FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7794516
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG138946917.826238345010776No Hit
CAACGGAATCCCAAAAGCAGCTG7218309.260741783069019No Hit
CCCCCACTGCTAAATTTGACTG4686246.01222705810085No Hit
AACATTCAACGCTGTCGGTGAGT2153682.7630708564842257No Hit
TAGCAGCACGTAAATATTGGCG1919252.4623081150901482No Hit
TTCAAGTAATCCAGGATAGGCT1816112.329984312047085No Hit
AACATTCAACGCTGTCGGTGAG1580202.027322799773584No Hit
AAGCTGCCAGTTGAAGAACTGT1560222.001689392901368No Hit
TAGCTTATCAGACTGATGTTGAC1182411.516976807796661No Hit
TAGCTTATCAGACTGATGTTGACT998901.2815420482811248No Hit
CAACGGAATCCCAAAAGCAGCT826671.0605790019547077No Hit
TAGCTTATCAGACTGATGTTGA789581.0129942641724001No Hit
TATTGCACTTGTCCCGGCCTGTAA730690.9374411445175044No Hit
TGAGGTAGTAGGTTGTATAGTT691380.8870082504160617No Hit
TATTGCACTTGTCCCGGCCTGTAT659550.846171846975489No Hit
TATTGCACTTGTCCCGGCCTGT497920.6388081055962936No Hit
TCGTTTCCCGGCCAATGCACCA408280.5238041720614853No Hit
TATTGCACTTGTCCCGGCCTGTA396700.5089475728832938No Hit
TGTAAACATCCTTGACTGGAAGCT396660.5088962547514175No Hit
AGCAGCATTGTACAGGGCTATGA394260.505817166838839No Hit
CAACGGAATCCCAAAAGCAGCTGT377760.4846484374398615No Hit
CATAAAGTAGAAAGCACTACT369090.47352523235567157No Hit
CGCCTGTCTGAGCGTCGCT344320.4417464791912673No Hit
TGAGGTAGTAGATTGTATAGTT307690.39475189992553744No Hit
TGTAAACATCCCCGACTGGAAGCT296890.380896004318934No Hit
ACTGGACTTGGAGTCAGAAGGC271430.34823201337966336No Hit
CCCATAAAGTAGAAAGCACTAC251840.32309895829324103No Hit
TTCAAGTAATTCAGGATAGGTT208940.2680602618558997No Hit
CATTGCACTTGTCTCGGTCTGA202980.2604138602063297No Hit
TAGCTTATCAGACTGATGTTGACA199390.255808057870431No Hit
CTACGCCTGTCTGAGCGTCGCT185260.23767992778512484No Hit
CCCCCACTGCTAAATTTGACTGGCTT182760.23447254454285554No Hit
CAACACCAGTCGATGGGCTGT181810.23325373891079315No Hit
CACCCGGCTGTGTGCACATGTGC181140.232394160201865No Hit
TCGTACGACTCTTAGCGG176210.22606920044810994No Hit
GGAATACCGGGTGCTGTAGGCTTT170840.21917974124371545No Hit
ACCCCCCACTGCTAAATTTGACTG163540.20981418217628908No Hit
TTCTCACTACTGCACTTGACTAG158100.20283491624111105No Hit
CCCCCCACTGCTAAATTTGACTGG157950.2026424732465749No Hit
GTCAGGATGGCCGAGCGGTCTAAGG156450.2007180433012133No Hit
AACATTCAACGCTGTCGGTGAGTT155560.19957621486696547No Hit
CCCATAAAGTAGAAAGCACT153900.19744651239409863No Hit
AACGGAATCCCAAAAGCAGCTG151970.19497041253106673No Hit
CAACGGAATCCCAAAAGCAGCTGA151290.19409800428916946No Hit
TGAGGTAGTAGTTTGTACAGTT142370.18265406088075256No Hit
GGAATACCGGGTGCTGTAGGCTT137220.1760468514016778No Hit
TGTAAACATCCTACACTCTCAGCT133790.1716463215932843No Hit
TGATGTAGTAGGTTGTATAGTT133620.17142821953280998No Hit
TACGCCTGTCTGAGCGTCGCT123900.15895791348686691No Hit
CTTCTCACTACTGCACTTGACTAG123630.15861151609670182No Hit
TAGCAGCACGTAAATATTGGC116800.14984894507882207No Hit
GACCCGCGGGCGCTCTCCAGTCCT113230.1452688018088615No Hit
CAAAGAATTCTCCTTTTGGGCT112890.1448325976879129No Hit
TGTCAGTTTGTCAAATACCCCAA111850.14349832625912884No Hit
GCCTGTCTGAGCGTCGCT107970.13852046746712687No Hit
TGAGGTAGTAGGTTGTGTGGTT107770.13826387680774535No Hit
ACGCCTGTCTGAGCGTCGCT104610.13420974438951694No Hit
TAGCAGCACATCATGGTTTACA104090.1335426086751249No Hit
TTCACAGTGGCTAAGTTCTGC104080.13352977914215586No Hit
TATTGCACTTGTCCCGGCCTGTT102640.13168232639460872No Hit
CCCCCCACTGCTAAATTTGACTGTA101630.13038654356473192No Hit
CACTAGATTGTGAGCTCCTGGA98680.12660183133885414No Hit
ATCACATTGCCAGGGATTTCC96170.12338161856361575No Hit
TTCACAGTGGCTAAGTTCCG94850.12168812021169757No Hit
AATGCACCCGGGCAAGGATTCT92950.11925050894757289No Hit
TATTGCACTTGTCCCGGCCTGTAG92410.11855771416724271No Hit
TGAGGTAGTAGTTTGTGCTGTT91790.11776228312315994No Hit
CAACGGAATCCCAAAAGCAGCTT89870.1152990127930971No Hit
CCCCCCACTGCTAAATTTGACTGGCT87560.11233539067724024No Hit
CCCCCCACTGCTAAATTTGACTGT86740.11128336897377593No Hit
CACTAGATTGTGAGCTCCTGGAA86390.11083433531985823No Hit
TTCACAGTGGCTAAGTTCCGC84580.10851218985245524No Hit
CTCCCACTGCTTCACTTGACTAG83820.10753714534680538No Hit
ACTGGACTTGGAGTCAGAAGGCA83740.10743450908305276No Hit
ACTGGACTTGGAGTCAGAAGGCT82670.10606174905536149No Hit
TTCAAGTAATCCAGGATAGGC81290.10429127350562882No Hit
ACCACAGGGTAGAACCACGGAC80750.10359847872529865No Hit
AGCTCGGTCTGAGGCCCCTCAGT79960.10258494562074155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)