FastQCFastQC Report
Sun 4 Sep 2022
EGAF00003947574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5081262
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG55299510.883024728896089No Hit
CAACGGAATCCCAAAAGCAGCTG3621897.127933966010806No Hit
AACATTCAACGCTGTCGGTGAGT1731273.407165385292079No Hit
CCCCCACTGCTAAATTTGACTG1639853.2272494510222063No Hit
TAGCAGCACGTAAATATTGGCG1397602.7504978094024675No Hit
TTCAAGTAATCCAGGATAGGCT1368472.6931695315061495No Hit
AACATTCAACGCTGTCGGTGAG1149592.2624104011956083No Hit
AAGCTGCCAGTTGAAGAACTGT1072432.110558361288987No Hit
TATTGCACTTGTCCCGGCCTGTAA843811.6606307645620322No Hit
TAGCTTATCAGACTGATGTTGAC781051.537118141123209No Hit
TAGCTTATCAGACTGATGTTGACT735031.4465500893282024No Hit
TATTGCACTTGTCCCGGCCTGTAT674771.3279575034705944No Hit
TGAGGTAGTAGGTTGTATAGTT405390.7978136140195093No Hit
CCCCCACTGCTAAATTTGACTGGCTT404970.7969870477058651No Hit
TAGCTTATCAGACTGATGTTGA394120.7756340846033919No Hit
TGTAAACATCCTTGACTGGAAGCT377560.7430437556654232No Hit
TATTGCACTTGTCCCGGCCTGT323070.6358066165452598No Hit
TATTGCACTTGTCCCGGCCTGTA309530.6091596930053991No Hit
CAACGGAATCCCAAAAGCAGCT295540.5816271627009196No Hit
AGCAGCATTGTACAGGGCTATGA263710.5189852442168894No Hit
TGTAAACATCCCCGACTGGAAGCT261510.5146556111454201No Hit
CATAAAGTAGAAAGCACTACT246410.48493858415488117No Hit
TGAGGTAGTAGATTGTATAGTT244640.481455197547381No Hit
ACTGGACTTGGAGTCAGAAGGC237060.4665376436011369No Hit
TCGTTTCCCGGCCAATGCACCA211040.4153298924558505No Hit
TTCTCACTACTGCACTTGACTAG210090.4134602781749888No Hit
CTTCTCACTACTGCACTTGACTAG208380.4100949724694377No Hit
CAACGGAATCCCAAAAGCAGCTGT202250.3980310403202984No Hit
TTCAAGTAATTCAGGATAGGTT200610.39480349566702133No Hit
CCCCCCACTGCTAAATTTGACTGGCT174580.3435760643714101No Hit
CGCCTGTCTGAGCGTCGCT171370.33725873611713No Hit
CCCCCCACTGCTAAATTTGACTGG170110.33477903717619756No Hit
CTCCCACTGCTTCACTTGACTAGCCT169120.3328307022940364No Hit
CTCCCACTGCTTCACTTGACTAG163450.32167205705984064No Hit
GTCAGGATGGCCGAGCGGTCTAAGG161520.3178737880471426No Hit
CCCATAAAGTAGAAAGCACTAC159240.3133867137730745No Hit
CACCCGGCTGTGTGCACATGTGC154170.30340887755837037No Hit
ACCCCCCACTGCTAAATTTGACTG153820.3027200722970002No Hit
CAAAGAATTCTCCTTTTGGGCT149440.29410016645471143No Hit
AACATTCAACGCTGTCGGTGAGTT137140.2698935815551333No Hit
CTACGCCTGTCTGAGCGTCGCT133140.26202152142518925No Hit
TAGCTTATCAGACTGATGTTGACA123140.24234137110032902No Hit
GCCTGTCTGAGCGTCGCT117120.23049392060476315No Hit
CATTGCACTTGTCTCGGTCTGA109880.21624549176956434No Hit
TGTAAACATCCTACACTCTCAGCT107460.21148289539094814No Hit
TAGCAGCACATCATGGTTTACA98880.19459732641221805No Hit
TATTGCACTTGTCCCGGCCTGTAG96910.1907203367982206No Hit
TGTCAGTTTGTCAAATACCCCAA96480.18987409033425162No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC92230.181510026446186No Hit
TGAGGTAGTAGTTTGTACAGTT91750.1805653792305927No Hit
CAAAGAATTCTCCTTTTGGGCTT89330.17580278285197654No Hit
TAGCAGCACGTAAATATTGGC86890.17100082617271065No Hit
TACGCCTGTCTGAGCGTCGCT82320.16200699747424951No Hit
CACTAGATTGTGAGCTCCTGGAA78960.15539446696509646No Hit
CCCATAAAGTAGAAAGCACT78180.15385941523975735No Hit
GGAATACCGGGTGCTGTAGGCTTT77250.15202916125954538No Hit
CAACGGAATCCCAAAAGCAGCTGA73050.14376349812310404No Hit
CACTAGATTGTGAGCTCCTGGA72860.14338957526693172No Hit
AACGGAATCCCAAAAGCAGCTG72490.1426614097049119No Hit
CTACGGGGATGATTTTACGAACTGAA70460.13866633918896526No Hit
ATCACATTGCCAGGGATTTCCA70180.13811529497986919No Hit
TTCACAGTGGCTAAGTTCTGC70020.13780041257467143No Hit
TATTGCACTTGTCCCGGCCTGTT69650.13707224701265158No Hit
TATTGCACTTGTCCCGGCCTGTAAA69240.1362653608493323No Hit
TGTAAACATCCCCGACTGGAAGC69090.1359701585944594No Hit
ATCACATTGCCAGGGATTTCC68920.1356355960389368No Hit
ACTGGACTTGGAGTCAGAAGGCA68070.1339627832613237No Hit
TATTGCACTTGTCCCGGCCTGTAAT67250.13234901093468512No Hit
TTCACAGTGGCTAAGTTCCGC66760.13138468356876698No Hit
TTCAAGTAATCCAGGATAGGCTT65920.1297315509414787No Hit
AACACCCTGATTGCTCCTATCTGATT65880.12965283034017927No Hit
TGAGGTAGTAGTTTGTGCTGTT64610.12715345124892202No Hit
ACGCCTGTCTGAGCGTCGCT64080.12611040328170442No Hit
ACTGGACTTGGAGTCAGAAGGCT63630.12522479651708573No Hit
ATGCACCTGGGCAAGGATTCTGA63460.12489023396156308No Hit
TGTCAGTTTGTCAAATACCCCA62830.1236503844910969No Hit
TTCACAGTGGCTAAGTTCCG61970.12195789156315892No Hit
TGATGTAGTAGGTTGTATAGTT61610.12124940615146394No Hit
CAACACCAGTCGATGGGCTGT61070.1201866780339215No Hit
CGCCCCACTGCTAAATTTGACTG60210.11849418510598352No Hit
CCCCCACTGCTAAATTTGACTGGCT60170.11841546450468408No Hit
TTCAGGATGGCCGAGCGGTCTAAGG59030.11617192736765002No Hit
TTCAAGTAATCCAGGATAGGC57320.1128066216620989No Hit
GGAATACCGGGTGCTGTAGGCTT55400.10902803279972573No Hit
ACTTGACTTGGAGTCAGAAGGC54370.10700097731626514No Hit
ACCGGGTGCTGTAGGCTT54240.10674513536204194No Hit
CCAAGTCCTTCTGATCGAGGCCC52920.1041473555191604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)