FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8785363
Sequences flagged as poor quality0
Sequence length18-26
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG6855757.803604700226957No Hit
CCCCCCACTGCTAAATTTGACTG5867136.678301169797993No Hit
TTCAAGTAATCCAGGATAGGCT2892643.2925674215169023No Hit
AACATTCAACGCTGTCGGTGAGT2354502.6800258566436015No Hit
TAGCAGCACGTAAATATTGGCG2209342.514796485927787No Hit
AAGCTGCCAGTTGAAGAACTGT2181632.483255387398335No Hit
CCCCCACTGCTAAATTTGACTG2008322.285984085119761No Hit
AACATTCAACGCTGTCGGTGAG1751751.9939415138566272No Hit
TATTGCACTTGTCCCGGCCTGTAA1185791.3497336421955473No Hit
TATTGCACTTGTCCCGGCCTGTAT1093051.2441716978569923No Hit
TGAGGTAGTAGGTTGTATAGTT1063871.210957361693535No Hit
GGAATACCGGGTGCTGTAGGCTTT1060491.207110053392216No Hit
TAGCTTATCAGACTGATGTTGAC955521.0876272272414924No Hit
TATTGCACTTGTCCCGGCCTGT934971.064236048072231No Hit
GCCTGTCTGAGCGTCGCT919331.046433710251927No Hit
TAGCTTATCAGACTGATGTTGACT846190.9631816010334462No Hit
GGAATACCGGGTGCTGTAGGCTT813840.9263589905163852No Hit
TAGCTTATCAGACTGATGTTGA742660.8453378648099117No Hit
CATAAAGTAGAAAGCACTACT678450.7722503896537912No Hit
TATTGCACTTGTCCCGGCCTGTA604200.6877348152831021No Hit
TGAGGTAGTAGATTGTATAGTT595720.6780823968229884No Hit
TTCAAGTAATTCAGGATAGGTT592940.6749180426579983No Hit
TGTAAACATCCTTGACTGGAAGCT546390.6219321842478222No Hit
TGAATACCGGGTGCTGTAGGCTTT469030.5338766309371622No Hit
AGCAGCATTGTACAGGGCTATGA447060.5088691269785892No Hit
ACCGGGTGCTGTAGGCTT426680.4856714514812877No Hit
TTAATGCTAATCGTGATAGGGGTT396710.45155789237166405No Hit
CAACGGAATCCCAAAAGCAGCTGT375880.42784800127211586No Hit
ACCGGGTGCTGTAGGCTTA369620.42072251311641873No Hit
TCCTGTCTGAGCGTCGCT366700.4173988029862853No Hit
TGAATACCGGGTGCTGTAGGCTT356200.40544710560053127No Hit
CATTGCACTTGTCTCGGTCTGA330350.37602316489369875No Hit
ACCGGGTGCTGTAGGCTTT329170.3746800217589188No Hit
TTCTCACTACTGCACTTGACTAG318510.36254620327014375No Hit
CTCCCACTGCTTCACTTGACTAG314230.35767446376433165No Hit
TGTAAACATCCCCGACTGGAAGCT313520.3568663013696759No Hit
CACCCGGCTGTGTGCACATGTGC308770.35145958112373954No Hit
ACTGGACTTGGAGTCAGAAGGC301900.3436397562627748No Hit
CCCCCACTGCTAAATTTGACTGGCTT287750.3275334212143539No Hit
CAACGGAATCCCAAAAGCAGCT281660.3206014367306166No Hit
CCCATAAAGTAGAAAGCACTAC276720.31497844767484284No Hit
CGCCTGTCTGAGCGTCGCT259310.2951613951523688No Hit
TCGTACGACTCTTAGCGG252020.2868635023959738No Hit
TGAGGTAGTAGTTTGTACAGTT249180.28363085281735084No Hit
AACCGGGCGGAAACACCA245630.27959004084407213No Hit
CTACGCCTGTCTGAGCGTCGCT227610.2590786516163305No Hit
CTTCTCACTACTGCACTTGACTAG209620.2386014100954053No Hit
TGATGTAGTAGGTTGTATAGTT208510.23733794494319702No Hit
CCCATAAAGTAGAAAGCACT206860.2354598210682928No Hit
TACGCCTGTCTGAGCGTCGCT182870.20815303818407957No Hit
CAACACCAGTCGATGGGCTGT180610.20558057760390774No Hit
GGAATACCGGGTGCTGTAGGCTTA169800.19327602058105056No Hit
AACATTCAACGCTGTCGGTGAGTT168910.19226297194549616No Hit
TATTGCACTTGTCCCGGCCTGTT168790.19212638111823038No Hit
TGAGGTAGTAGTTTGTGCTGTT167820.1910222719311655No Hit
TGTAAACATCCTACACTCTCAGCT167600.1907718554145116No Hit
CTCCCACTGCTTCACTTGACTAGCCT164800.1875847361116439No Hit
CCCCCCACTGCTAAATTTGACTGGCT155310.17678267818871002No Hit
AGCTCGGTCTGAGGCCCCTCAGT154490.17584930753572733No Hit
TGAGAACTGAATTCCATGGGTT148190.1686782891042749No Hit
TTCACAGTGGCTAAGTTCCG146310.166538366143778No Hit
CCCCCCACTGCTAAATTTGACTGG146010.1661968890756136No Hit
ATCCCGGACGAGCCCCCA145770.1659237074210821No Hit
TATTGCACTTGTCCCGGCCTGTAG144770.16478545052720076No Hit
AACGGAATCCCAAAAGCAGCTG144740.1647513028203843No Hit
ACCCCCCACTGCTAAATTTGACTG134090.1526288669005481No Hit
TAGCAGCACGTAAATATTGGC126700.14421714845476505No Hit
TTCAAGTAATCCAGGATAGGC126550.14404640992068285No Hit
GGAATACCGGGTGCTGTAGGCT122620.1395730603277292No Hit
CAAAGAATTCTCCTTTTGGGCT119460.13597616854306419No Hit
TGAGGTAGTAGGTTGTGTGGTT116330.1324134244652156No Hit
TTCACAGTGGCTAAGTTCTGC116000.13203779969023477No Hit
TGATGTAGTAGATTGTATAGTT115050.1309564556410475No Hit
TAGCTTATCAGACTGATGTTGACA113110.12874823726691773No Hit
TTCACAGTGGCTAAGTTCCGC113060.12869132442222364No Hit
CACTAGATTGTGAGCTCCTGGA111580.1270067042192793No Hit
TTCAAGTAATCCAGGATAGGCTT111570.12699532165034044No Hit
CGCGACCTCAGATCAGACGTGG105640.12024545826962414No Hit
TCCCACTGCTTCACTTGACTAG98180.11175406184126939No Hit
TTATAAAGCAATGAGACTGATT96610.10996699851787568No Hit
TGAGGTAGTAGGTTGTATAGT95890.10914745355428113No Hit
CAACGGAATCCCAAAAGCAGCTGA95670.10889703703762725No Hit
TATTGCACTTGTCCCGGCCTGTTT93690.1066432883877422No Hit
TTCACAGTGGCTAAGTTCTG93390.1063018113195778No Hit
TCGTTTCCCGGCCAATGCACCA92980.10583512599308645No Hit
TATTGCACTTGTCCCGGCCTGA90220.10269353696597397No Hit
CACTAGATTGTGAGCTCCTGGAA88810.10108859474560129No Hit
AGCAGCATTGTACAGGGCTATGT88660.1009178562115191No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)