FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7878827
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG89386011.345089821111696No Hit
CCCCCCACTGCTAAATTTGACTG5990197.602895710237069No Hit
AACATTCAACGCTGTCGGTGAGT3437894.363454103002897No Hit
AAGCTGCCAGTTGAAGAACTGT3097483.931397402176745No Hit
TAGCAGCACGTAAATATTGGCG2849503.616655118839391No Hit
TTCAAGTAATCCAGGATAGGCT2527873.2084344535043092No Hit
AACATTCAACGCTGTCGGTGAG2332682.960694529782162No Hit
CCCCCACTGCTAAATTTGACTG2235532.837389372808922No Hit
TATTGCACTTGTCCCGGCCTGTAA1325881.6828393363631413No Hit
TAGCTTATCAGACTGATGTTGAC1283131.628579990397048No Hit
TAGCTTATCAGACTGATGTTGACT1259541.5986389852195002No Hit
TATTGCACTTGTCCCGGCCTGTAT1103741.400893813254181No Hit
TGAGGTAGTAGGTTGTATAGTT982131.2465434258170665No Hit
TAGCTTATCAGACTGATGTTGA893331.1338362931436368No Hit
TATTGCACTTGTCCCGGCCTGT770250.9776201457399686No Hit
CATAAAGTAGAAAGCACTACT768650.9755893865926997No Hit
TGTAAACATCCTTGACTGGAAGCT703960.8934832558196798No Hit
TGAGGTAGTAGATTGTATAGTT532810.6762554882852485No Hit
CAACGGAATCCCAAAAGCAGCTGT523580.6645405464544405No Hit
TATTGCACTTGTCCCGGCCTGTA512630.6506425385403182No Hit
AGCAGCATTGTACAGGGCTATGA503920.6395875934323726No Hit
ACTGGACTTGGAGTCAGAAGGC488690.6202573047993057No Hit
TGTAAACATCCCCGACTGGAAGCT447180.5675717971723455No Hit
TTCAAGTAATTCAGGATAGGTT433930.5507545729840241No Hit
CCCATAAAGTAGAAAGCACTAC416930.529177757044291No Hit
CCCATAAAGTAGAAAGCACT412120.5230727873578135No Hit
CATTGCACTTGTCTCGGTCTGA298090.37834312138088577No Hit
CGCCTGTCTGAGCGTCGCT289800.36782125054909826No Hit
TGTCAGTTTGTCAAATACCCCAA288690.36641241139068037No Hit
TGAGGTAGTAGTTTGTACAGTT256030.3249595402970518No Hit
CAACACCAGTCGATGGGCTGT253490.3217357101507623No Hit
TTCTCACTACTGCACTTGACTAG248220.315046897209445No Hit
AACATTCAACGCTGTCGGTGAGTT238120.30222773009230947No Hit
TGTAAACATCCTACACTCTCAGCT236240.2998415880942683No Hit
CTTCTCACTACTGCACTTGACTAG222430.28231359820440277No Hit
CCCCCACTGCTAAATTTGACTGGCTT221450.2810697582267005No Hit
AACGGAATCCCAAAAGCAGCTG212020.2691009715024838No Hit
CTCCCACTGCTTCACTTGACTAG196400.24927568532727018No Hit
CAAAGAATTCTCCTTTTGGGCT195670.2483491514663287No Hit
CACCCGGCTGTGTGCACATGTGC185400.23531421618979576No Hit
TAGCAGCACGTAAATATTGGC174030.2208831339995154No Hit
TAGCTTATCAGACTGATGTTGACA157950.2004740045694619No Hit
CAAAGAATTCTCCTTTTGGGCTT157130.19943324050648656No Hit
TGTCAGTTTGTCAAATACCCCA156920.1991667033684075No Hit
CCCCCCACTGCTAAATTTGACTGGCT153690.19506710833985821No Hit
