FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10370055
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG126902612.237408576907258No Hit
CAACGGAATCCCAAAAGCAGCTG7854307.574019617060855No Hit
CCCCCACTGCTAAATTTGACTG5491385.295420323228758No Hit
TAGCAGCACGTAAATATTGGCG3297273.179607051264434No Hit
TTCAAGTAATCCAGGATAGGCT3261953.145547444058879No Hit
AAGCTGCCAGTTGAAGAACTGT2417552.3312798244560904No Hit
AACATTCAACGCTGTCGGTGAGT2015191.9432780250442256No Hit
TAGCTTATCAGACTGATGTTGAC1807421.7429222892260454No Hit
TAGCTTATCAGACTGATGTTGACT1574091.5179186609907085No Hit
AACATTCAACGCTGTCGGTGAG1523761.4693846850378325No Hit
TAGCTTATCAGACTGATGTTGA1485461.432451419013689No Hit
CATAAAGTAGAAAGCACTACT1443521.392008046244692No Hit
TATTGCACTTGTCCCGGCCTGTAA1098661.0594543616210328No Hit
TATTGCACTTGTCCCGGCCTGTAT995210.9596959707542535No Hit
TGTAAACATCCTTGACTGGAAGCT955120.9210365808088771No Hit
TGAGGTAGTAGGTTGTATAGTT903650.8714032857106351No Hit
TTCAAGTAATTCAGGATAGGTT888930.8572085683248546No Hit
TATTGCACTTGTCCCGGCCTGT753920.7270163948021491No Hit
TGAGGTAGTAGATTGTATAGTT719250.6935835923724609No Hit
TATTGCACTTGTCCCGGCCTGTA562470.542398280433421No Hit
AGCAGCATTGTACAGGGCTATGA528880.5100069382467113No Hit
TCGTACGACTCTTAGCGG524330.5056193048156447No Hit
TTCTCACTACTGCACTTGACTAG463190.44666108328258625No Hit
TGTAAACATCCCCGACTGGAAGCT433910.4184259389173924No Hit
CAACGGAATCCCAAAAGCAGCTGT402260.38790536790788477No Hit
CATTGCACTTGTCTCGGTCTGA398310.384096323500695No Hit
CAACGGAATCCCAAAAGCAGCT355300.3426211336391176No Hit
CCCATAAAGTAGAAAGCACTAC354120.3414832418921597No Hit
ACTGGACTTGGAGTCAGAAGGC312780.30161845814703975No Hit
CAAAGAATTCTCCTTTTGGGCT310750.2996608986162561No Hit
CTTCTCACTACTGCACTTGACTAG299580.2888894996217474No Hit
CCCCCACTGCTAAATTTGACTGGCTT289270.2789474115614623No Hit
TGAGGTAGTAGTTTGTACAGTT288100.27781916296490233No Hit
CCCATAAAGTAGAAAGCACT286250.2760351801412818No Hit
GGAATACCGGGTGCTGTAGGCTTT282340.2722647083356839No Hit
AGAATTACTCTGAGACCT278600.26865817008685106No Hit
TGATGTAGTAGGTTGTATAGTT255250.24614141390764077No Hit
GCCTGTCTGAGCGTCGCT233290.22496505563374541No Hit
TAGCTTATCAGACTGATGTTGACA223340.21537012098778646No Hit
TAGCAGCACGTAAATATTGGC222360.2144250922487875No Hit
TGTCAGTTTGTCAAATACCCCAA222270.21433830389520594No Hit
TGTAAACATCCTACACTCTCAGCT221390.2134897066601865No Hit
TTATAAAGCAATGAGACTGATT221250.2133547025546152No Hit
CAACACCAGTCGATGGGCTGT219670.2118310847917393No Hit
GGAATACCGGGTGCTGTAGGCTT213390.20577518634182748No Hit
TGTCAGTTTGTCAAATACCCCA209130.20166720427230136No Hit
ACCCCCCACTGCTAAATTTGACTG206960.1995746406359465No Hit
TGATGTAGTAGATTGTATAGTT202820.19558237637119572No Hit
CACCCGGCTGTGTGCACATGTGC196270.18926611286053932No Hit
CAAAGAATTCTCCTTTTGGGCTT193520.18661424650110342No Hit
TGAATACCGGGTGCTGTAGGCTTT187800.18109836447347674No Hit
CTCCCACTGCTTCACTTGACTAG177080.17076090724687576No Hit
TTCACAGTGGCTAAGTTCCG168240.1622363622950891No Hit
TTCAAGTAATCCAGGATAGGCTT167820.16183134997837523No Hit
TGAGGTAGTAGTTTGTGCTGTT167160.16119490205211062No Hit
TCAGTGCACTACAGAACTTTGT163290.15746300284810447No Hit
TCGTTTCCCGGCCAATGCACCA162190.15640225630433013No Hit
TCCTGTCTGAGCGTCGCT161570.1558043809796573No Hit
TTCAAGTAATCCAGGATAGGC159610.15391432350165934No Hit
TTCACAGTGGCTAAGTTCCGC155600.15004742019208192No Hit
AACATTCAACGCTGTCGGTGAGTT149650.14430974570530242No Hit
CACTAGATTGTGAGCTCCTGGA149490.14415545529893525No Hit
TATTGCACTTGTCCCGGCCTGTT144190.13904458558802243No Hit
AACGGAATCCCAAAAGCAGCTG143830.1386974321736963No Hit
TGTAAACATCCCCGACTGGAAGC140920.1358912754078932No Hit
TTCACAGTGGCTAAGTTCTGC139530.13455087750257833No Hit
TGAATACCGGGTGCTGTAGGCTT138330.1333936994548245No Hit
CACTAGATTGTGAGCTCCTGGAA136430.13156150087921423No Hit
TTCACAGTGGCTAAGTTCTG130900.1262288387091486No Hit
AACCGGGCGGAAACACCA125890.1213976203597763No Hit
CCCCCCACTGCTAAATTTGACTGG125160.12069367038072604No Hit
TATTGCACTTGTCCCGGCCTGTAG124870.12041401901918554No Hit
AGCAGCATTGTACAGGGCTATGT122760.11837931428521835No Hit
CCCCCCACTGCTAAATTTGACTGGCT119980.1156985184745886No Hit
CCCATAAAGTAGAAAGCACTA117720.1135191664846522No Hit
TTTAAACATCCTTGACTGGAAGCT115460.1113398144947158No Hit
CGCCTGTCTGAGCGTCGCT113740.10968119262626862No Hit
CCCCCCACTGCTAAATTTGACTGA112640.10862044608249426No Hit
TGTCAGTTTGTCAAATACCCC107210.10338421541640812No Hit
TAGCAGCACGTAAATATTGGCGT105360.10160023259278761No Hit
AGCTCTAGAATTACTCTGAGACCT104950.10120486342647171No Hit
CAGTGCAATAGTATTGTCAAAGC103860.1001537600330953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)