FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5414407
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4805958.876225965281147No Hit
CCCCCCACTGCTAAATTTGACTG2563144.733925617339073No Hit
AACATTCAACGCTGTCGGTGAGT2238284.133933780744595No Hit
AAGCTGCCAGTTGAAGAACTGT2233094.124348243491855No Hit
TTCAAGTAATCCAGGATAGGCT2079703.84104852110305No Hit
TAGCAGCACGTAAATATTGGCG1841583.4012589005591933No Hit
AACATTCAACGCTGTCGGTGAG1497422.7656214244699373No Hit
CCCCCACTGCTAAATTTGACTG1351632.4963583269599052No Hit
CATAAAGTAGAAAGCACTACT912231.684819778047716No Hit
TGAGGTAGTAGGTTGTATAGTT760281.4041796266885735No Hit
TATTGCACTTGTCCCGGCCTGT691481.2771112330491594No Hit
CGCCTGTCTGAGCGTCGCT637441.1773034424637823No Hit
TAGCTTATCAGACTGATGTTGAC587911.085825280589361No Hit
TATTGCACTTGTCCCGGCCTGTAA582291.075445565876374No Hit
TAGCTTATCAGACTGATGTTGA577731.0670235909491104No Hit
TATTGCACTTGTCCCGGCCTGTAT496190.9164253813944907No Hit
TAGCTTATCAGACTGATGTTGACT487800.9009296862980563No Hit
CCCATAAAGTAGAAAGCACT412990.7627612774584548No Hit
TGAGGTAGTAGATTGTATAGTT390910.7219811883369684No Hit
CCCATAAAGTAGAAAGCACTAC332420.6139545845002047No Hit
TATTGCACTTGTCCCGGCCTGTA319530.590147729936076No Hit
TCGTACGACTCTTAGCGG307060.5671165835889322No Hit
TGTAAACATCCTTGACTGGAAGCT289760.5351647927464633No Hit
ATCCCGGACGAGCCCCCA289300.5343152075564324No Hit
CAACGGAATCCCAAAAGCAGCTGT279740.5166586109983974No Hit
CAACACCAGTCGATGGGCTGT277460.5124476235347657No Hit
ACTGGACTTGGAGTCAGAAGGC272110.5025665783898403No Hit
TTCAAGTAATTCAGGATAGGTT268690.4962500971943926No Hit
AGCAGCATTGTACAGGGCTATGA259570.4794061473398657No Hit
CATTGCACTTGTCTCGGTCTGA239160.44171042184305687No Hit
GCCTGTCTGAGCGTCGCT221540.4091676152162185No Hit
TGAGGTAGTAGTTTGTACAGTT199640.36871997247343985No Hit
TGTAAACATCCCCGACTGGAAGCT198010.3657094858218084No Hit
TTAATGCTAATCGTGATAGGGGTT177850.32847549140653814No Hit
TCCACCACGTTCCCGTGG172890.31931474674881294No Hit
CAACGGAATCCCAAAAGCAGCT172680.31892689264032054No Hit
ATCCCACCGCTGCCACCA161720.29868460202566965No Hit
CTACGCCTGTCTGAGCGTCGCT156710.28943151115163673No Hit
ACCGGGTGCTGTAGGCTT152270.28123116714351176No Hit
TGAGAACTGAATTCCATGGGTT149430.2759859020572336No Hit
ACGCCTGTCTGAGCGTCGCT147110.2717010376205557No Hit
TGTCAGTTTGTCAAATACCCCAA145400.26854279702283185No Hit
CACCCGGCTGTGTGCACATGTGC142280.2627803931252305No Hit
AACGGAATCCCAAAAGCAGCTG134280.2480049985159963No Hit
AACATTCAACGCTGTCGGTGAGTT130870.2417069865638102No Hit
TGTAAACATCCTACACTCTCAGCT126790.23417153531310078No Hit
TACGCCTGTCTGAGCGTCGCT122340.22595272206171424No Hit
TCGTTTCCCGGCCAATGCACCA121790.22493691368232938No Hit
TTCAAGTAATCCAGGATAGGC119330.22039347983998986No Hit
