FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7011088
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5285887.5393148681060635No Hit
CCCCCCACTGCTAAATTTGACTG3289804.692281711483296No Hit
TAGCAGCACGTAAATATTGGCG2211153.153790110750286No Hit
AACATTCAACGCTGTCGGTGAGT2171083.096637782894752No Hit
AAGCTGCCAGTTGAAGAACTGT2050432.924553221982095No Hit
TTCAAGTAATCCAGGATAGGCT1967392.8061122610356626No Hit
TAGCTTATCAGACTGATGTTGACT1878392.679170479674481No Hit
TATTGCACTTGTCCCGGCCTGTAA1759332.509353755080524No Hit
TAGCTTATCAGACTGATGTTGAC1593392.272671516888677No Hit
TATTGCACTTGTCCCGGCCTGTAT1485472.1187439096471192No Hit
AACATTCAACGCTGTCGGTGAG1311311.8703373855812393No Hit
TAGCTTATCAGACTGATGTTGA1010881.4418304263189965No Hit
TGAGGTAGTAGGTTGTATAGTT953171.3595179521352463No Hit
CCCCCACTGCTAAATTTGACTG949551.3543547021517917No Hit
TGTAAACATCCTTGACTGGAAGCT785021.1196835640916218No Hit
TATTGCACTTGTCCCGGCCTGT767601.0948372064364333No Hit
TGAGGTAGTAGATTGTATAGTT645370.9204990723265775No Hit
TATTGCACTTGTCCCGGCCTGTA569290.8119852439450197No Hit
CATAAAGTAGAAAGCACTACT541420.7722339243210183No Hit
TGTAAACATCCCCGACTGGAAGCT511480.729530138546257No Hit
AGCAGCATTGTACAGGGCTATGA482920.688794663538669No Hit
ACTGGACTTGGAGTCAGAAGGC477380.6808928942269731No Hit
CCCATAAAGTAGAAAGCACTAC408890.5832047750648687No Hit
TTCAAGTAATTCAGGATAGGTT403430.5754171107251826No Hit
CAACGGAATCCCAAAAGCAGCTGT362930.5176514686450947No Hit
CTCCCACTGCTTCACTTGACTAG302860.43197289778704817No Hit
TGAGGTAGTAGTTTGTACAGTT268670.3832072853742529No Hit
GCCTGTCTGAGCGTCGCT267420.3814243951865959No Hit
CATTGCACTTGTCTCGGTCTGA228540.3259693787897114No Hit
TAGCTTATCAGACTGATGTTGACA227170.32401533114403924No Hit
ACCGGGTGCTGTAGGCTTA226300.32277443957343No Hit
CACCCGGCTGTGTGCACATGTGC225990.322332282806891No Hit
CCCATAAAGTAGAAAGCACT217620.31039405011033955No Hit
CAACACCAGTCGATGGGCTGT200390.2858186917636749No Hit
TATTGCACTTGTCCCGGCCTGTAG200080.28537653499713594No Hit
TGTCAGTTTGTCAAATACCCCAA198860.2836364341739827No Hit
AACATTCAACGCTGTCGGTGAGTT190440.2716268858699249No Hit
CGCCTGTCTGAGCGTCGCT187140.26692005577451033No Hit
TGTAAACATCCTACACTCTCAGCT177770.2535555109278332No Hit
CACTAGATTGTGAGCTCCTGGAA172320.24578210970964848No Hit
CCCCCACTGCTAAATTTGACTGGCTT167840.23939223127708567No Hit
CACTAGATTGTGAGCTCCTGGA167610.23906417948255676No Hit
CAAAGAATTCTCCTTTTGGGCT162170.23130504138587335No Hit
TATTGCACTTGTCCCGGCCTGTAAT162150.23127651514287081No Hit
TCGTACGACTCTTAGCGG160860.22943657246920876No Hit
CTCCCACTGCTTCACTTGACTAGCCT158540.22612752828091734No Hit
ACTGGACTTGGAGTCAGAAGGCA153450.21886759943677783No Hit
TATTGCACTTGTCCCGGCCTGTT150880.21520197721095496No Hit
TTCTCACTACTGCACTTGACTAG146100.20838420513335448No Hit
TGAGGTAGTAGTTTGTGCTGTT145690.207799417151803No Hit
CCCCCCACTGCTAAATTTGACTGGCT142890.20380574313145122No Hit
AGCTCGGTCTGAGGCCCCTCAGT140680.20065359327967355No Hit
ACCGGGTGCTGTAGGCTT139060.19834296759647005No Hit
ATCCCGGACGAGCCCCCA134630.19202440477141353No Hit
CAAAGAATTCTCCTTTTGGGCTT132440.18890078116263836No Hit
CGCGACCTCAGATCAGACGTGGCG128540.18333816377714843No Hit
TTCAAGTAATCCAGGATAGGCTT126340.18020027704687203No Hit
TATTGCACTTGTCCCGGCCTGTAAA125290.17870264928924012No Hit
ACTGGACTTGGAGTCAGAAGGCT124870.17810359818618737No Hit
ACCGGGTGCTGTAGGCTTT124240.17720502153160822No Hit
TATTGCACTTGTCCCGGCCTGTTT123430.17604970869000647No Hit
AACGGAATCCCAAAAGCAGCTG120610.17202750842665218No Hit
TAGCAGCACGTAAATATTGGC117070.16697836341520747No Hit
CTTCTCACTACTGCACTTGACTAG116750.16652194352716726No Hit
TGTCAGTTTGTCAAATACCCCA116590.16629373358314714No Hit
GGAATACCGGGTGCTGTAGGCTTT116320.16590862930261324No Hit
TGTAAACATCCCCGACTGGAAGC114530.16335553055388835No Hit
CCCCCCACTGCTAAATTTGACTGG98090.13990695880582302No Hit
ACTGGACTTGGAGTCAGAAGG97830.13953611764679033No Hit
AGCAGCATTGTACAGGGCTATGT97120.13852343602020115No Hit
ACCCCCCACTGCTAAATTTGACTG95960.13686891392605544No Hit
TTATAAAGCAATGAGACTGATT95330.13597033727147625No Hit
GGAATACCGGGTGCTGTAGGCTT92850.13243308313916471No Hit
TTCACAGTGGCTAAGTTCCG92780.13233324128865592No Hit
TTCACAGTGGCTAAGTTCCGC89760.1280257785952765No Hit
CAAAGTGCTGTTCGTGCAGGTAG89080.1270558863331911No Hit
TTCAAGTAATCCAGGATAGGC87860.1253157855100378No Hit
TCGTTTCCCGGGCGGCGCACCA86900.12394652584591721No Hit
CGCGACCTCAGATCAGACGTGG85250.12159311079820992No Hit
TGAGAACTGAATTCCATAGGCTGT82730.11799880417989334No Hit
TGAGGTAGTAGGTTGTATAGT81760.11661528139427148No Hit
CTACGCCTGTCTGAGCGTCGCT80630.1150035486646295No Hit
ACCACAGGGTAGAACCACGGAC79840.11387676206603026No Hit
TTCACAGTGGCTAAGTTCTGC75910.10827135531603654No Hit
TGAGGTAGTAGGTTGTGTGGTT75700.10797182976451017No Hit
TACGCCTGTCTGAGCGTCGCT72930.10402094510866217No Hit
CCCATAAAGTAGAAAGCACTA71970.10265168544454156No Hit
ATGCACCTGGGCAAGGATTCTGA71110.10142505699543353No Hit
AACCGGGCGGAAACACCA70820.10101142647189708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)