FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11304583
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG245056621.67763286801468No Hit
CCCCCACTGCTAAATTTGACTG9521818.422964385329383No Hit
CAACGGAATCCCAAAAGCAGCTG9450848.360184537545525No Hit
AAGCTGCCAGTTGAAGAACTGT3209142.8387955575185746No Hit
TAGCAGCACGTAAATATTGGCG3130012.7687973983648932No Hit
AACATTCAACGCTGTCGGTGAGT2646542.341121295672737No Hit
TTCAAGTAATCCAGGATAGGCT2433962.1530736693250874No Hit
AACATTCAACGCTGTCGGTGAG2154931.9062445735503908No Hit
TAGCTTATCAGACTGATGTTGAC1168471.0336250350853278No Hit
TAGCTTATCAGACTGATGTTGACT949550.8399690638743595No Hit
TGAGGTAGTAGGTTGTATAGTT857780.7587895988733064No Hit
CATAAAGTAGAAAGCACTACT857360.7584180681410363No Hit
TATTGCACTTGTCCCGGCCTGT841470.7443618221034779No Hit
TATTGCACTTGTCCCGGCCTGTAA830550.734702023064451No Hit
TAGCTTATCAGACTGATGTTGA752620.6657653802886847No Hit
TATTGCACTTGTCCCGGCCTGTAT720560.6374052010587211No Hit
TGTAAACATCCTTGACTGGAAGCT646670.5720423300886022No Hit
TATTGCACTTGTCCCGGCCTGTA542640.4800177060931836No Hit
CGCCTGTCTGAGCGTCGCT538910.47671815935183104No Hit
AGCAGCATTGTACAGGGCTATGA508590.44989717886984415No Hit
TGAGGTAGTAGATTGTATAGTT421460.3728222438633959No Hit
ACTGGACTTGGAGTCAGAAGGC410030.3627113003637551No Hit
CAACGGAATCCCAAAAGCAGCTGT398430.35244997537724304No Hit
TTCAAGTAATTCAGGATAGGTT385150.34070252746164986No Hit
CAACACCAGTCGATGGGCTGT364760.32266559500691006No Hit
CATTGCACTTGTCTCGGTCTGA363530.3215775407195471No Hit
TTAATGCTAATCGTGATAGGGGTT361410.31970219511856385No Hit
TGTAAACATCCCCGACTGGAAGCT352670.31197081749941596No Hit
CCCATAAAGTAGAAAGCACT339320.30016144779511106No Hit
CAACGGAATCCCAAAAGCAGCT329840.2917754684095822No Hit
CCCCCCACTGCTAAATTTTACTG323820.2864501945803751No Hit
CCCATAAAGTAGAAAGCACTAC306410.2710493611307909No Hit
CCACCCACTGCTAAATTTGACTG276920.2449625961435287No Hit
CCCACCACTGCTAAATTTGACTG274420.24275110368953903No Hit
TGTCAGTTTGTCAAATACCCCAA263140.23277284973713758No Hit
CACCCCACTGCTAAATTTGACTG252610.22345804352093307No Hit
TCGTACGACTCTTAGCGG224610.19868932803624867No Hit
AACGGAATCCCAAAAGCAGCTG222000.19638052991428345No Hit
CACCCGGCTGTGTGCACATGTGC220990.1954870869628716No Hit
TTCTCACTACTGCACTTGACTAG220750.1952747836872886No Hit
CCCCACACTGCTAAATTTGACTG213190.1885872305064238No Hit
TGAGGTAGTAGTTTGTACAGTT210060.18581844195402875No Hit
CCCCCAACTGCTAAATTTGACTG201580.17831705955009575No Hit
ACCCCCACTGCTAAATTTGACTG200670.1775120762968435No Hit
AACATTCAACGCTGTCGGTGAGTT194930.1724344896224832No Hit
TAGCAGCACGTAAATATTGGC189440.16757805219352187No Hit
GCCTGTCTGAGCGTCGCT186220.16472964991278316No Hit
CCCCCCACTGCTAAATTTGACTGG185830.16438465708996078No Hit
ACCCCCCACTGCTAAATTTGACTG182990.1618724016622285No Hit
TGTAAACATCCTACACTCTCAGCT178800.1581659403093418No Hit
TGAGGTAGTAGTTTGTGCTGTT177860.15733441914664167No Hit
ACCGGGTGCTGTAGGCTT173560.15353065212577943No Hit
TTCACAGTGGCTAAGTTCCG171670.15185876383056324No Hit
GCGTTCGCGCTTTCCCCTG165650.1465334900013561No Hit
CACTAGATTGTGAGCTCCTGGA162350.1436143199620897No Hit
CTACGCCTGTCTGAGCGTCGCT161610.14295971819570877No Hit
CAAAGAATTCTCCTTTTGGGCT160530.14200435345558524No Hit
CTTCTCACTACTGCACTTGACTAG159550.14113744841362127No Hit
TGTCAGTTTGTCAAATACCCCA154230.13643139247153124No Hit
TACGCCTGTCTGAGCGTCGCT152300.1347241202970512No Hit
GCCCCCACTGCTAAATTTGACTG150770.1333706869152095No Hit
TCGTTTCCCGGCCAATGCACCA147920.1308495855176613No Hit
TGAGAACTGAATTCCATGGGTT146230.12935461661876427No Hit
TATTGCACTTGTCCCGGCCTGTT139030.122985518351274No Hit
GACCCGCGGGCGCTCTCCAGTCCT137670.12178246645630361No Hit
ACCACAGGGTAGAACCACGGAC134300.11880137462832552No Hit
ACGCCTGTCTGAGCGTCGCT130240.11520991088304629No Hit
CGCCCCACTGCTAAATTTGACTG126690.11206959159838092No Hit
TTCACAGTGGCTAAGTTCTG125650.11114961073752122No Hit
ATCCCGGACGAGCCCCCA124370.1100173266010785No Hit
CCCCCACTGCTAAATTTTACTG124050.10973425556696784No Hit
TGAGAACTGAATTCCATAGGCTGT121350.10734584371665898No Hit
TAGCTTATCAGACTGATGTTGACA120930.10697431298438871No Hit
ACTGGACTTGGAGTCAGAAGGCA117340.10379860982045953No Hit
CACTAGATTGTGAGCTCCTGGAA117140.10362169042414038No Hit
AGCAGCATTGTACAGGGCTATG115530.10219748928377101No Hit
CCCCCCACTGCTAAATTTGACTGA115110.10182595855150074No Hit
ACTGGACTTGGAGTCAGAAGG114630.10140135200033473No Hit
CCCCCCACTGCTAAATTTGACTGT114120.10095020753972085No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)