FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8520992
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG7881249.249204787423812No Hit
CCCCCCACTGCTAAATTTGACTG4637755.442734836507299No Hit
TTCAAGTAATCCAGGATAGGCT2626973.082939169523924No Hit
TAGCAGCACGTAAATATTGGCG2451242.876707312951356No Hit
AACATTCAACGCTGTCGGTGAGT2312762.714191023768125No Hit
AAGCTGCCAGTTGAAGAACTGT1907702.2388238364735No Hit
AACATTCAACGCTGTCGGTGAG1646461.9322398143314767No Hit
TATTGCACTTGTCCCGGCCTGTAA1624091.9059870024522967No Hit
TAGCTTATCAGACTGATGTTGACT1620521.9017973494165938No Hit
TAGCTTATCAGACTGATGTTGAC1586461.8618254775969747No Hit
CCCCCACTGCTAAATTTGACTG1481431.7385651811432283No Hit
TATTGCACTTGTCCCGGCCTGTAT1241521.4570134557103211No Hit
TAGCTTATCAGACTGATGTTGA1129561.3256203033637397No Hit
TGAGGTAGTAGGTTGTATAGTT865141.0153043213747883No Hit
TGTAAACATCCTTGACTGGAAGCT730030.8567429707714782No Hit
TATTGCACTTGTCCCGGCCTGT721550.8467910778463353No Hit
TATTGCACTTGTCCCGGCCTGTA597340.7010216650831265No Hit
CATAAAGTAGAAAGCACTACT534130.6268401613333283No Hit
AGCAGCATTGTACAGGGCTATGA526040.6173459615969595No Hit
TGAGGTAGTAGATTGTATAGTT505620.5933816156616507No Hit
CAACGGAATCCCAAAAGCAGCTGT488450.5732313796327939No Hit
TGTAAACATCCCCGACTGGAAGCT434670.5101166624731017No Hit
TTCAAGTAATTCAGGATAGGTT434360.5097528550666401No Hit
CTCCCACTGCTTCACTTGACTAG420600.4936045005088609No Hit
CAACGGAATCCCAAAAGCAGCT398380.4675277244715169No Hit
ACTGGACTTGGAGTCAGAAGGC388280.45567464445454237No Hit
CCCCCACTGCTAAATTTGACTGGCTT349730.41043343310262465No Hit
CCCATAAAGTAGAAAGCACTAC323070.3791459961469275No Hit
CACCCGGCTGTGTGCACATGTGC275480.32329569139367814No Hit
TAGCTTATCAGACTGATGTTGACA272970.3203500249736181No Hit
CATTGCACTTGTCTCGGTCTGA256040.3004814462916994No Hit
CTCCCACTGCTTCACTTGACTAGCCT253610.29762966565395205No Hit
ACCGGGTGCTGTAGGCTT241330.2832181980689572No Hit
ACCGGGTGCTGTAGGCTTA233560.2740995414618392No Hit
TTCTCACTACTGCACTTGACTAG229100.26886540909790785No Hit
TGAGGTAGTAGTTTGTACAGTT223170.26190612548398123No Hit
TACGCCTGTCTGAGCGTCGCT221860.26036874579861125No Hit
CTACGCCTGTCTGAGCGTCGCT209550.24592207104524919No Hit
CAACACCAGTCGATGGGCTGT201890.23693250738881108No Hit
AACATTCAACGCTGTCGGTGAGTT200630.23545380631738652No Hit
CCCATAAAGTAGAAAGCACT199850.234538419939838No Hit
CAAAGAATTCTCCTTTTGGGCT197130.2313463033412072No Hit
TGTAAACATCCTACACTCTCAGCT185540.2177446006286592No Hit
GCCTGTCTGAGCGTCGCT179010.21008117364738754No Hit
CGCCTGTCTGAGCGTCGCT175470.20592672778005192No Hit
ACCGGGTGCTGTAGGCTTT174950.20531647019501956No Hit
TATTGCACTTGTCCCGGCCTGTAG174230.2044714981542055No Hit
CAACGGAATCCCAAAAGCAGCTGA172090.20196005347734158No Hit
CTTCTCACTACTGCACTTGACTAG168560.19781734333279505No Hit
TCGTTTCCCGGCCAATGCACCA161750.18982531611342904No Hit
TGATGTAGTAGGTTGTATAGTT160980.1889216654586696No Hit
CCCCCCACTGCTAAATTTGACTGGCT158840.1864102207818057No Hit
TTCACAGTGGCTAAGTTCCG155480.18246701792467354No Hit
AACGGAATCCCAAAAGCAGCTG154420.1812230313090307No Hit
TATTGCACTTGTCCCGGCCTGTT152090.17848860789917417No Hit
TAGCAGCACGTAAATATTGGC151600.1779135574825091No Hit
GTCAGGATGGCCGAGCGGTCTAAGG141180.16568493433628387No Hit
TGAGGTAGTAGTTTGTGCTGTT140580.16498079096893883No Hit
AGCTCGGTCTGAGGCCCCTCAGT128780.15113263807782004No Hit
CACTAGATTGTGAGCTCCTGGAA128410.15069841633462397No Hit
ACCCCCCACTGCTAAATTTGACTG127400.1495131083329265No Hit
ACTGGACTTGGAGTCAGAAGGCA126790.14879722924279237No Hit
TTCACAGTGGCTAAGTTCCGC126050.1479287857564002No Hit
CACTAGATTGTGAGCTCCTGGA123840.1453351910200127No Hit
TCGTACGACTCTTAGCGG122650.14393864000811174No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC119960.1407817305778482No Hit
ACTGGACTTGGAGTCAGAAGGCT118860.139490801071049No Hit
TATTGCACTTGTCCCGGCCTGTAAA116360.13655687037377806No Hit
TATTGCACTTGTCCCGGCCTGTAAT113860.13362293967650715No Hit
TTCACAGTGGCTAAGTTCTGC113730.13347037528024905No Hit
TTCAAGTAATCCAGGATAGGCTT112490.1320151456544027No Hit
TTCAAGTAATCCAGGATAGGC111920.1313462094554249No Hit
CCCCCCACTGCTAAATTTGACTGG111240.1305481803057672No Hit
AGCAGCATTGTACAGGGCTATGT109790.12884650050135008No Hit
CAAAGAATTCTCCTTTTGGGCTT108970.1278841712326452No Hit
TGTAAACATCCCCGACTGGAAGC107090.12567785534829748No Hit
TGTCAGTTTGTCAAATACCCCAA106740.12526710505067956No Hit
TATTGCACTTGTCCCGGCCTGTTT105690.12403485415782575No Hit
TAGCAGCACATCATGGTTTACA102460.12024421569695172No Hit
ATGCACCTGGGCAAGGATTCTGA101950.11964569383470845No Hit
TTAATGCTAATCGTGATAGGGGTT97940.11493966899628588No Hit
CGTTCGCGCTTTCCCCTG97390.11429420424288628No Hit
TGATGTAGTAGATTGTATAGTT96150.1128389746170399No Hit
TCCCACTGCTTCACTTGACTAG94340.11071480879221575No Hit
ACTGGACTTGGAGTCAGAAGG93930.1102336441578633No Hit
TGAGGTAGTAGGTTGTGTGGTT93930.1102336441578633No Hit
TTCACAGTGGCTAAGTTCTG91900.10785129243167932No Hit
ACTCCCACTGCTTCACTTGACTAG89790.10537505492318265No Hit
TAGCAGCACGTAAATATTGGCGT86860.10193648814598112No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)