FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8423221
Sequences flagged as poor quality0
Sequence length18-26
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG5641686.697770365991822No Hit
CCCCCCACTGCTAAATTTGACTG3439074.082844318105865No Hit
TTCAAGTAATCCAGGATAGGCT2103202.4969070620371943No Hit
TAGCAGCACGTAAATATTGGCG1973582.343022936237812No Hit
AACATTCAACGCTGTCGGTGAGT1880612.232649481712518No Hit
AAGCTGCCAGTTGAAGAACTGT1634171.9400773172163No Hit
GGAATACCGGGTGCTGTAGGCTTT1538091.8260116884028093No Hit
AACATTCAACGCTGTCGGTGAG1389411.649499639152291No Hit
GGAATACCGGGTGCTGTAGGCTT1245291.4784011959320549No Hit
CCCCCACTGCTAAATTTGACTG1184441.4061604224797142No Hit
TAGCTTATCAGACTGATGTTGAC1068361.2683509075684944No Hit
TAGCTTATCAGACTGATGTTGACT966341.147233344583978No Hit
TATTGCACTTGTCCCGGCCTGTAA957451.1366791872135373No Hit
GCCTGTCTGAGCGTCGCT956731.1358244073140191No Hit
TAGCTTATCAGACTGATGTTGA828570.983673585199771No Hit
TATTGCACTTGTCCCGGCCTGTAT756560.8981837233048972No Hit
ACCGGGTGCTGTAGGCTT753470.8945152929027981No Hit
TGAGGTAGTAGGTTGTATAGTT704110.8359153820136026No Hit
TATTGCACTTGTCCCGGCCTGT682870.8106993749778144No Hit
ACCGGGTGCTGTAGGCTTA617640.7332586904700708No Hit
ACCGGGTGCTGTAGGCTTT557080.661362203366147No Hit
CATAAAGTAGAAAGCACTACT518650.6157383262293605No Hit
TGAATACCGGGTGCTGTAGGCTTT495880.5887059119070959No Hit
TTAATGCTAATCGTGATAGGGGTT495860.5886821680209982No Hit
TGTAAACATCCTTGACTGGAAGCT431030.5117163612352091No Hit
TATTGCACTTGTCCCGGCCTGTA404680.4804337913014511No Hit
TGAATACCGGGTGCTGTAGGCTT403330.4788310789898544No Hit
TGAGGTAGTAGATTGTATAGTT377920.4486644717026895No Hit
TTCAAGTAATTCAGGATAGGTT355600.4221662948176238No Hit
CAACGGAATCCCAAAAGCAGCTGT351690.4175243650855177No Hit
AGCAGCATTGTACAGGGCTATGA327890.3892691406292201No Hit
TCCTGTCTGAGCGTCGCT287210.34097407630643906No Hit
CTCCCACTGCTTCACTTGACTAG281420.33410022128114647No Hit
ACTGGACTTGGAGTCAGAAGGC280710.3332573133246771No Hit
TACGCCTGTCTGAGCGTCGCT272620.32365291139814567No Hit
CCCATAAAGTAGAAAGCACTAC255600.3034468643289782No Hit
GGAATACCGGGTGCTGTAGGCTTA255280.30306696215141454No Hit
CATTGCACTTGTCTCGGTCTGA253070.30044326273761546No Hit
TGTAAACATCCCCGACTGGAAGCT250350.2972140942283243No Hit
CTACGCCTGTCTGAGCGTCGCT239480.2843092921342085No Hit
TCGTACGACTCTTAGCGG235950.2801184962379593No Hit
CAACGGAATCCCAAAAGCAGCT228040.2707277892863074No Hit
CAACACCAGTCGATGGGCTGT211570.25117469908482754No Hit
CCCATAAAGTAGAAAGCACT208250.2472332139926045No Hit
CACCCGGCTGTGTGCACATGTGC202470.24037123091036078No Hit
CGCCTGTCTGAGCGTCGCT193300.22948465913455196No Hit
GGAATACCGGGTGCTGTAGGCT191430.2272646057844143No Hit
AACCGGGCGGAAACACCA188860.2242135164208561No Hit
ATCCCGGACGAGCCCCCA178730.21218723811235632No Hit
TGAGGTAGTAGTTTGTACAGTT176570.20962289841380158No Hit
AACATTCAACGCTGTCGGTGAGTT156040.1852497993344826No Hit
TTCTCACTACTGCACTTGACTAG155610.18473930578338144No Hit
TTCACAGTGGCTAAGTTCCG154350.1832434409592245No Hit
CGTTCGCGCTTTCCCCTG149920.1779841701885775No Hit
TAGCTTATCAGACTGATGTTGACA143670.17056420578303716No Hit
CCCCCACTGCTAAATTTGACTGGCTT141800.1683441524328995No Hit
CTCCCACTGCTTCACTTGACTAGCCT133130.1580511778095339No Hit
AACGGAATCCCAAAAGCAGCTG125610.14912347663678774No Hit
TGTAAACATCCTACACTCTCAGCT125210.14864859891483317No Hit
CAAAGAATTCTCCTTTTGGGCT122660.1456212534373727No Hit
TGAGGTAGTAGTTTGTGCTGTT121730.1445171627338283No Hit
TATTGCACTTGTCCCGGCCTGTT118310.14045695821111662No Hit
TGAGAACTGAATTCCATGGGTT115090.13663419254938222No Hit
TAGCAGCACGTAAATATTGGC111350.1321940858491069No Hit
CTGGACGCGAGCCGGGCCCT107900.12809826549724862No Hit
CACTAGATTGTGAGCTCCTGGA107660.12781333886407586No Hit
CTTCTCACTACTGCACTTGACTAG103740.12315953718892096No Hit
TATTGCACTTGTCCCGGCCTGTAG103710.12312392135977437No Hit
ACCTGGTGCTGTAGGCTT103480.12285086666965048No Hit
AGCTCGGTCTGAGGCCCCTCAGT102370.12153308099122652No Hit
GTAATACCGGGTGCTGTAGGCTTT99540.11817332110839783No Hit
CAACGGAATCCCAAAAGCAGCTGA97140.11532405477667036No Hit
TGAGGTAGTAGGTTGTGTGGTT95770.1136975985789759No Hit
ACTGGACTTGGAGTCAGAAGGCA95200.11302089782519063No Hit
TTCAAGTAATCCAGGATAGGC94900.11266473953372468No Hit
ACCCCCCACTGCTAAATTTGACTG92050.1092812357647983No Hit
TTCACAGTGGCTAAGTTCCGC91520.10865202278320847No Hit
CGCGACCTCAGATCAGACGTGG91300.10839084003613346No Hit
CACCCGTAGAACCGACCTTGCG90730.10771413928234816No Hit
CACTAGATTGTGAGCTCCTGGAA89600.10637260971782647No Hit
TGAGAACTGAATTCCATAGGCTGT88420.10497172043806045No Hit
TTCACAGTGGCTAAGTTCTGC87540.10392698944976038No Hit
CGCGACCTCAGATCAGACGTGGCG85980.1020749663341375No Hit
TTCACAGTGGCTAAGTTCTG84760.10062658928217602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)