TGAGGTAGTAGTTTGTGCTGTT152870.19402634427688284No Hit
CACTAGATTGTGAGCTCCTGGA150000.19038367005646906No Hit
CACTAGATTGTGAGCTCCTGGAA139780.1774121960032883No Hit
ACTGGACTTGGAGTCAGAAGGCT138000.17515297645195155No Hit
TATTGCACTTGTCCCGGCCTGTT135520.1720052997736846No Hit
ACTGGACTTGGAGTCAGAAGGCA135100.17147222549752647No Hit
TTCAAGTAATCCAGGATAGGCTT134820.1711168426467544No Hit
TATTGCACTTGTCCCGGCCTGTAG134080.1701776165411425No Hit
TGTAAACATCCCCGACTGGAAGC129010.16374264849323383No Hit
CCCCCCACTGCTAAATTTGACTGG128700.16334918890845046No Hit
CTCCCACTGCTTCACTTGACTAGCCT125760.15961766897534366No Hit
TGAGAACTGAATTCCATGGGTT122520.15550538170212394No Hit
ACCCCCCACTGCTAAATTTGACTG119700.15192616870506231No Hit
TTCAAGTAATCCAGGATAGGC117650.1493242585476239No Hit
CTACGCCTGTCTGAGCGTCGCT114020.14471697373225736No Hit
CAACGGAATCCCAAAAGCAGCT112010.14216583255350065No Hit
TATTGCACTTGTCCCGGCCTGTAAT111170.14109968400118444No Hit
CCCATAAAGTAGAAAGCACTA110780.14060468645903762No Hit
ACCACAGGGTAGAACCACGGAC107760.1367716285685674No Hit
TTATAAAGCAATGAGACTGATT106480.13514702125075218No Hit
TGATGTAGTAGGTTGTATAGTT106240.13484240737866182No Hit
AGCAGCATTGTACAGGGCTATGT105320.13367472086898213No Hit
TTCACAGTGGCTAAGTTCCGC104480.13260857231666592No Hit
TGAGGTAGTAGGTTGTGTGGTT103020.13075550459478294No Hit
ATCCCGGACGAGCCCCCA101780.12918166625564947No Hit
AACATTCAACGCTGTCGGTGA100840.12798859525662895No Hit
TTCACAGTGGCTAAGTTCCG100420.12745552098047083No Hit
ATCCCACCGCTGCCACCA99250.12597052835403036No Hit
ACTGGACTTGGAGTCAGAAGG99080.12575476019463303No Hit
CAAAGTGCTGTTCGTGCAGGTAG94480.11991632764623465No Hit
TGTAAACATCCTACACTCAGCT94340.1197386362208486No Hit
TACGCCTGTCTGAGCGTCGCT91670.11634980689384346No Hit
AACATTCAACGCTGTCGGTGAGA91310.11589288608570794No Hit
TCGTACGACTCTTAGCGG90010.11424289427855187No Hit
TAGCAGCACGTAAATATTGGCGT89070.11304982327953134No Hit
GGAATACCGGGTGCTGTAGGCTTT88760.11265636369474796No Hit
TGAGGTAGTAGGTTGTATAGT86800.11016868373934342No Hit
TATTGCACTTGTCCCGGCCTGTAAA86530.10982599313324179No Hit
ATCACATTGCCAGGGATTTCC85840.10895022825098202No Hit
TTCACAGTGGCTAAGTTCTG85640.10869638335757341No Hit
CAAAGTGCTGTTCGTGCAGGT84780.10760485031591632No Hit
AGCAGCATTGTACAGGGCTATG83850.1064244715615662No Hit
TTCACAGTGGCTAAGTTCTGC83380.10582793606205593No Hit
AGCTCGGTCTGAGGCCCCTCAGT82390.10457140383968325No Hit
TATTGCACTTGTCCCGGCCTGTTT82170.10429217445693377No Hit
AGAGGTAGTAGGTTGCATAGTT80540.10222333857565345No Hit
TAGCAGCACATCATGGTTTACA79200.10052257778981567No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)