FastQCFastQC Report
Fri 9 Sep 2022
EGAF00003947575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6795245
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG94358313.885930529362811No Hit
CAACGGAATCCCAAAAGCAGCTG6239489.182126619422846No Hit
CCCCCACTGCTAAATTTGACTG2603383.8311790082623953No Hit
TAGCAGCACGTAAATATTGGCG2587233.80741238910444No Hit
AACATTCAACGCTGTCGGTGAGT2241683.298895036161316No Hit
AAGCTGCCAGTTGAAGAACTGT2203063.242061176602168No Hit
TTCAAGTAATCCAGGATAGGCT1920162.8257406465844865No Hit
AACATTCAACGCTGTCGGTGAG1507222.2180510047834923No Hit
TATTGCACTTGTCCCGGCCTGTAT1356331.9959986726012087No Hit
TATTGCACTTGTCCCGGCCTGTAA1351221.9884787082732118No Hit
TAGCTTATCAGACTGATGTTGACT1308171.925125584140086No Hit
TAGCTTATCAGACTGATGTTGAC1194401.7576996855889668No Hit
TGTAAACATCCTTGACTGGAAGCT839891.2359966417693549No Hit
TGAGGTAGTAGGTTGTATAGTT608960.896156062069874No Hit
TAGCTTATCAGACTGATGTTGA602960.8873263583579399No Hit
TATTGCACTTGTCCCGGCCTGT578010.8506095070891484No Hit
CATAAAGTAGAAAGCACTACT552010.8123474576707683No Hit
TATTGCACTTGTCCCGGCCTGTA517270.7612234731786712No Hit
AGCAGCATTGTACAGGGCTATGA495910.7297897279641867No Hit
TGAGGTAGTAGATTGTATAGTT429880.6326188386143546No Hit
TGTAAACATCCCCGACTGGAAGCT427280.6287926336725166No Hit
CAACGGAATCCCAAAAGCAGCTGT373400.5495018943393506No Hit
ACTGGACTTGGAGTCAGAAGGC366770.5397450717376636No Hit
TTCAAGTAATTCAGGATAGGTT350780.5162139113453599No Hit
CCCATAAAGTAGAAAGCACTAC330710.48667855242894115No Hit
TGTCAGTTTGTCAAATACCCCAA282320.41546699199219456No Hit
CATTGCACTTGTCTCGGTCTGA275210.40500379309355294No Hit
CTTCTCACTACTGCACTTGACTAG249550.36724209355218246No Hit
TTCTCACTACTGCACTTGACTAG242230.35646985502362316No Hit
TGTAAACATCCTACACTCTCAGCT223770.32930379993657327No Hit
CACCCGGCTGTGTGCACATGTGC218760.3219309973371085No Hit
CCCATAAAGTAGAAAGCACT210420.30965770917752045No Hit
CAAAGAATTCTCCTTTTGGGCT208270.3064937320140775No Hit
TGAGGTAGTAGTTTGTACAGTT196380.28899620249159524No Hit
CACTAGATTGTGAGCTCCTGGAA191510.2818294263120756No Hit
CACTAGATTGTGAGCTCCTGGA184610.2716752670433516No Hit
CTCCCACTGCTTCACTTGACTAG178320.2624187943186743No Hit
TGTCAGTTTGTCAAATACCCCA174940.2574447278942849No Hit
TATTGCACTTGTCCCGGCCTGTAG174560.2568855133258624No Hit
CCCCCACTGCTAAATTTGACTGGCTT168630.24815882282390114No Hit
AACATTCAACGCTGTCGGTGAGTT166270.2446858060305405No Hit
CCCCCCACTGCTAAATTTGACTGG162350.238917066272077No Hit
TAGCTTATCAGACTGATGTTGACA161730.23800466355517721No Hit
ACCCCCCACTGCTAAATTTGACTG160070.23556177886154217No Hit
CAAAGAATTCTCCTTTTGGGCTT159270.2343844850332843No Hit
CCCCCCACTGCTAAATTTGACTGGCT151550.22302359959059606No Hit
TAGCAGCACGTAAATATTGGC145730.21445878699002022No Hit
TCGTACGACTCTTAGCGG142390.2095435852570437No Hit
TATTGCACTTGTCCCGGCCTGTAAT130700.19234037919162592No Hit
AACGGAATCCCAAAAGCAGCTG129360.19036841202929403No Hit
TATTGCACTTGTCCCGGCCTGTT127690.18791081116280575No Hit
TTCAAGTAATCCAGGATAGGCTT123840.18224508461431485No Hit
TATTGCACTTGTCCCGGCCTGTTT118670.17463682324919852No Hit
CCCCCCACTGCTAAATTTGACTGT117400.17276786929683918No Hit
CAACACCAGTCGATGGGCTGT114120.167940964600982No Hit
TCAGTGCACTACAGAACTTTGT111670.16433550225194235No Hit
TGTAAACATCCCCGACTGGAAGC111540.16414419200485045No Hit
TGAGGTAGTAGTTTGTGCTGTT106800.1571687260724227No Hit
CTCCCACTGCTTCACTTGACTAGCCT101580.14948688384304024No Hit
ACTGGACTTGGAGTCAGAAGGCA101180.14889823692891133No Hit
TATTGCACTTGTCCCGGCCTGTAAA99680.14669081100092787No Hit
ACTGGACTTGGAGTCAGAAGGCT98010.1442332101344396No Hit
AGCTCGGTCTGAGGCCCCTCAGT97870.14402718371449447No Hit
AGCAGCATTGTACAGGGCTATGT97160.14298233544191563No Hit
TGATGTAGTAGGTTGTATAGTT90970.13387302444577054No Hit
CAACGGAATCCCAAAAGCAGCT88890.13081206049230013No Hit
TTATAAAGCAATGAGACTGATT88620.1304147238252631No Hit
TCGTTTCCCGGGCGGCGCACCA86250.12692699085904924No Hit
CAAAGTGCTGTTCGTGCAGGTAG85820.12629419542636064No Hit
TTCAAGTAATCCAGGATAGGC83310.12260043604020163No Hit
TAGCAGCACATCATGGTTTACA82840.12190877591610016No Hit
CCCATAAAGTAGAAAGCACTA82790.12183519505183403No Hit
TTCACAGTGGCTAAGTTCCGC81950.1205990365321633No Hit
ACCACAGGGTAGAACCACGGAC81610.12009868665515372No Hit
CGCCTGTCTGAGCGTCGCT79110.11641964344184794No Hit
ACTGGACTTGGAGTCAGAAGG77560.11413863664959836No Hit
TAGCAGCACGTAAATATTGGCGT77450.1139767587482129No Hit
GCCTGTCTGAGCGTCGCT76790.11300549133990018No Hit
CAGTGCAATAGTATTGTCAAAGC76270.11224025035153258No Hit
ATCACATTGCCAGGGATTTCCA75910.11171046812881655No Hit
TGTCAGTTTGTCAAATACCCC75420.11098937565900861No Hit
TTCACAGTGGCTAAGTTCTGC75320.11084221393047639No Hit
TCAGTGCACTACAGAACTTTGTC73710.10847291010110748No Hit
CCCCCCACTGCTAAATTTGACTGTA72260.10633906503739012No Hit
ATGCACCTGGGCAAGGATTCTGA72070.10605945775317889No Hit
ATCACATTGCCAGGGATTTCC71930.10585343133323376No Hit
TTCACAGTGGCTAAGTTCCG70750.10411692293655343No Hit
ACTTGACTTGGAGTCAGAAGGC69780.10268945416979079No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)