FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6435068
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG74182611.52786575060279No Hit
CAACGGAATCCCAAAAGCAGCTG6186679.613993201004249No Hit
CCCCCACTGCTAAATTTGACTG2564743.985567829275464No Hit
AAGCTGCCAGTTGAAGAACTGT2131163.311790955433571No Hit
TTCAAGTAATCCAGGATAGGCT2075883.2258866572971723No Hit
TAGCAGCACGTAAATATTGGCG2064753.208590802770072No Hit
AACATTCAACGCTGTCGGTGAGT2015593.132196893645879No Hit
AACATTCAACGCTGTCGGTGAG1437462.233791468870259No Hit
TATTGCACTTGTCCCGGCCTGTAA848831.319069200201148No Hit
TAGCTTATCAGACTGATGTTGACT811251.2606704389137768No Hit
TGAGGTAGTAGGTTGTATAGTT803211.2481763984467609No Hit
TAGCTTATCAGACTGATGTTGAC769511.195807099474318No Hit
TATTGCACTTGTCCCGGCCTGTAT740551.1508036900309369No Hit
GGAATACCGGGTGCTGTAGGCTTT639270.9934160757897197No Hit
GCCTGTCTGAGCGTCGCT621950.9665010532911229No Hit
TGTAAACATCCTTGACTGGAAGCT611100.9496403146011821No Hit
CATAAAGTAGAAAGCACTACT561380.8723761738026701No Hit
TATTGCACTTGTCCCGGCCTGT462850.7192620186764149No Hit
TAGCTTATCAGACTGATGTTGA439820.6834737410700245No Hit
TGAGGTAGTAGATTGTATAGTT437200.6794023000223152No Hit
CAACGGAATCCCAAAAGCAGCTGT408100.6341813326603541No Hit
GGAATACCGGGTGCTGTAGGCTT396030.615424732108503No Hit
AGCAGCATTGTACAGGGCTATGA364000.5656505883076915No Hit
CCCATAAAGTAGAAAGCACTAC323130.5021392159336933No Hit
TGTAAACATCCCCGACTGGAAGCT312010.48485890125791986No Hit
TATTGCACTTGTCCCGGCCTGTA309990.4817198512898388No Hit
TTCAAGTAATTCAGGATAGGTT298940.46454831557335524No Hit
ACTGGACTTGGAGTCAGAAGGC269550.4188766925229073No Hit
TGAATACCGGGTGCTGTAGGCTTT257650.4003842694436174No Hit
CCCATAAAGTAGAAAGCACT240660.37398206203881607No Hit
TTCTCACTACTGCACTTGACTAG239960.37289427244591666No Hit
TCCTGTCTGAGCGTCGCT235370.36576148068676195No Hit
CATTGCACTTGTCTCGGTCTGA227920.35418429144804686No Hit
CCCCCACTGCTAAATTTGACTGGCTT226280.3516357558303968No Hit
TGAGGTAGTAGTTTGTACAGTT213690.33207108300953464No Hit
CTTCTCACTACTGCACTTGACTAG207090.32181478113362594No Hit
CACCCGGCTGTGTGCACATGTGC196530.305404698132172No Hit
TGTAAACATCCTACACTCTCAGCT189900.29510177670228194No Hit
CAAAGAATTCTCCTTTTGGGCT177880.2764228754070664No Hit
CTCCCACTGCTTCACTTGACTAG175000.2719473982248517No Hit
TCGTACGACTCTTAGCGG174040.2704555724974468No Hit
TGATGTAGTAGGTTGTATAGTT158740.2466795999669312No Hit
TGAATACCGGGTGCTGTAGGCTT156290.24287233639178327No Hit
CCCCCCACTGCTAAATTTGACTGGCT153310.23824146069629723No Hit
TGTCAGTTTGTCAAATACCCCAA146500.2276588219425187No Hit
ACCCCCCACTGCTAAATTTGACTG145880.22669535116023637No Hit
CGCCTGTCTGAGCGTCGCT143640.22321442446295828No Hit
CAACACCAGTCGATGGGCTGT141430.2197801173196616No Hit
AACCGGGCGGAAACACCA141200.21942270073913747No Hit
AACGGAATCCCAAAAGCAGCTG140250.21794641486305974No Hit
AACATTCAACGCTGTCGGTGAGTT133050.20675772190752298No Hit
CCCCCCACTGCTAAATTTGACTGG132340.20565439246329645No Hit
CAAAGAATTCTCCTTTTGGGCTT124690.19376640619803862No Hit
ACCGGGTGCTGTAGGCTT123600.19207256240338097No Hit
TAGCAGCACGTAAATATTGGC122940.1910469322157901No Hit
TGAGGTAGTAGTTTGTGCTGTT122510.19037871860872332No Hit
TTAATGCTAATCGTGATAGGGGTT120510.18727074834329646No Hit
CTCCCACTGCTTCACTTGACTAGCCT114970.17866167070806402No Hit
TGTCAGTTTGTCAAATACCCCA104190.16190971097741313No Hit
CACTAGATTGTGAGCTCCTGGA102470.15923685654914604No Hit
TTCAAGTAATCCAGGATAGGCTT98210.15261687988378678No Hit
TAGCTTATCAGACTGATGTTGACA94260.1464786386095687No Hit
GGAATACCGGGTGCTGTAGGCTTA93590.1454374685706507No Hit
CACTAGATTGTGAGCTCCTGGAA93200.14483141436889246No Hit
CAACGGAATCCCAAAAGCAGCT93000.14452061734234978No Hit
TATTGCACTTGTCCCGGCCTGTAG91520.1422207193459339No Hit
TTCAAGTAATCCAGGATAGGC86480.13438863427705816No Hit
TGATGTAGTAGATTGTATAGTT85850.13340962364344866No Hit
TTCACAGTGGCTAAGTTCCGC85380.13267925063107336No Hit
TGTAAACATCCCCGACTGGAAGC84260.13093878728243433No Hit
TATTGCACTTGTCCCGGCCTGTT83680.13003747590546053No Hit
AGCTCGGTCTGAGGCCCCTCAGT82200.12773757790904464No Hit
ACCGGGTGCTGTAGGCTTA81090.1260126544117327No Hit
ACCGGGTGCTGTAGGCTTT80850.1256396979798815No Hit
ACTGGACTTGGAGTCAGAAGGCT79310.1232465608755028No Hit
CTACGCCTGTCTGAGCGTCGCT78650.12222093068791193No Hit
CCCATAAAGTAGAAAGCACTA76270.11852244607205394No Hit
ATCACATTGCCAGGGATTTCC75850.11786977231631429No Hit
TATTGCACTTGTCCCGGCCTGTAAT74490.11575635253582402No Hit
TGAGGTAGTAGGTTGTGTGGTT72370.11246190405447153No Hit
AACATTCAACGCTGTCGGTGA71890.11171599119076908No Hit
TTCACAGTGGCTAAGTTCTGC71800.11157613252882487No Hit
TTCACAGTGGCTAAGTTCCG71060.11042618353061694No Hit
ATCCCGGACGAGCCCCCA70930.1102241654633642No Hit
ACCACAGGGTAGAACCACGGAC69570.1081107456828739No Hit
TTATAAAGCAATGAGACTGATT69080.10734929296784433No Hit
ATGCACCTGGGCAAGGATTCTGA68690.10674323876608607No Hit
CCCCCCACTGCTAAATTTGACTGT68650.10668107936077754No Hit
AGAGGTAGTAGGTTGCATAGTT67590.1050338551201013No Hit
ACTGGACTTGGAGTCAGAAGGCA65270.10142860961220611No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)