FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13495928
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG194112214.38301982642468No Hit
CAACGGAATCCCAAAAGCAGCTG11976898.874447166582394No Hit
CCCCCACTGCTAAATTTGACTG6957885.155540248880996No Hit
AAGCTGCCAGTTGAAGAACTGT4328593.2073303888402487No Hit
TTCAAGTAATCCAGGATAGGCT3927892.9104260188702846No Hit
TAGCAGCACGTAAATATTGGCG3850392.853001290463316No Hit
AACATTCAACGCTGTCGGTGAGT2774042.0554644334202137No Hit
TAGCTTATCAGACTGATGTTGAC2106011.5604780938368967No Hit
AACATTCAACGCTGTCGGTGAG2062401.5281646434391174No Hit
TAGCTTATCAGACTGATGTTGACT1634781.2113135161954036No Hit
TATTGCACTTGTCCCGGCCTGTAA1565011.159616441344382No Hit
TATTGCACTTGTCCCGGCCTGTAT1513901.121745759165283No Hit
TCGTACGACTCTTAGCGG1389261.0293919766021276No Hit
TATTGCACTTGTCCCGGCCTGT1282030.9499383814140087No Hit
TGTAAACATCCTTGACTGGAAGCT1272420.9428177150915447No Hit
TGAGGTAGTAGGTTGTATAGTT1260620.9340743370889353No Hit
TAGCTTATCAGACTGATGTTGA1155200.8559618871707081No Hit
CATAAAGTAGAAAGCACTACT1123030.8321250676500349No Hit
TTCAAGTAATTCAGGATAGGTT923180.6840433647838073No Hit
TGAGGTAGTAGATTGTATAGTT919200.6810943271185205No Hit
AGCAGCATTGTACAGGGCTATGA876830.6496996723752527No Hit
GGAATACCGGGTGCTGTAGGCTTT806610.5976691636173519No Hit
TATTGCACTTGTCCCGGCCTGTA793080.5876439174838514No Hit
CAACGGAATCCCAAAAGCAGCTGT722500.5353466616004472No Hit
TGTAAACATCCCCGACTGGAAGCT698060.5172374956357206No Hit
ACTGGACTTGGAGTCAGAAGGC596300.44183697482677736No Hit
GGAATACCGGGTGCTGTAGGCTT590360.43743564725597234No Hit
CAACGGAATCCCAAAAGCAGCT551670.40876774090673873No Hit
CCCATAAAGTAGAAAGCACTAC547790.4058927996652027No Hit
CATTGCACTTGTCTCGGTCTGA527720.39102164741839174No Hit
TTAATGCTAATCGTGATAGGGGTT519890.3852198974386941No Hit
TTCTCACTACTGCACTTGACTAG438270.3247423963731875No Hit
TGTAAACATCCTACACTCTCAGCT431510.31973347812762487No Hit
CCCATAAAGTAGAAAGCACT416710.3087672074124877No Hit
TGAGGTAGTAGTTTGTACAGTT377950.2800474335666284No Hit
TGAGAACTGAATTCCATGGGTT376970.2793212886138693No Hit
CAAAGAATTCTCCTTTTGGGCT351510.2604563391268833No Hit
TGAATACCGGGTGCTGTAGGCTTT344580.25532145696094405No Hit
CACCCGGCTGTGTGCACATGTGC328660.2435253062997965No Hit
ACCCCCCACTGCTAAATTTGACTG323700.23985012368175052No Hit
CTTCTCACTACTGCACTTGACTAG307060.2275204787695963No Hit
TCGTTTCCCGGCCAATGCACCA300960.22300059692078972No Hit
AACGGAATCCCAAAAGCAGCTG293860.21773975083447394No Hit
TGAGGTAGTAGTTTGTGCTGTT293600.21754710013272152No Hit
TAGCAGCACGTAAATATTGGC292350.2166208948358349No Hit
TGAGAACTGAATTCCATAGGCTGT284630.21090065092226337No Hit
TGTCAGTTTGTCAAATACCCCAA276830.20512112986969108No Hit
TGTCAGTTTGTCAAATACCCCA264770.19618510116532925No Hit
TAGCTTATCAGACTGATGTTGACA259080.19196901465390154No Hit
CACTAGATTGTGAGCTCCTGGA259070.19196160501152645No Hit
TGAATACCGGGTGCTGTAGGCTT250580.18567081863507276No Hit
CTCCCACTGCTTCACTTGACTAG238590.1767866574273366No Hit
CAAAGAATTCTCCTTTTGGGCTT238280.17655695851370873No Hit
TATTGCACTTGTCCCGGCCTGTT229730.17022171428300448No Hit
CCCCCCACTGCTAAATTTGACTGG223830.1658500252816998No Hit
TTCAAGTAATCCAGGATAGGCTT222480.16484972356106226No Hit
CACTAGATTGTGAGCTCCTGGAA221530.16414580753542846No Hit
CAACACCAGTCGATGGGCTGT216350.16030761278513045No Hit
CCCCCACTGCTAAATTTGACTGGCTT211750.15689917729258782No Hit
AACATTCAACGCTGTCGGTGAGTT203150.1505268848500081No Hit
AGCTCGGTCTGAGGCCCCTCAGT200590.14863001640198437No Hit
TGTAAACATCCCCGACTGGAAGC199290.1476667628932223No Hit
TCAGTGCACTACAGAACTTTGT192270.14246519394590726No Hit
TATTGCACTTGTCCCGGCCTGTAG188940.13999778303500138No Hit
GACCCGCGGGCGCTCTCCAGTCCT188610.13975326483662331No Hit
TTCAAGTAATCCAGGATAGGC181710.13464061159780935No Hit
AGCAGCATTGTACAGGGCTATGT177020.13116548932389088No Hit
TTCACAGTGGCTAAGTTCCGC176620.13086910362888718No Hit
CCCCCCACTGCTAAATTTGACTGT173480.12854247592310805No Hit
CCCCCCACTGCTAAATTTGACTGA172580.12787560810934973No Hit
TCGTTTCCCGGGCGGCGCACCA169890.12588241431044978No Hit
TTATAAAGCAATGAGACTGATT167630.12420783513367883No Hit
CCCCCCACTGCTAAATTTGACTGTA164720.12205162920252687No Hit
ATCACATTGCCAGGGATTTCC156090.11565710783282188No Hit
CTACGCCTGTCTGAGCGTCGCT149620.1108630692161369No Hit
TACGCCTGTCTGAGCGTCGCT147290.1091366225427403No Hit
CCCATAAAGTAGAAAGCACTA147210.10907734540373955No Hit
GGAATACCGGGTGCTGTAGGCTTA144260.10689150090308722No Hit
TATTGCACTTGTCCCGGCCTGTTT139810.10359421004617096No Hit
CAAAGTGCTGTTCGTGCAGGTAG139750.1035497521919204No Hit
TTCACAGTGGCTAAGTTCTGC139390.10328300506641708No Hit
TGAGGTAGTAGGTTGTGTGGTT139250.10317927007316577No Hit
GGGAATACCGGGTGCTGTAGGCTTT136820.10137872697601824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)