FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7115035
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG82919411.654109923563272No Hit
CCCCCCACTGCTAAATTTGACTG6113388.592199476179667No Hit
AACATTCAACGCTGTCGGTGAGT3023454.249381766920331No Hit
TAGCAGCACGTAAATATTGGCG2512503.531254589752545No Hit
AAGCTGCCAGTTGAAGAACTGT2310493.2473346933641225No Hit
TTCAAGTAATCCAGGATAGGCT2181513.066056597051174No Hit
AACATTCAACGCTGTCGGTGAG2147553.018326684267892No Hit
CCCCCACTGCTAAATTTGACTG2111712.9679544794930734No Hit
TATTGCACTTGTCCCGGCCTGTAA1462912.0560826475203564No Hit
TATTGCACTTGTCCCGGCCTGTAT1142261.6054172607724349No Hit
TAGCTTATCAGACTGATGTTGACT1110191.5603436947253246No Hit
TAGCTTATCAGACTGATGTTGAC1026381.4425508799324247No Hit
TGAGGTAGTAGGTTGTATAGTT867461.2191928781797983No Hit
TAGCTTATCAGACTGATGTTGA678900.9541766133265683No Hit
TGTAAACATCCTTGACTGGAAGCT677680.9524619344809968No Hit
TATTGCACTTGTCCCGGCCTGT618700.8695670506188656No Hit
TTAATGCTAATCGTGATAGGGGTT545830.7671501264575649No Hit
TATTGCACTTGTCCCGGCCTGTA512900.7208678523717733No Hit
AGCAGCATTGTACAGGGCTATGA507540.7133345092469678No Hit
CAACGGAATCCCAAAAGCAGCTGT494200.6945854799027693No Hit
TGTAAACATCCCCGACTGGAAGCT479830.6743888118610801No Hit
TGAGGTAGTAGATTGTATAGTT467330.6568203810662914No Hit
CATAAAGTAGAAAGCACTACT460880.6477550707761802No Hit
ACTGGACTTGGAGTCAGAAGGC441980.6211916034144597No Hit
TTCAAGTAATTCAGGATAGGTT372090.5229629931546367No Hit
CCCATAAAGTAGAAAGCACTAC336950.4735746205043264No Hit
CCCCCACTGCTAAATTTGACTGGCTT262700.36921814158328103No Hit
CCCATAAAGTAGAAAGCACT250320.3518183677241222No Hit
AACATTCAACGCTGTCGGTGAGTT231120.32483325802332663No Hit
TGAGAACTGAATTCCATGGGTT230520.3239899733451768No Hit
CAAAGAATTCTCCTTTTGGGCT228160.3206730536111207No Hit
TTCTCACTACTGCACTTGACTAG216650.3044960425352792No Hit
TGAGGTAGTAGTTTGTACAGTT214550.30154454616175463No Hit
CATTGCACTTGTCTCGGTCTGA211560.2973421775156412No Hit
TGTAAACATCCTACACTCTCAGCT204480.2873914183134728No Hit
TGTCAGTTTGTCAAATACCCCAA204190.2869838307190337No Hit
CTTCTCACTACTGCACTTGACTAG183890.2584526991082967No Hit
AACGGAATCCCAAAAGCAGCTG182640.25669585602881784No Hit
CGCCTGTCTGAGCGTCGCT180120.2531540603805884No Hit
TGAGAACTGAATTCCATAGGCTGT175320.24640778295538954No Hit
CAAAGAATTCTCCTTTTGGGCTT161920.22757442514337597No Hit
TAGCAGCACGTAAATATTGGC160960.2262251696583362No Hit
CCCCCCACTGCTAAATTTGACTGGCT158940.2233861112418983No Hit
TAGCTTATCAGACTGATGTTGACA156070.2193523995314148No Hit
CACCCGGCTGTGTGCACATGTGC155960.21919779734042066No Hit
TATTGCACTTGTCCCGGCCTGTAG154750.21749717323948514No Hit
CAACACCAGTCGATGGGCTGT152830.21479866226940558No Hit
ACTGGACTTGGAGTCAGAAGGCA148260.2083756439708308No Hit
CACTAGATTGTGAGCTCCTGGAA144450.20302078626457917No Hit
ACTGGACTTGGAGTCAGAAGGCT143520.20171369501344685No Hit
CACTAGATTGTGAGCTCCTGGA131440.18473556349336298No Hit
ACCCCCCACTGCTAAATTTGACTG129010.18132026054685607No Hit
CTCCCACTGCTTCACTTGACTAG128870.18112349412195441No Hit
TATTGCACTTGTCCCGGCCTGTAAT124430.17488318750364545No Hit
TGAGGTAGTAGTTTGTGCTGTT124090.1744053261860272No Hit
TATTGCACTTGTCCCGGCCTGTT123470.17353393201860567No Hit
CTCCCACTGCTTCACTTGACTAGCCT119740.1682915122694407No Hit
TTCAAGTAATCCAGGATAGGCTT119440.16786986993036576No Hit
CCCCCCACTGCTAAATTTGACTGG118830.16701253050758008No Hit
TGTAAACATCCCCGACTGGAAGC118580.1666611618916843No Hit
GGAATACCGGGTGCTGTAGGCTTT115920.16292259981855325No Hit
TATTGCACTTGTCCCGGCCTGTAAA114720.16123603046225352No Hit
TGTCAGTTTGTCAAATACCCCA114180.16047707425191865No Hit
CTACGCCTGTCTGAGCGTCGCT106260.14934571650034048No Hit
CAACGGAATCCCAAAAGCAGCT103620.1456352639164811No Hit
AGCAGCATTGTACAGGGCTATGT99280.13953550474453041No Hit
TGAGGTAGTAGGTTGTGTGGTT96240.1352628623752378No Hit
TTCAAGTAATCCAGGATAGGC94510.132831391553239No Hit
TGATGTAGTAGGTTGTATAGTT91180.1281511615895073No Hit
AGCTCGGTCTGAGGCCCCTCAGT87380.12281035862789151No Hit
AACATTCAACGCTGTCGGTGA86730.12189680022656249No Hit
ACTGGACTTGGAGTCAGAAGG85370.11998535495608947No Hit
AACATTCAACGCTGTCGGTGAGA85020.11949343889383539No Hit
TTCACAGTGGCTAAGTTCCG84570.11886097538522297No Hit
TATTGCACTTGTCCCGGCCTGTTT84360.11856582574787053No Hit
ATGCACCTGGGCAAGGATTCTGA80380.1129720373828098No Hit
CCCATAAAGTAGAAAGCACTA79970.11239579285274072No Hit
TTATAAAGCAATGAGACTGATT79510.11174927459949248No Hit
ACCACAGGGTAGAACCACGGAC78680.1105827307947185No Hit
CAAAGTGCTGTTCGTGCAGGTAG78320.11007675998782858No Hit
TACGCCTGTCTGAGCGTCGCT74920.10529814681164605No Hit
AGCAGCATTGTACAGGGCTATG73940.1039207818373346No Hit
GGAATACCGGGTGCTGTAGGCTT73740.10363968694461798No Hit
TAGCAGCACGTAAATATTGGCGT73710.10359752271071047No Hit
TTAATGCTAATCGTGATAGGGGT73440.10321804460554306No Hit
ATCACATTGCCAGGGATTTCC73290.10300722343600559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)