FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10210722
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG7319307.168249218811364No Hit
CAACGGAATCCCAAAAGCAGCTG6374356.242800460143759No Hit
AACATTCAACGCTGTCGGTGAGT4950234.8480704890408335No Hit
AAGCTGCCAGTTGAAGAACTGT3484853.412932014014288No Hit
TTCAAGTAATCCAGGATAGGCT3142263.0774121555752867No Hit
TAGCAGCACGTAAATATTGGCG3021842.9594773023886067No Hit
CCCCCACTGCTAAATTTGACTG2291922.2446208994819368No Hit
AACATTCAACGCTGTCGGTGAG2254962.20842365505593No Hit
TATTGCACTTGTCCCGGCCTGTAA1566131.5338092644183243No Hit
TGAGGTAGTAGGTTGTATAGTT1552671.5206270428281174No Hit
TATTGCACTTGTCCCGGCCTGTAT1349351.321503023978128No Hit
TAGCTTATCAGACTGATGTTGACT1155061.131222650073129No Hit
GGAATACCGGGTGCTGTAGGCTTT1120931.0977970020141572No Hit
TATTGCACTTGTCCCGGCCTGT1084481.0620992325518215No Hit
TAGCTTATCAGACTGATGTTGAC1063921.0419635359771817No Hit
CGCCTGTCTGAGCGTCGCT907880.8891437843474731No Hit
TGAGGTAGTAGATTGTATAGTT853800.8361798509449184No Hit
TAGCTTATCAGACTGATGTTGA808430.7917461664317175No Hit
GGAATACCGGGTGCTGTAGGCTT801340.7848024850740232No Hit
CATAAAGTAGAAAGCACTACT699480.6850446031142557No Hit
TGTAAACATCCTTGACTGGAAGCT670800.6569564816278418No Hit
ACTGGACTTGGAGTCAGAAGGC627030.614089777392823No Hit
AGCAGCATTGTACAGGGCTATGA556130.544652963815879No Hit
TATTGCACTTGTCCCGGCCTGTA530790.5198359136601702No Hit
TGTAAACATCCCCGACTGGAAGCT528900.5179849182065676No Hit
TCGTACGACTCTTAGCGG468760.45908604700039823No Hit
TTCTCACTACTGCACTTGACTAG450970.44166318503236107No Hit
CTCCCACTGCTTCACTTGACTAG439670.4305963868177No Hit
CAACGGAATCCCAAAAGCAGCTGT437730.4286964232304043No Hit
TTCAAGTAATTCAGGATAGGTT422250.4135358890389925No Hit
CTTCTCACTACTGCACTTGACTAG402050.3937527630269436No Hit
ACCGGGTGCTGTAGGCTT359940.3525118008305387No Hit
CACCCGGCTGTGTGCACATGTGC356440.3490840314720154No Hit
TGAGGTAGTAGTTTGTACAGTT337950.3309756156322736No Hit
AGCTCGGTCTGAGGCCCCTCAGT336640.329692650529512No Hit
CCCATAAAGTAGAAAGCACTAC334620.3277143379283071No Hit
ACCGGGTGCTGTAGGCTTA334510.3276066080341821No Hit
AACCGGGCGGAAACACCA331950.32509943958909077No Hit
AACATTCAACGCTGTCGGTGAGTT323050.31638311179170286No Hit
CCCCCCACTGCTAAATTTGACTGGCT321690.3150511785552481No Hit
TGAATACCGGGTGCTGTAGGCTTT306600.3002725958066432No Hit
ACCGGGTGCTGTAGGCTTT297570.2914289508616531No Hit
CCCCCACTGCTAAATTTGACTGGCTT293490.28743315115228874No Hit
CATTGCACTTGTCTCGGTCTGA284740.2788637277559804No Hit
CCCATAAAGTAGAAAGCACT279210.27344785216951356No Hit
CCCCCCACTGCTAAATTTGACTGG262520.25710228914272665No Hit
TGAGGTAGTAGTTTGTGCTGTT237690.2327847139506883No Hit
