FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8766345
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG88544710.100526502208162No Hit
CCCCCCACTGCTAAATTTGACTG6739557.687981707313596No Hit
AACATTCAACGCTGTCGGTGAGT3560914.062023568545386No Hit
TAGCAGCACGTAAATATTGGCG2592732.9575952121437155No Hit
TTCAAGTAATCCAGGATAGGCT2562442.923042613540763No Hit
CCCCCACTGCTAAATTTGACTG2501452.8534697185657194No Hit
AACATTCAACGCTGTCGGTGAG2430282.772284230200842No Hit
AAGCTGCCAGTTGAAGAACTGT2375562.7098636889148215No Hit
TATTGCACTTGTCCCGGCCTGTAA1727791.9709354354636968No Hit
GCCTGTCTGAGCGTCGCT1598341.8232684203051557No Hit
TATTGCACTTGTCCCGGCCTGTAT1404971.6026861822116287No Hit
TAGCTTATCAGACTGATGTTGACT1127971.2867050064764733No Hit
TAGCTTATCAGACTGATGTTGAC1111701.2681453901255313No Hit
TGAGGTAGTAGGTTGTATAGTT1021221.1649324775604886No Hit
TAGCTTATCAGACTGATGTTGA884531.009006604234718No Hit
TATTGCACTTGTCCCGGCCTGT853230.9733018721029117No Hit
TATTGCACTTGTCCCGGCCTGTA674790.7697506771636298No Hit
TGTAAACATCCTTGACTGGAAGCT646310.7372627931024845No Hit
TGAGGTAGTAGATTGTATAGTT602390.6871620954913364No Hit
CATAAAGTAGAAAGCACTACT568310.6482861443395166No Hit
AGCAGCATTGTACAGGGCTATGA550890.6284146927824538No Hit
ACTGGACTTGGAGTCAGAAGGC525490.5994402456211796No Hit
GGAATACCGGGTGCTGTAGGCTTT522400.5959154014586467No Hit
TGTAAACATCCCCGACTGGAAGCT507100.5784622895859106No Hit
CAACGGAATCCCAAAAGCAGCTGT505170.5762606878921603No Hit
TCGTACGACTCTTAGCGG481010.548700741300964No Hit
TCCTGTCTGAGCGTCGCT446760.5096308666838917No Hit
TTCAAGTAATTCAGGATAGGTT434720.49589652243894117No Hit
CCCATAAAGTAGAAAGCACTAC393830.4492522254143545No Hit
CAACGGAATCCCAAAAGCAGCT393210.4485449751293156No Hit
GGAATACCGGGTGCTGTAGGCTT362730.4137756385357866No Hit
CCCCCACTGCTAAATTTGACTGGCTT359530.41012531448397255No Hit
CCCATAAAGTAGAAAGCACT320800.3659449861943604No Hit
CATTGCACTTGTCTCGGTCTGA297610.3394915440813703No Hit
TTCTCACTACTGCACTTGACTAG276940.3159127321591838No Hit
AACATTCAACGCTGTCGGTGAGTT271810.3100608064136194No Hit
TGAGGTAGTAGTTTGTACAGTT270530.30860067679289377No Hit
CTTCTCACTACTGCACTTGACTAG239770.273511936844831No Hit
CTCCCACTGCTTCACTTGACTAG238700.2722913597400056No Hit
CGCCTGTCTGAGCGTCGCT232450.2651618205763063No Hit
CCCCCCACTGCTAAATTTGACTGGCT218510.24926009642559127No Hit
CACCCGGCTGTGTGCACATGTGC215270.2455641433231295No Hit
AACGGAATCCCAAAAGCAGCTG208720.2380923862795726No Hit
TCGTTTCCCGGCCAATGCACCA202900.2314533594103358No Hit
CAACACCAGTCGATGGGCTGT201910.2303240404068058No Hit
CAAAGAATTCTCCTTTTGGGCT199440.22750644652931182No Hit
TTAATGCTAATCGTGATAGGGGTT193400.22061645988151277No Hit
TATTGCACTTGTCCCGGCCTGTAG192520.2196126207672639No Hit
