FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6130619
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG4263536.9544853464226035No Hit
TAGCTTATCAGACTGATGTTGAC2409633.9304840180086216No Hit
CCCCCCACTGCTAAATTTGACTG2333343.8060430765637205No Hit
TTCAAGTAATCCAGGATAGGCT1477772.4104743746104593No Hit
AACATTCAACGCTGTCGGTGAGT1329382.168427038118011No Hit
AAGCTGCCAGTTGAAGAACTGT1296572.114908788166415No Hit
TAGCAGCACGTAAATATTGGCG1172201.912041834601041No Hit
AACATTCAACGCTGTCGGTGAG1033351.685555732626673No Hit
TAGCTTATCAGACTGATGTTGACT856841.3976402709090225No Hit
CCCCCACTGCTAAATTTGACTG844381.3773160589493492No Hit
TAGCTTATCAGACTGATGTTGA772631.2602805687321297No Hit
TATTGCACTTGTCCCGGCCTGT684631.1167387828211148No Hit
TCGTACGACTCTTAGCGG628191.0246763010390958No Hit
CAACGGAATCCCAAAAGCAGCT500860.8169811237658057No Hit
TGTAAACATCCTTGACTGGAAGCT472980.7715044761385433No Hit
TGAGGTAGTAGGTTGTATAGTT437030.7128643942805776No Hit
AGCAGCATTGTACAGGGCTATGA412980.6736350766537604No Hit
TATTGCACTTGTCCCGGCCTGTAA402020.6557575996812067No Hit
CATAAAGTAGAAAGCACTACT384930.6278811323946244No Hit
ACTGGACTTGGAGTCAGAAGGC373030.6084703681634759No Hit
TGAGGTAGTAGATTGTATAGTT357380.5829427664645284No Hit
TATTGCACTTGTCCCGGCCTGTAT343550.5603838698832858No Hit
TCGTTTCCCGGCCAATGCACCA341960.5577903307969391No Hit
TGTAAACATCCCCGACTGGAAGCT338280.5517876742952057No Hit
TAGCTTATCAGACTGATGTTGACA316620.5164568210811992No Hit
CCCCCACTGCTAAATTTGACTGGCTT313160.5108130190442434No Hit
TATTGCACTTGTCCCGGCCTGTA308010.5024125622551329No Hit
TTCAAGTAATTCAGGATAGGTT297110.4846329546820639No Hit
CCCATAAAGTAGAAAGCACTAC260030.4241496658004681No Hit
TTAATGCTAATCGTGATAGGGGTT249170.4064353044937224No Hit
CAACGGAATCCCAAAAGCAGCTGT239590.39080882370931874No Hit
CATTGCACTTGTCTCGGTCTGA232070.37854252564055935No Hit
CGCCTGTCTGAGCGTCGCT224600.3663577854047038No Hit
ACCGGGTGCTGTAGGCTT209600.3418904355335081No Hit
CTACGCCTGTCTGAGCGTCGCT204130.3329680086138121No Hit
TTCACAGTGGCTAAGTTCCGC192590.3141444607795722No Hit
TGAGGTAGTAGTTTGTGCTGTT191020.31158354482638706No Hit
CCAAGTCCTTCTGATCGAGGCCC185710.30292210297198374No Hit
CGCGACCTCAGATCAGAC167230.27277832793067064No Hit
CTCCCACTGCTTCACTTGACTAGCCT164490.26830895868753224No Hit
CACCCGGCTGTGTGCACATGTGC141780.2312653909825419No Hit
AACATTCAACGCTGTCGGTGAGTT139150.22697544897179225No Hit
CCCCCCACTGCTAAATTTGACTGGCT134850.21996147534204946No Hit
TGAGGTAGTAGTTTGTACAGTT129250.21082699805680308No Hit
ACCGGGTGCTGTAGGCTTT128740.20999510816118241No Hit
TGTAAACATCCTACACTCTCAGCT127040.20722214184244692No Hit
TACGCCTGTCTGAGCGTCGCT124860.2036662203278331No Hit
CCCATAAAGTAGAAAGCACT124220.2026222800666621No Hit
CAAAGAATTCTCCTTTTGGGCT123760.20187194800394542No Hit
TAGCAGCACGTAAATATTGGC118420.19316157144979976No Hit
GTCAGGATGGCCGAGCGGTCTAAGG116480.18999712753312512No Hit
TTCTCACTACTGCACTTGACTAG106700.17404441541710552No Hit
AACGGAATCCCAAAAGCAGCTG104290.1701133278711334No Hit
CCCCCCACTGCTAAATTTGACTGG102040.16644322539045403No Hit
TATTGCACTTGTCCCGGCCTGTT101020.16477944559921276No Hit
CAACACCAGTCGATGGGCTGT100280.16357238967223375No Hit
ATCACATTGCCAGGGATTTCC98510.16068524238743265No Hit
CTCCCACTGCTTCACTTGACTAG98260.16027745322291273No Hit
TGAGAACTGAATTCCATGGGTT95440.15567759144712792No Hit
AATGCACCCGGGCAAGGATTCT94620.15434004298750256No Hit
CAAAGAATTCTCCTTTTGGGCTT90810.14812533612021886No Hit
TTCACAGTGGCTAAGTTCTGC87390.14254678034958623No Hit
CAAGTCCTTCTGATCGAGGCCC86450.1410134930909913No Hit
CACTAGATTGTGAGCTCCTGGA84850.13840364243806375No Hit
TGTCAGTTTGTCAAATACCCCAA83150.13563067611932825No Hit
CTTCTCACTACTGCACTTGACTAG82970.13533706792087388No Hit
ACGCCTGTCTGAGCGTCGCT82660.1348314093568692No Hit
CAAAGTGCTGTTCGTGCAGGTAG82630.1347824746571268No Hit
ACTGGACTTGGAGTCAGAAGGCA78400.1278826819934496No Hit
TTATAAAGCAATGAGACTGATT77780.12687136486544018No Hit
ACTGGACTTGGAGTCAGAAGGCT77190.12590898243717313No Hit
ACCCCCCACTGCTAAATTTGACTG76960.12553381640581482No Hit
CAACGGAATCCCAAAAGCAGCTGA75380.12295658888604886No Hit
TGAGGTAGTAGGTTGTGTGGTT75170.12261404598785212No Hit
TTCAAGTAATCCAGGATAGGCTT73290.11954747147066226No Hit
TCGTTTCCCGGCCAACGCACCA73240.11946591363775827No Hit
TGTAAACATCCCCGACTGGAAGC73080.11920492857246552No Hit
CCTCGACACAAGGGTTTGT72940.11897656664033437No Hit
TGTCAGTTTGTCAAATACCCCA72590.11840566181000646No Hit
ACCACAGGGTAGAACCACGGAC71870.11723122901618906No Hit
GCGTTCGCGCTTTCCCCTG70720.11535539885939738No Hit
AGCAGCATTGTACAGGGCTATG67710.11044561731857745No Hit
ACCGGGTGCTGTAGGCTTA66640.10870027969443216No Hit
CACTAGATTGTGAGCTCCTGGAA66180.1079499476317155No Hit
ACTGGACTTGGAGTCAGAAGG65820.10736273123480679No Hit
AGCAGCATTGTACAGGGCTATGT65720.10719961556899882No Hit
TTCACCACCTTCTCCACCCAGC65210.10636772567337817No Hit
TTCAAGTAATCCAGGATAGGC64980.10599255964201984No Hit
TAGCAGCACATCATGGTTTACA62640.10217565306211329No Hit
TGTAAACATCCTACACTCAGCT61680.10060974267035677No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)