TACGCCTGTCTGAGCGTCGCT236840.23195225567790406No Hit
GCCTGTCTGAGCGTCGCT231950.22716317220271004No Hit
TGTAAACATCCTACACTCTCAGCT229930.22518485960150514No Hit
CTACGCCTGTCTGAGCGTCGCT222810.21821179736359486No Hit
CGCGACCTCAGATCAGACGTGGCG218560.21404950599967368No Hit
TGAATACCGGGTGCTGTAGGCTT214910.21047483224007077No Hit
CAACACCAGTCGATGGGCTGT209910.20557801887075175No Hit
CGCGACCTCAGATCAGACGTGG207110.20283580338393306No Hit
CTCCCACTGCTTCACTTGACTAGCCT187430.18356194596229336No Hit
TGAGGTAGTAGGTTGTGTGGTT184650.18083931772895198No Hit
TATTGCACTTGTCCCGGCCTGTAG183640.17985016142834953No Hit
ATCCCGGACGAGCCCCCA178340.17465953925687136No Hit
ACGCCTGTCTGAGCGTCGCT177740.17407192165255309No Hit
GGAATACCGGGTGCTGTAGGCTTA177310.17365079570279163No Hit
AACGGAATCCCAAAAGCAGCTG174180.17058539053359792No Hit
TTCAAGTAATCCAGGATAGGCTT170900.16737308096332462No Hit
TATTGCACTTGTCCCGGCCTGTT166820.1633772812539603No Hit
CACTAGATTGTGAGCTCCTGGA162380.159028910982005No Hit
AACATTCAACGCTGTCGGTGA158040.15477847697743607No Hit
GCCCCCACTGCTAAATTTGACTG150630.14752139956410526No Hit
ACCCCCCACTGCTAAATTTGACTG147860.1448085649575025No Hit
TTCAAGTAATCCAGGATAGGC141110.1381978669089218No Hit
TGAGGTAGTAGGTTGTATAGT139830.13694428268637615No Hit
CAAAGAATTCTCCTTTTGGGCT138530.1356711112103532No Hit
AACATTCAACGCTGTCGGTGAGA138340.13548503230231906No Hit
ACTGGACTTGGAGTCAGAAGG134270.13149902621969337No Hit
CAAAGAATTCTCCTTTTGGGCTT134030.13126397917796606No Hit
GCGTTCGCGCTTTCCCCTG132980.13023564837040907No Hit
ACTGGACTTGGAGTCAGAAGGCT131950.12922690481632934No Hit
CGCGACCTCAGATCAGACGTGGC131600.128884127880477No Hit
AGAGGTAGTAGGTTGCATAGTT128930.12626922954126063No Hit
CCCCCACTGCTAAATTTGACTGGCT127100.12447699584808988No Hit
TGTAAACATCCCCGACTGGAAGC125740.1231450626116351No Hit
CAAAGTGCTGTTCGTGCAGGT124720.12214611268429401No Hit
TAGCAGCACGTAAATATTGGC124440.12187189113561216No Hit
ACCACAGGGTAGAACCACGGAC123390.12084356032805516No Hit
ACTGGACTTGGAGTCAGAAGGCA121130.11863020068512296No Hit
CACTAGATTGTGAGCTCCTGGAA120510.11802299582732739No Hit
CCCCCCACTGCTAAATTTGACTGT118250.11580963618439519No Hit
TTATAAAGCAATGAGACTGATT114500.11213702615740591No Hit
TAGCTTATCAGACTGATGTTGACA113280.11094220369529206No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC111370.10907162098821219No Hit
GTCAAGTAATCCAGGATAGGCT104990.1028232871289611No Hit
TATTGCACTTGTCCCGGCCTGTAAT104260.10210835237704052No Hit
GGAATACCGGGTGCTGTAGGCT104110.10196144797596095No Hit
AATGACACGATCACTCCCGTTG103510.10137383037164267No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)