FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6350073
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG69308710.91463043023285No Hit
CCCCCCACTGCTAAATTTGACTG3300925.198239453310221No Hit
AACATTCAACGCTGTCGGTGAGT2850294.488594068131185No Hit
AACATTCAACGCTGTCGGTGAG2243723.533376702913494No Hit
AAGCTGCCAGTTGAAGAACTGT2007303.161066022390609No Hit
TAGCAGCACGTAAATATTGGCG1893832.9823751632461546No Hit
TTCAAGTAATCCAGGATAGGCT1722232.712142049390613No Hit
CCCCCACTGCTAAATTTGACTG1427442.247911165745654No Hit
CGCCTGTCTGAGCGTCGCT1108641.745869693151559No Hit
TGAGGTAGTAGGTTGTATAGTT850081.338693271715144No Hit
TAGCTTATCAGACTGATGTTGAC807411.2714971938117876No Hit
TATTGCACTTGTCCCGGCCTGTAA769561.2118915798290824No Hit
TATTGCACTTGTCCCGGCCTGT768641.2104427775869664No Hit
TAGCTTATCAGACTGATGTTGACT700581.103262907371301No Hit
TAGCTTATCAGACTGATGTTGA698001.0991999619531934No Hit
TATTGCACTTGTCCCGGCCTGTAT626850.9871540059460733No Hit
ACTGGACTTGGAGTCAGAAGGC465710.7333931436693719No Hit
CATAAAGTAGAAAGCACTACT458210.7215822558260354No Hit
TATTGCACTTGTCCCGGCCTGTA454680.7160232646144382No Hit
AGCAGCATTGTACAGGGCTATGA426580.6717718048280705No Hit
TCGTACGACTCTTAGCGG407400.6415674276500444No Hit
TGTAAACATCCTTGACTGGAAGCT380420.5990797271149482No Hit
TGAGGTAGTAGATTGTATAGTT376900.5935364837538087No Hit
CAACGGAATCCCAAAAGCAGCTGT309680.48767943297659727No Hit
CAACACCAGTCGATGGGCTGT297160.467963124203454No Hit
TTCAAGTAATTCAGGATAGGTT288080.45366407598778785No Hit
CATTGCACTTGTCTCGGTCTGA286610.4513491419704939No Hit
CCCATAAAGTAGAAAGCACT274360.43205802515971076No Hit
TGTAAACATCCCCGACTGGAAGCT274260.43190054665513294No Hit
CAACGGAATCCCAAAAGCAGCT254500.40078279415055545No Hit
TACGCCTGTCTGAGCGTCGCT241800.38078302406917214No Hit
ACGCCTGTCTGAGCGTCGCT212930.3353189797975551No Hit
CTACGCCTGTCTGAGCGTCGCT204420.32191755905798247No Hit
CACCCGGCTGTGTGCACATGTGC198950.31330348485757564No Hit
TGAGGTAGTAGTTTGTACAGTT191380.3013823620610346No Hit
CCCATAAAGTAGAAAGCACTAC190290.29966584636113636No Hit
AACATTCAACGCTGTCGGTGAGTT187970.29601234505493085No Hit
AACGGAATCCCAAAAGCAGCTG185300.29180766898270305No Hit
ATCCCGGACGAGCCCCCA181230.2853982938463857No Hit
TTAATGCTAATCGTGATAGGGGTT173390.2730519790874845No Hit
TGTCAGTTTGTCAAATACCCCAA165930.26130408264597904No Hit
GCCTGTCTGAGCGTCGCT157720.2483750974201399No Hit
GCGTTCGCGCTTTCCCCTG156710.2467845645239039No Hit
TCGTTTCCCGGCCAATGCACCA145900.22976113817904142No Hit
TGAGGTAGTAGTTTGTGCTGTT131260.20670628510884836No Hit