TGTAAACATCCTACACTCTCAGCT188890.2154717844209873No Hit
CACTAGATTGTGAGCTCCTGGAA169590.1934557674834837No Hit
TATTGCACTTGTCCCGGCCTGTT167570.19115150042577608No Hit
CACTAGATTGTGAGCTCCTGGA165560.18885864063073035No Hit
TAGCTTATCAGACTGATGTTGACA165200.18844797917490128No Hit
ACTGGACTTGGAGTCAGAAGGCT155790.17771374501003553No Hit
TGAGGTAGTAGTTTGTGCTGTT155670.17757685785809252No Hit
ACTGGACTTGGAGTCAGAAGGCA155630.17753122880744485No Hit
TAGCAGCACGTAAATATTGGC153460.17505585280980843No Hit
TGTCAGTTTGTCAAATACCCCAA147000.16768676113020878No Hit
CTCCCACTGCTTCACTTGACTAGCCT146050.16660307117732648No Hit
ACCCCCCACTGCTAAATTTGACTG144190.16448132032220952No Hit
CCCCCCACTGCTAAATTTGACTGG140400.16015796777334224No Hit
TTCAAGTAATCCAGGATAGGC137300.15662171634814737No Hit
TGTAAACATCCCCGACTGGAAGC134350.1532565738628813No Hit
AACCGGGCGGAAACACCA133930.15277746883108068No Hit
TGAATACCGGGTGCTGTAGGCTTT133670.1524808800018708No Hit
CAAAGAATTCTCCTTTTGGGCTT132470.15111200848244052No Hit
TTCAAGTAATCCAGGATAGGCTT130080.1483856727062419No Hit
TATTGCACTTGTCCCGGCCTGTAAA129830.1481004911396939No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC125290.1429215938911827No Hit
CAACGGAATCCCAAAAGCAGCTGA124390.14189494025161No Hit
TTCACAGTGGCTAAGTTCCG122530.13977318939649305No Hit
ACTGGACTTGGAGTCAGAAGG122260.13946519330462126No Hit
TATTGCACTTGTCCCGGCCTGTAAT118760.13547265137294962No Hit
ATCCCGGACGAGCCCCCA116670.13308853347660854No Hit
TGAGAACTGAATTCCATGGGTT115550.13181092005847364No Hit
AGCAGCATTGTACAGGGCTATGT115120.1313204077640111No Hit
TATTGCACTTGTCCCGGCCTGTTT110580.1261415105154999No Hit
AACATTCAACGCTGTCGGTGA110530.1260844742021903No Hit
TGAGGTAGTAGGTTGTGTGGTT110070.12555974011974202No Hit
CTACGCCTGTCTGAGCGTCGCT108840.124156646812326No Hit
AGCTCGGTCTGAGGCCCCTCAGT108180.12340376747663935No Hit
CCCATAAAGTAGAAAGCACTA107460.12258244456498119No Hit
TTCACAGTGGCTAAGTTCTG102960.11744917636711766No Hit
TGTCAGTTTGTCAAATACCCCA102830.11730088195251272No Hit
AACATTCAACGCTGTCGGTGAGA102700.11715258753790776No Hit
ACCACAGGGTAGAACCACGGAC102450.11686740597135978No Hit
GTCAGGATGGCCGAGCGGTCTAAGG97520.11124362547903373No Hit
TTATAAAGCAATGAGACTGATT96820.11044511709269941No Hit
TCGTTTCCCGGGCGGCGCACCA95580.10903061652262146No Hit
TGAGGTAGTAGGTTGTATAGT95320.10873402769341156No Hit
TGAATACCGGGTGCTGTAGGCTT94110.10735374891131938No Hit
CAAAGTGCTGTTCGTGCAGGTAG92180.10515214721756902No Hit
TATTGCACTTGTCCCGGCCTGA90920.10371483212216721No Hit
TTCACAGTGGCTAAGTTCTGC88960.1014790086404311No Hit
AACACCCTGATTGCTCCTATCTGATT88150.10055502036481567No Hit
AGCAGCATTGTACAGGGCTATG88110.100509391314168No Hit
ATGCACCTGGGCAAGGATTCTGA88030.10041813321287264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)