FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9923127
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7679797.739284199426249No Hit
CCCCCCACTGCTAAATTTGACTG4364164.397968503275227No Hit
TTCAAGTAATCCAGGATAGGCT3132383.1566460854527003No Hit
AACATTCAACGCTGTCGGTGAGT3013383.0367242100196843No Hit
AAGCTGCCAGTTGAAGAACTGT2947792.9706260939721925No Hit
TAGCAGCACGTAAATATTGGCG2811372.8331492683707467No Hit
TATTGCACTTGTCCCGGCCTGTAA1994292.009739470229495No Hit
AACATTCAACGCTGTCGGTGAG1757741.7713569522994113No Hit
TAGCTTATCAGACTGATGTTGACT1679321.6923294441359058No Hit
TATTGCACTTGTCCCGGCCTGTAT1544141.556102224631409No Hit
TAGCTTATCAGACTGATGTTGAC1471651.4830506553025071No Hit
CCCCCACTGCTAAATTTGACTG1414861.425820711555944No Hit
TGAGGTAGTAGGTTGTATAGTT1297661.3077127804572086No Hit
TAGCTTATCAGACTGATGTTGA1159941.168925883947671No Hit
GCCTGTCTGAGCGTCGCT1045911.0540125103709748No Hit
TATTGCACTTGTCCCGGCCTGT1010731.0185599761043067No Hit
CATAAAGTAGAAAGCACTACT831770.8382135994026882No Hit
TGTAAACATCCTTGACTGGAAGCT809090.8153579007907487No Hit
TGAGGTAGTAGATTGTATAGTT729800.7354536528656742No Hit
TTAATGCTAATCGTGATAGGGGTT649170.6541990241584129No Hit
TATTGCACTTGTCCCGGCCTGTA628240.6331068825381354No Hit
CAACGGAATCCCAAAAGCAGCTGT591400.5959814884965193No Hit
ACTGGACTTGGAGTCAGAAGGC558070.562393285906751No Hit
TGTAAACATCCCCGACTGGAAGCT552840.5571227698688125No Hit
CCCATAAAGTAGAAAGCACTAC546730.5509654366007812No Hit
AGCAGCATTGTACAGGGCTATGA539410.5435887296413722No Hit
TTCAAGTAATTCAGGATAGGTT522550.5265981177102742No Hit
ACCGGGTGCTGTAGGCTTA475550.47923401564849466No Hit
ACCGGGTGCTGTAGGCTT441690.4451117072269658No Hit
CTCCCACTGCTTCACTTGACTAG409310.4124808641469569No Hit
ACCGGGTGCTGTAGGCTTT368720.3715764194089222No Hit
TGAGAACTGAATTCCATGGGTT327180.32971461516112816No Hit
TGAGGTAGTAGTTTGTACAGTT325120.3276386566452289No Hit
CACCCGGCTGTGTGCACATGTGC320250.3227309294741466No Hit
CCCATAAAGTAGAAAGCACT311970.3143867855364544No Hit
CGCCTGTCTGAGCGTCGCT291920.29418146114626975No Hit
CATTGCACTTGTCTCGGTCTGA288770.2910070585612781No Hit
TCCTGTCTGAGCGTCGCT284430.2866334372219564No Hit
TGTAAACATCCTACACTCTCAGCT280650.2828241541199664No Hit
CAACACCAGTCGATGGGCTGT252860.25481886909237383No Hit
AACATTCAACGCTGTCGGTGAGTT236420.23825151083927473No Hit
TGAGAACTGAATTCCATAGGCTGT215590.21726014390423504No Hit
CCCCCACTGCTAAATTTGACTGGCTT212200.21384388207467261No Hit
TTCTCACTACTGCACTTGACTAG211140.21277567041115167No Hit
TATTGCACTTGTCCCGGCCTGTAG210560.21219117723677225No Hit
GGAATACCGGGTGCTGTAGGCTTT205920.207515231841737No Hit
CAAAGAATTCTCCTTTTGGGCT197410.19893930612799776No Hit