TTCTCACTACTGCACTTGACTAG130290.2051787436144435No Hit
ACTGGACTTGGAGTCAGAAGGCT128930.2030370359521851No Hit
CTCCCACTGCTTCACTTGACTAG128090.2017142165137314No Hit
TGAGGTAGTAGGTTGTGTGGTT127960.20150949445778024No Hit
ACCGGGTGCTGTAGGCTT127780.20122603314954016No Hit
TAGCAGCACGTAAATATTGGC124960.1967851393204456No Hit
ACTGGACTTGGAGTCAGAAGGCA121510.19135213091251077No Hit
ACCACAGGGTAGAACCACGGAC118430.18650179297151387No Hit
ATCCCACCGCTGCCACCA114860.18087981035808565No Hit
TGAGAACTGAATTCCATGGGTT113820.1792420339104763No Hit
TATTGCACTTGTCCCGGCCTGTT113520.17876959839674283No Hit
ACTGGACTTGGAGTCAGAAGG112890.17777748381790257No Hit
CACTAGATTGTGAGCTCCTGGA112770.17758850961240918No Hit
TGTAAACATCCTACACTCTCAGCT102660.16166743279959142No Hit
AGCAGCATTGTACAGGGCTATG96490.15195100906713985No Hit
CTTCTCACTACTGCACTTGACTAG92670.14593533019226707No Hit
TATTGCACTTGTCCCGGCCTGTAG92490.14565186888402698No Hit
AGCAGCATTGTACAGGGCTATGT92320.14538415542624472No Hit
TTCACAGTGGCTAAGTTCCG89820.14144719281179918No Hit
TGTCAGTTTGTCAAATACCCCA88410.13922674589725187No Hit
TTATAAAGCAATGAGACTGATT88360.13914800664496296No Hit
TAGCTTATCAGACTGATGTTGACA87200.13732125599186026No Hit
TGATGTAGTAGGTTGTATAGTT84750.13346303262970363No Hit
TTCACAGTGGCTAAGTTCTG84690.13336854552695693No Hit
TTCAAGTAATCCAGGATAGGC84430.1329591014150546No Hit
CACTAGATTGTGAGCTCCTGGAA84150.1325181616022367No Hit
TTCAAGTAATCCAGGATAGGCTT81740.1287229296419112No Hit
CAAAGTGCTGTTCGTGCAGGTAG81400.1281875027263466No Hit
TAGTGGGTTATCAGAACT80700.12708515319430186No Hit
CCCCCACTGCTAAATTTGACTGGCTT79690.12549462029806585No Hit
AATGACACGATCACTCCCGTTG79560.1252898982421147No Hit
TTCACAGTGGCTAAGTTCCGC78290.12328992123397636No Hit
TGAGGTAGTAGGTTGTATAGT77600.12220331955238939No Hit
ACCGGGTGCTGTAGGCTTT76060.11977815058189095No Hit
AACATTCAACGCTGTCGGTGA75720.11924272366632635No Hit
CAAAGTGCTGTTCGTGCAGGT74370.11711676385452578No Hit
TGTAAACATCCCCGACTGGAAGC74040.11659708478941896No Hit
AACATTCAACGCTGTCGGTGAGA73290.11541599600508529No Hit
TTCACAGTGGCTAAGTTCTGC71770.11302232273550242No Hit
CAAAGAATTCTCCTTTTGGGCT71280.11225067806307108No Hit
CTCCCACTGCTTCACTTGACTAGCCT69760.10985700479348821No Hit
AATGCACCCGGGCAAGGATTCT69270.10908536012105687No Hit
CAACGGAATCCCAAAAGCAGCTGA67820.10680192180467847No Hit
CCCCCCACTGCTAAATTTGACTGG66570.10483344049745572No Hit
TCGTTCGCGCTTTCCCCTG66500.10472320554425123No Hit
AGAGGTAGTAGGTTGCATAGTT66240.1043137614323489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)