FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9819641
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG195944719.95436493044909No Hit
CAACGGAATCCCAAAAGCAGCTG7138607.269715868431442No Hit
CCCCCACTGCTAAATTTGACTG6824966.950315189730459No Hit
AACATTCAACGCTGTCGGTGAGT3169203.2274092301337696No Hit
AAGCTGCCAGTTGAAGAACTGT2807182.8587399478249766No Hit
TAGCAGCACGTAAATATTGGCG2639082.6875524268147886No Hit
TTCAAGTAATCCAGGATAGGCT2392772.4367184095630376No Hit
AACATTCAACGCTGTCGGTGAG2090212.1286012390880686No Hit
GCCTGTCTGAGCGTCGCT1610211.639784998249936No Hit
TATTGCACTTGTCCCGGCCTGTAT1302491.3264130531859566No Hit
TATTGCACTTGTCCCGGCCTGTAA1272581.2959536911787304No Hit
TATTGCACTTGTCCCGGCCTGT1123831.14447157487733No Hit
TGAGGTAGTAGGTTGTATAGTT964620.982337337994332No Hit
TAGCTTATCAGACTGATGTTGACT952950.9704529931389548No Hit
TAGCTTATCAGACTGATGTTGAC911900.9286490208756104No Hit
TATTGCACTTGTCCCGGCCTGTA715860.7290083211799698No Hit
TGTAAACATCCTTGACTGGAAGCT689380.7020419585603995No Hit
CATAAAGTAGAAAGCACTACT658100.6701874335324479No Hit
TAGCTTATCAGACTGATGTTGA636990.6486897026072542No Hit
TGAGGTAGTAGATTGTATAGTT591540.6024049148028935No Hit
ACTGGACTTGGAGTCAGAAGGC546740.5567820656580011No Hit
AGCAGCATTGTACAGGGCTATGA518350.5278706217467624No Hit
TGTAAACATCCCCGACTGGAAGCT463910.47243071309837087No Hit
CCCATAAAGTAGAAAGCACTAC401280.4086503773406787No Hit
ATCCCGGACGAGCCCCCA372610.37945379062228446No Hit
CAACGGAATCCCAAAAGCAGCTGT365820.3725390775487617No Hit
TTCTCACTACTGCACTTGACTAG348280.3546769174148016No Hit
TTCAAGTAATTCAGGATAGGTT343290.34959526524442186No Hit
TCGTACGACTCTTAGCGG341740.3480167961333821No Hit
CATTGCACTTGTCTCGGTCTGA341580.34785385738643604No Hit
CCCATAAAGTAGAAAGCACT312200.31793422997846865No Hit
CGCCTGTCTGAGCGTCGCT309250.31493004683165093No Hit
TTAATGCTAATCGTGATAGGGGTT266740.2716392585024238No Hit
TGAGGTAGTAGTTTGTACAGTT254660.25933738310799753No Hit
CTCCCACTGCTTCACTTGACTAG243990.24847140542103321No Hit
CACTAGATTGTGAGCTCCTGGA243620.2480946095687205No Hit
CACCCGGCTGTGTGCACATGTGC238520.24290093700981533No Hit
AACCGGGCGGAAACACCA237600.24196403921487555No Hit
AGCTCGGTCTGAGGCCCCTCAGT231520.2357723668309259No Hit
TCCTGTCTGAGCGTCGCT228470.23266634696726693No Hit
TGAGAACTGAATTCCATGGGTT225600.22974363319392227No Hit
GACCCGCGGGCGCTCTCCAGTCCT222110.2261895317761617No Hit
CTTCTCACTACTGCACTTGACTAG218570.22258451199998044No Hit
CAACACCAGTCGATGGGCTGT216640.22061906336494377No Hit
AACATTCAACGCTGTCGGTGAGTT210950.2148245541766751No Hit
CCCCCCACTGCTAAATTTGACTGG206810.2106085140994462No Hit
CCCCCCACTGCTAAATTTGACTGA200380.20406041320655205No Hit
AACGGAATCCCAAAAGCAGCTG200340.20401967851981553No Hit
ACCCCCCACTGCTAAATTTGACTG193240.19678927162408485No Hit
TATTGCACTTGTCCCGGCCTGTT193100.19664670022050704No Hit
TGAGGTAGTAGTTTGTGCTGTT191190.19470161892883864No Hit
TGTAAACATCCTACACTCTCAGCT191100.19460996588368148No Hit
TATTGCACTTGTCCCGGCCTGTAG180900.18422262076587118No Hit
TGTCAGTTTGTCAAATACCCCAA180740.1840596820189251No Hit
CACTAGATTGTGAGCTCCTGGAA178260.18153413144126146No Hit
CCCCCCACTGCTAAATTTGACTGT156780.159659604663755No Hit
ACTGGACTTGGAGTCAGAAGGCT135310.1377952615579327No Hit
TTCAAGTAATCCAGGATAGGCTT135040.13752030242246127No Hit
TAGCAGCACGTAAATATTGGC134390.13685836376299296No Hit
TGTAAACATCCCCGACTGGAAGC132400.13483181309785153No Hit
ACTGGACTTGGAGTCAGAAGG127390.12972979358410353No Hit
CCCCCACTGCTAAATTTGACTGGCTT127110.12944465077694794No Hit
ACCACAGGGTAGAACCACGGAC126920.12925116101494952No Hit
CAAAGAATTCTCCTTTTGGGCT126580.12890491617768918No Hit
ACCGGGTGCTGTAGGCTTA125480.12778471229243513No Hit
ACCGGGTGCTGTAGGCTT124620.12690891652760014No Hit
GGAATACCGGGTGCTGTAGGCTTT123500.12576834529897785No Hit
ACTGGACTTGGAGTCAGAAGGCA120750.1229678355858427No Hit
TTCAAGTAATCCAGGATAGGC117220.1193729994813456No Hit
CCCCCACAACCGCGCTTGACTAG116780.11892491792724398No Hit
TGAGGTAGTAGGTTGTGTGGTT115740.11786581607209469No Hit
AACCATTCGTAGACGACCTGCTTC113640.11572724501842788No Hit
TGTCAGTTTGTCAAATACCCCA113280.11536063283779927No Hit
CCCCCCACTGCTAAATTTGACTGTA112410.11447465340128016No Hit
GGAATACCGGGTGCTGTAGGCTT112010.11406730653391503No Hit
TCGTTTCCCGGGCGGCGCACCA110910.112947102648661No Hit
TATTGCACTTGTCCCGGCCTGTTT110850.11288600061855622No Hit
TGAGAACTGAATTCCATAGGCTGT105550.1074886546259685No Hit
AGCAGCATTGTACAGGGCTATGT105180.10711185877365578No Hit
CAAAGAATTCTCCTTTTGGGCTT104770.10669432823460655No Hit
CAACGGAATCCCAAAAGCAGCT104430.10634808339734621No Hit
CCCCCCACTGCTAAATTTGACTGGCT103650.10555375700598423No Hit
TTATAAAGCAATGAGACTGATT103070.10496310404830482No Hit
CTACGCCTGTCTGAGCGTCGCT101510.1033744512655809No Hit
TACGCCTGTCTGAGCGTCGCT100560.10240700245558874No Hit
TTCACAGTGGCTAAGTTCCG100460.10230516573874748No Hit
AGCAGCATTGTACAGGGCTATG99970.10180616582622522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)