FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6662224
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5380928.076762354432994No Hit
CCCCCCACTGCTAAATTTGACTG2424583.6392952263388323No Hit
TTCAAGTAATCCAGGATAGGCT2012373.02056790645286No Hit
AACATTCAACGCTGTCGGTGAGT1988812.9852043401722907No Hit
TAGCAGCACGTAAATATTGGCG1987732.983583259884387No Hit
AAGCTGCCAGTTGAAGAACTGT1853372.7819088640670144No Hit
TAGCTTATCAGACTGATGTTGACT1347322.022327679165396No Hit
TATTGCACTTGTCCCGGCCTGTAA1328861.9946192142443726No Hit
TAGCTTATCAGACTGATGTTGAC1320261.9817106119518046No Hit
AACATTCAACGCTGTCGGTGAG1172961.760613272684917No Hit
TATTGCACTTGTCCCGGCCTGTAT1039851.5608151272007664No Hit
TGAGGTAGTAGGTTGTATAGTT977611.4673928706089738No Hit
TAGCTTATCAGACTGATGTTGA948491.4236837428462328No Hit
CCCCCACTGCTAAATTTGACTG799881.2006200932301287No Hit
TATTGCACTTGTCCCGGCCTGT672291.0091074692174864No Hit
TGTAAACATCCTTGACTGGAAGCT648070.9727532427609759No Hit
CATAAAGTAGAAAGCACTACT559280.8394794290915466No Hit
TGAGGTAGTAGATTGTATAGTT547780.8222179260259036No Hit
AGCAGCATTGTACAGGGCTATGA522650.7844977893268074No Hit
TATTGCACTTGTCCCGGCCTGTA472380.7090425059259491No Hit
ACTGGACTTGGAGTCAGAAGGC445360.668485478723021No Hit
TTCAAGTAATTCAGGATAGGTT424800.6376249132421846No Hit
TGTAAACATCCCCGACTGGAAGCT401150.6021262569376232No Hit
CAACGGAATCCCAAAAGCAGCTGT384990.5778700926297284No Hit
CCCATAAAGTAGAAAGCACTAC347930.5222430227503608No Hit
TTAATGCTAATCGTGATAGGGGTT287230.4311323065690977No Hit
TCGTACGACTCTTAGCGG259300.38920936912358395No Hit
TGAGGTAGTAGTTTGTACAGTT244710.3673097752342161No Hit
CACCCGGCTGTGTGCACATGTGC243480.36546354490632554No Hit
CTCCCACTGCTTCACTTGACTAG230460.3459205214354846No Hit
CAACGGAATCCCAAAAGCAGCT227720.34180778070506185No Hit
ACCGGGTGCTGTAGGCTTA214860.3225049172768733No Hit
CATTGCACTTGTCTCGGTCTGA205150.3079302046884043No Hit
ACCGGGTGCTGTAGGCTT198600.29809865294232074No Hit
TAGCTTATCAGACTGATGTTGACA192120.2883721712148976No Hit
CCCATAAAGTAGAAAGCACT182460.27387250863975754No Hit
CAACACCAGTCGATGGGCTGT177360.26621740728021154No Hit
CGCCTGTCTGAGCGTCGCT172830.2594178760726148No Hit
AACATTCAACGCTGTCGGTGAGTT171480.257391525712735No Hit
ACCGGGTGCTGTAGGCTTT168260.25255830485435493No Hit
TGTAAACATCCTACACTCTCAGCT156430.23480147170074137No Hit
CCCCCACTGCTAAATTTGACTGGCTT155790.2338408315301317No Hit
TGAGGTAGTAGTTTGTGCTGTT152600.229052640679749No Hit
ACTGGACTTGGAGTCAGAAGGCA148790.22333382966408816No Hit
CGCGACCTCAGATCAGACGTGGCG141390.21222642769141356No Hit
TTCTCACTACTGCACTTGACTAG137040.20569707653180078No Hit
TATTGCACTTGTCCCGGCCTGTAG135910.20400094623056805No Hit
