FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947565

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947565
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8051430
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG199667524.799010858940584No Hit
CCCCCACTGCTAAATTTGACTG5800087.203788643756451No Hit
CAACGGAATCCCAAAAGCAGCTG5331386.6216560288048205No Hit
TAGCAGCACGTAAATATTGGCG2541893.1570665086823086No Hit
TTCAAGTAATCCAGGATAGGCT1977342.455886718259986No Hit
AAGCTGCCAGTTGAAGAACTGT1961982.4368093618152304No Hit
AACATTCAACGCTGTCGGTGAGT1811582.2500102466269967No Hit
AACATTCAACGCTGTCGGTGAG1249531.551935494688521No Hit
TAGCTTATCAGACTGATGTTGACT1233151.5315912825423559No Hit
TAGCTTATCAGACTGATGTTGAC1195601.4849536045149743No Hit
TATTGCACTTGTCCCGGCCTGTAT1186701.4738996675124791No Hit
TATTGCACTTGTCCCGGCCTGTAA1114771.3845615002552343No Hit
TGTAAACATCCTTGACTGGAAGCT815731.0131492169713952No Hit
TAGCTTATCAGACTGATGTTGA648320.8052234199390668No Hit
TATTGCACTTGTCCCGGCCTGT609520.7570332226697618No Hit
TGAGGTAGTAGGTTGTATAGTT604630.7509597673953571No Hit
TATTGCACTTGTCCCGGCCTGTA502260.6238146515587915No Hit
TGAGGTAGTAGATTGTATAGTT483380.6003654009287791No Hit
AGCAGCATTGTACAGGGCTATGA467170.5802323313995154No Hit
CATAAAGTAGAAAGCACTACT427740.531259664432281No Hit
TGTAAACATCCCCGACTGGAAGCT417910.5190506531137947No Hit
ACTGGACTTGGAGTCAGAAGGC357460.443970822574375No Hit
TTCAAGTAATTCAGGATAGGTT357100.443523697032701No Hit
CAACGGAATCCCAAAAGCAGCTGT298280.3704683515847495No Hit
TTCTCACTACTGCACTTGACTAG291940.3625939739897136No Hit
CCCATAAAGTAGAAAGCACTAC279470.34710604203228496No Hit
CTTCTCACTACTGCACTTGACTAG249760.3102057646902476No Hit
CATTGCACTTGTCTCGGTCTGA243850.30286545371443335No Hit
ACCCCCCACTGCTAAATTTGACTG235260.29219654148393515No Hit
CCCCCCACTGCTAAATTTGACTGG207950.25827710108639085No Hit
CAAAGAATTCTCCTTTTGGGCT202760.2518310411939245No Hit
TGTAAACATCCTACACTCTCAGCT201470.2502288413362595No Hit
TGAGGTAGTAGTTTGTACAGTT184910.2296610664192572No Hit
CCCCCCACTGCTAAATTTGACTGA183930.2284438913335892No Hit
TCGTACGACTCTTAGCGG183170.2274999596344997No Hit
GACCCGCGGGCGCTCTCCAGTCCT172710.21450847861808398No Hit
CTCCCACTGCTTCACTTGACTAG172470.2142103949236347No Hit
GCCTGTCTGAGCGTCGCT169160.2100993239710213No Hit
CCCCCCACTGCTAAATTTGACTGT163300.20282111376488401No Hit
TATTGCACTTGTCCCGGCCTGTAG162810.20221252622205002No Hit
CACTAGATTGTGAGCTCCTGGA160180.19894602573704298No Hit
CACTAGATTGTGAGCTCCTGGAA159630.19826291727059664No Hit
TGTCAGTTTGTCAAATACCCCAA150500.18692331672758752No Hit
TAGCTTATCAGACTGATGTTGACA150100.186426510570172No Hit
CCCATAAAGTAGAAAGCACT147060.1826507837738141No Hit
CAAAGAATTCTCCTTTTGGGCTT144440.17939670344274247No Hit
TAGCAGCACGTAAATATTGGC143510.1782416291267514No Hit
AGCTCGGTCTGAGGCCCCTCAGT141380.1755961363385138No Hit
CACCCGGCTGTGTGCACATGTGC141220.17539741387554758No Hit
TGAGGTAGTAGTTTGTGCTGTT137280.17050387322500477No Hit
AACATTCAACGCTGTCGGTGAGTT134520.1670759107388377No Hit
TATTGCACTTGTCCCGGCCTGTT133640.16598293719252358No Hit
CCCCCACTGCTAAATTTGACTGGCTT130240.1617600848544917No Hit
TTCAAGTAATCCAGGATAGGCTT124310.1543949335708067No Hit
CCCCCCACTGCTAAATTTGACTGGCT118970.14776257136930954No Hit
AACGGAATCCCAAAAGCAGCTG118320.14695526136350934No Hit
CAACACCAGTCGATGGGCTGT112640.13990061392820904No Hit
TGTCAGTTTGTCAAATACCCCA112380.13957768992588895No Hit
TATTGCACTTGTCCCGGCCTGTTT111370.13832325437841475No Hit
TGTAAACATCCCCGACTGGAAGC106720.13254788279845942No Hit
TATTGCACTTGTCCCGGCCTGTAAT99200.12320792703904772No Hit
CCCCCCACTGCTAAATTTGACTGTA98420.12223915503208747No Hit
ACTGGACTTGGAGTCAGAAGGCA94720.11764369807599395No Hit
CTCCCACTGCTTCACTTGACTAGCCT93510.11614085944981203No Hit
TCAGTGCACTACAGAACTTTGT93150.11569373390813806No Hit
ACTGGACTTGGAGTCAGAAGGCT92840.11530870913614102No Hit
CCCCCACAACCGCGCTTGACTAG91460.11359472789305751No Hit
AGCAGCATTGTACAGGGCTATGT88690.11015434525295507No Hit
CGCCTGTCTGAGCGTCGCT87070.10814228031542222No Hit
TTCACAGTGGCTAAGTTCCGC82360.10229238781185454No Hit
TTCAAGTAATCCAGGATAGGC81350.10103795226438037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)