ATCCCGGACGAGCCCCCA197160.19868736941490317No Hit
TAGCTTATCAGACTGATGTTGACA197060.19858659472966536No Hit
TGAGGTAGTAGTTTGTGCTGTT191330.19281220526553777No Hit
TAGCAGCACATCATGGTTTACA190330.1918044584131595No Hit
CACTAGATTGTGAGCTCCTGGA182980.18439751904817905No Hit
CTACGCCTGTCTGAGCGTCGCT182070.1834804694125148No Hit
GGAATACCGGGTGCTGTAGGCTT178050.17942932706595413No Hit
ACTGGACTTGGAGTCAGAAGGCA175010.1763657766347241No Hit
TTCACAGTGGCTAAGTTCCGC174010.17535802978234583No Hit
AACGGAATCCCAAAAGCAGCTG173320.1746626844542048No Hit
CTCCCACTGCTTCACTTGACTAGCCT172980.1743200505243962No Hit
TATTGCACTTGTCCCGGCCTGTT172350.1736851700073979No Hit
CACTAGATTGTGAGCTCCTGGAA171870.17320145151825628No Hit
TATTGCACTTGTCCCGGCCTGTAAT157760.15898214343119865No Hit
TTCAAGTAATCCAGGATAGGC153780.15497131095873307No Hit
AGCTCGGTCTGAGGCCCCTCAGT153720.15491084614759038No Hit
TAGCAGCACGTAAATATTGGC152700.15388294435816452No Hit
CTTCTCACTACTGCACTTGACTAG152680.15386278942111697No Hit
TGAGGTAGTAGGTTGTGTGGTT151620.15279457775759597No Hit
CCCCCCACTGCTAAATTTGACTGGCT146350.1474837518455624No Hit
TTCAAGTAATCCAGGATAGGCTT144320.14543802573523446No Hit
ACTGGACTTGGAGTCAGAAGGCT144110.14522639889623504No Hit
TTCACAGTGGCTAAGTTCCG142460.14356361658981084No Hit
CAAAGAATTCTCCTTTTGGGCTT140690.14177990466110127No Hit
ACCCCCCACTGCTAAATTTGACTG138080.13914968537639397No Hit
TGTAAACATCCCCGACTGGAAGC136990.13805124130730162No Hit
TATTGCACTTGTCCCGGCCTGTAAA131640.13265979564707778No Hit
TTCACAGTGGCTAAGTTCTGC121940.12288465117900839No Hit
TGTAAACATCCTACACTCAGCT121480.12242108762691437No Hit
TCGTACGACTCTTAGCGG120330.12126217874667934No Hit
TGAGGTAGTAGGTTGTATAGT120110.12104047443915612No Hit
TATTGCACTTGTCCCGGCCTGTTT119910.12083892506868046No Hit
CCCCCCACTGCTAAATTTGACTGG119380.12030481923691998No Hit
ACTGGACTTGGAGTCAGAAGG114180.11506453560455289No Hit
TACGCCTGTCTGAGCGTCGCT113260.11413740850036486No Hit
TCAGGCTCAGTCCCCTCCCGAT112720.11359322520008057No Hit
TCCCACTGCTTCACTTGACTAG109090.10993510412594738No Hit
CGCGACCTCAGATCAGACGTGGCG107670.10850410359557024No Hit
CCCATAAAGTAGAAAGCACTA106080.10690178610028875No Hit
GAATACCGGGTGCTGTAGGCTTT105030.10584365190529155No Hit
GTCAGGATGGCCGAGCGGTCTAAGG104940.1057529546885775No Hit
ACCACAGGGTAGAACCACGGAC104820.10563202506629212No Hit
CAAAGTGCTGTTCGTGCAGGTAG103660.10446303871753329No Hit
ATCACATTGCCAGGGATTTCC101910.1026994817258713No Hit
AGCAGCATTGTACAGGGCTATGT101500.10228630551639618No Hit
GGGAATACCGGGTGCTGTAGGCTT101280.10206460120887297No Hit
TTATAAAGCAATGAGACTGATT99810.10058321333587689No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)