CACTAGATTGTGAGCTCCTGGA134830.20237986594266422No Hit
CTCCCACTGCTTCACTTGACTAGCCT130760.19627079485769316No Hit
AACGGAATCCCAAAAGCAGCTG129320.19410935447382135No Hit
CGCGACCTCAGATCAGACGTGG127500.19137753398865004No Hit
TGTCAGTTTGTCAAATACCCCAA126150.1893511836287702No Hit
CAAAGAATTCTCCTTTTGGGCT124380.18669441315692778No Hit
TATTGCACTTGTCCCGGCCTGTT122470.18382750264776446No Hit
CACTAGATTGTGAGCTCCTGGAA121660.1826116924318366No Hit
TCGTTTCCCGGCCAATGCACCA115360.1731553907523974No Hit
TAGCAGCACGTAAATATTGGC114360.17165439048581976No Hit
TTCAAGTAATCCAGGATAGGCTT111640.16757166976072857No Hit
ACTGGACTTGGAGTCAGAAGGCT107400.16120742863043933No Hit
TATTGCACTTGTCCCGGCCTGTAAT106530.15990155839851677No Hit
AGCAGCATTGTACAGGGCTATGT106040.15916606826789373No Hit
CTTCTCACTACTGCACTTGACTAG104830.15734985794533476No Hit
CAAAGAATTCTCCTTTTGGGCTT104560.1569445878733588No Hit
TTATAAAGCAATGAGACTGATT99620.14952964655646522No Hit
CCCCCCACTGCTAAATTTGACTGGCT99450.14927447651114703No Hit
TGTAAACATCCCCGACTGGAAGC96560.14493658574073762No Hit
GGAATACCGGGTGCTGTAGGCTTT96290.14453131566876165No Hit
CAACGGAATCCCAAAAGCAGCTGA93700.14064372497832556No Hit
TGAGGTAGTAGGTTGTGTGGTT93180.13986320483970516No Hit
TTCAAGTAATCCAGGATAGGC91600.13749162441851248No Hit
TTCACAGTGGCTAAGTTCCGC89350.1341143738187128No Hit
ACTGGACTTGGAGTCAGAAGG89340.134099363816047No Hit
CTACGCCTGTCTGAGCGTCGCT88910.13345393370141864No Hit
TTCACAGTGGCTAAGTTCCG88550.13291357360545067No Hit
GGAATACCGGGTGCTGTAGGCTT87660.13157768336819659No Hit
TATTGCACTTGTCCCGGCCTGTAAA85950.12901097291234878No Hit
TGAGGTAGTAGGTTGTATAGT85810.12880083287502792No Hit
TTCACAGTGGCTAAGTTCTGC83650.12555867229922021No Hit
CAAAGTGCTGTTCGTGCAGGTAG83090.12471811214993672No Hit
TGTCAGTTTGTCAAATACCCCA81960.12302198184870397No Hit
AGCTCGGTCTGAGGCCCCTCAGT81770.12273679179805423No Hit
TGAGAACTGAATTCCATGGGTT81590.12246661175007025No Hit
ACCACAGGGTAGAACCACGGAC81410.12219643170208627No Hit
TATTGCACTTGTCCCGGCCTGTTT80710.12114573151548191No Hit
CCCCCCACTGCTAAATTTGACTGG76740.11518676045716865No Hit
ACCCCCCACTGCTAAATTTGACTG76600.11497662041984778No Hit
TCGTTTCCCGGGCGGCGCACCA73920.1109539397054197No Hit
TGATGTAGTAGGTTGTATAGTT72890.1094079094308447No Hit
ATCCCGGACGAGCCCCCA72320.10855233927889545No Hit
TAGCAGCACGTAAATATTGGCGT71910.10793692916959861No Hit
AGCAGCATTGTACAGGGCTATG71540.10738155907096489No Hit
TACGCCTGTCTGAGCGTCGCT71390.10715640903097823No Hit
ATGCACCTGGGCAAGGATTCTGA71040.10663105893767608No Hit
TGAGAACTGAATTCCATAGGCTGT68040.10212805813794312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)