TTCTCACTACTGCACTTGACTAG114720.21187915869641866No Hit
TTCACAGTGGCTAAGTTCCGC112350.207501948043433No Hit
TGAGGTAGTAGTTTGTGCTGTT107580.1986921190076771No Hit
TTCACAGTGGCTAAGTTCCG106790.19723304879001521No Hit
TAGCAGCACGTAAATATTGGC101870.18814618110533618No Hit
CTCCCACTGCTTCACTTGACTAG98490.18190357688293474No Hit
TGAGGTAGTAGGTTGTGTGGTT98290.18153419201770388No Hit
TATTGCACTTGTCCCGGCCTGTT93170.17207793946779398No Hit
TGTAAACATCCTACACTCAGCT90220.16662951270563886No Hit
TCCCCGGCACCTCCACCA89750.16576145827234637No Hit
ACCGGGTGCTGTAGGCTTT89650.1655767658397309No Hit
AACATTCAACGCTGTCGGTGA87460.16153200156545305No Hit
CCCATAAAGTAGAAAGCACTA86640.16001752361800653No Hit
ACCACAGGGTAGAACCACGGAC86610.15996211588822193No Hit
TTCACAGTGGCTAAGTTCTGC84870.15674846756071348No Hit
TGAGGTAGTAGGTTGTATAGT81250.150062601500035No Hit
TTATAAAGCAATGAGACTGATT81080.14974862436458877No Hit
CACCCGTAGAACCGACCTTGCG80650.14895444690434242No Hit
TGTCAGTTTGTCAAATACCCCA78960.1458331447931417No Hit
TGATGTAGTAGGTTGTATAGTT78620.14520519052224926No Hit
TCCTGTCTGAGCGTCGCT77970.14400468971024896No Hit
TTCAAGTAATCCAGGATAGGCTT77540.14321051225000264No Hit
ACCGGGTGCTGTAGGCTTA73730.13617373056735482No Hit
CTTCTCACTACTGCACTTGACTAG73440.1356381225127701No Hit
TTCACAGTGGCTAAGTTCTG73150.13510251445818536No Hit
ACTGGACTTGGAGTCAGAAGG73150.13510251445818536No Hit
ACTGGACTTGGAGTCAGAAGGCT72650.1341790522951082No Hit
CACTAGATTGTGAGCTCCTGGA71850.1327015128341848No Hit
CAAAGTGCTGTTCGTGCAGGT71130.1313717273193537No Hit
AATGACACGATCACTCCCGTTG70690.1305590806158458No Hit
TAGCAGCACATCATGGTTTACA69330.12804726353227602No Hit
AGAGGTAGTAGGTTGCATAGTT68920.12729002455855276No Hit
AACCGGGCGGAAACACCA68760.12699451666636807No Hit
CATAAAGTAGAAAGCACTAC65720.12137986671485908No Hit
CAAAGAATTCTCCTTTTGGGCT64650.119403657685874No Hit
ACTGGACTTGGAGTCAGAAGGCA63760.11775989503559668No Hit
TATTGCACTTGTCCCGGCCTGTAG63080.1165039864938118No Hit
TGAGAACTGAATTCCATAGGCTGT62660.11572827827682698No Hit
AGCAGCATTGTACAGGGCTATG62650.11570980903356544No Hit
CCCCCACTGCTAAATTTGACTGGCTT62260.11498950854636528No Hit
GCGTTCGCGCTTTCCCCTG62230.11493410081658065No Hit
TGTAAACATCCCCGACTGGAAGC60950.11257003767910319No Hit
CCCCCCACTGCTAAATTTGACTGG60370.1114988215699337No Hit
TCAGGCTCAGTCCCCTCCCGA59820.11048301319054885No Hit
TATTGCACTTGTCCCGGCCTGA58000.10712161091694805No Hit
AGCAGCATTGTACAGGGCTATGT57290.10581029464537851No Hit
AACATTCAACGCTGTCGGTGAGA55810.1030768466426702No Hit
TTCACCACCTTCTCCACCCAGC55690.10285521572353168No Hit
TAGCAGCACGTAAATATTGGCGT55400.10231960766894693No Hit
TAGCAGCACATAATGGTTTGT54430.10052809107257729No Hit
TCAGGCTCAGTCCCCTCCCGAT54190.10008482923430026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)