCCAGGATAGGCT1119441.8439042726021329No Hit
TAGCAGCACGTAAATATTGGCG1117421.8405769959006963No Hit
TAGCTTATCAGACTGATGTTGACT883721.4556341418780434No Hit
TAGCTTATCAGACTGATGTTGAC844291.3906863595326724No Hit
AACATTCAACGCTGTCGGTGAG761621.2545150897763493No Hit
TATTGCACTTGTCCCGGCCTGTAA754831.243330827992807No Hit
TATTGCACTTGTCCCGGCCTGTAT748881.2335301862237238No Hit
TCGTACGACTCTTAGCGG540430.8901782909690298No Hit
TAGCTTATCAGACTGATGTTGA524300.8636094923580525No Hit
TATTGCACTTGTCCCGGCCTGT420690.6929465522412915No Hit
TGAGGTAGTAGGTTGTATAGTT402320.662688100258424No Hit
TGTAAACATCCTTGACTGGAAGCT366280.6033242129714047No Hit
CAACGGAATCCCAAAAGCAGCTGT332920.5483747323971827No Hit
AGCAGCATTGTACAGGGCTATGA324150.5339290805795589No Hit
TATTGCACTTGTCCCGGCCTGTA319750.5266815471704888No Hit
TGTAAACATCCCCGACTGGAAGCT246790.40650426591463623No Hit
ACCCCCCACTGCTAAATTTGACTG239060.39377166744824726No Hit
ACTGGACTTGGAGTCAGAAGGC230590.3798201656357874No Hit
CCCATAAAGTAGAAAGCACTAC226140.37249027389252337No Hit
TTCAAGTAATTCAGGATAGGTT216130.356002135386889No Hit
TGAGGTAGTAGATTGTATAGTT213380.3514724270062202No Hit
CATAAAGTAGAAAGCACTACT208000.3426106702469482No Hit
TTAATGCTAATCGTGATAGGGGTT189110.3114956915884633No Hit
CAACGGAATCCCAAAAGCAGCT187990.3096508649025182No Hit
CCCCCACTGCTAAATTTGACTGGCTT168420.27741581289899525No Hit
CCCCCCACTGCTAAATTTGACTGT166890.2748956478726595No Hit
CCCCCCACTGCTAAATTTGACTGTA164460.2708930328308322No Hit
GGAATACCGGGTGCTGTAGGCTTT160090.2636949144222785No Hit
TAGCTTATCAGACTGATGTTGACA158560.26117474939594276No Hit
CACCCGGCTGTGTGCACATGTGC147960.2437147825468195No Hit
CATTGCACTTGTCTCGGTCTGA143210.23589074079839156No Hit
CAAAGAATTCTCCTTTTGGGCT130370.21474112057737804No Hit
CCCCCCACTGCTAAATTTGACTGG126900.20902545218431595No Hit
TGTAAACATCCTACACTCTCAGCT112180.18477915859760888No Hit
CCCATAAAGTAGAAAGCACT111880.184285008592445No Hit
TTCTCACTACTGCACTTGACTAG110700.18234135190546713No Hit
CACTAGATTGTGAGCTCCTGGAA108280.1783552085304786No Hit
CTTCTCACTACTGCACTTGACTAG106270.17504440349588068No Hit
GGAATACCGGGTGCTGTAGGCTT105740.1741714051534245No Hit
CACTAGATTGTGAGCTCCTGGA105110.1731336901425804No Hit
GACCCGCGGGCGCTCTCCAGTCCT104330.17184890012915433No Hit
TCGTTTCCCGGCCAATGCACCA101990.1679945300888762No Hit
CCCCCCACTGCTAAATTTGACTGGCT101750.16759921008474507No Hit
CCCCCCACTGCTAAATTTGACTGA100050.16479902672214983No Hit
CGCCTGTCTGAGCGTCGCT96600.15911630166276536No Hit
TCGTTTCCCGGGCGGCGCACCA96250.1585397933234075No Hit
AACGGAATCCCAAAAGCAGCTG96070.15824330332030917No Hit
TATTGCACTTGTCCCGGCCTGTAG91840.15127578824749865No Hit
AGCTCGGTCTGAGGCCCCTCAGT91510.1507322232418184No Hit
TGAGGTAGTAGTTTGTACAGTT89860.14801439821341714No Hit
TATTGCACTTGTCCCGGCCTGTT88690.14608721319327805No Hit
GCCTGTCTGAGCGTCGCT88540.1458401381906961No Hit
AACATTCAACGCTGTCGGTGAGTT85020.14004211146344006No Hit
CAACACCAGTCGATGGGCTGT83550.1376207764381371No Hit
TATTGCACTTGTCCCGGCCTGTAAT78490.12928611301770654No Hit
CCCGCCACTGCTAAATTTGACTG78430.1291872830166738No Hit
CGCCCCACTGCTAAATTTGACTG78380.12910492468247978No Hit
TGATGTAGTAGGTTGTATAGTT78340.12903903801512462No Hit
CAAAGAATTCTCCTTTTGGGCTT77820.12818251133950725No Hit
TAGCAGCACGTAAATATTGGC77360.12742481466492264No Hit
TACGCCTGTCTGAGCGTCGCT74170.12217035294334685No Hit
TATTGCACTTGTCCCGGCCTGTTT73540.12113263793250273No Hit
TTCAAGTAATCCAGGATAGGCTT72810.11993020625327067No Hit
AGCAGCATTGTACAGGGCTATGT71900.11843128457094025No Hit
TGAATACCGGGTGCTGTAGGCTTT69360.11424748119388617No Hit
CTCCCACTGCTTCACTTGACTAG69270.11409923619233701No Hit
CTACGCCTGTCTGAGCGTCGCT68850.1134074261851076No Hit
GCCCCCACTGCTAAATTTGACTG67110.11054135615515717No Hit
CCCCCCACTGCTAAATTTGAC65660.10815296446353181No Hit
TATTGCACTTGTCCCGGCCTGTAAA65310.10757645612417396No Hit
ACTGGACTTGGAGTCAGAAGGCA64820.10676934444907298No Hit
CCCCCCACTGCTAAATTTGACT62700.10327735107924832No Hit
TGTAAACATCCCCGACTGGAAGC62180.10242082440363096No Hit
TGAGGTAGTAGTTTGTGCTGTT61780.10176195773007911No Hit
AACCGGGCGGAAACACCA61640.10153135439433598No Hit
CTACGGGGATGATTTTACGAACTGAA61220.10083954438710659No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)