FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10579277
Sequences flagged as poor quality0
Sequence length18-26
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG115211210.890271613079042No Hit
CAACGGAATCCCAAAAGCAGCTG7963047.5270172054290665No Hit
TAGCAGCACGTAAATATTGGCG4096183.871890300253978No Hit
CCCCCACTGCTAAATTTGACTG3470263.2802430638691096No Hit
AAGCTGCCAGTTGAAGAACTGT3335663.1530131974046998No Hit
AACATTCAACGCTGTCGGTGAGT3071682.9034876390891364No Hit
TTCAAGTAATCCAGGATAGGCT3067382.8994230891203623No Hit
AACATTCAACGCTGTCGGTGAG2123592.0073110856252274No Hit
TAGCTTATCAGACTGATGTTGACT2110141.9945975514205745No Hit
TAGCTTATCAGACTGATGTTGAC2045081.9330999651488472No Hit
TATTGCACTTGTCCCGGCCTGTAT1966851.8591535130425263No Hit
TATTGCACTTGTCCCGGCCTGTAA1918551.8134982192072293No Hit
TGTAAACATCCTTGACTGGAAGCT1521951.4386143779012497No Hit
TGAGGTAGTAGGTTGTATAGTT1129041.0672184876149853No Hit
CATAAAGTAGAAAGCACTACT1059691.001665803816272No Hit
TAGCTTATCAGACTGATGTTGA1034350.9777133163258699No Hit
TATTGCACTTGTCCCGGCCTGT945120.8933691782529184No Hit
TGAGGTAGTAGATTGTATAGTT853110.8063972613629458No Hit
TATTGCACTTGTCCCGGCCTGTA779790.7370919581744575No Hit
AGCAGCATTGTACAGGGCTATGA736140.6958320497705088No Hit
TGTAAACATCCCCGACTGGAAGCT674160.6372458155694383No Hit
TTCAAGTAATTCAGGATAGGTT623480.5893408405886338No Hit
ACTGGACTTGGAGTCAGAAGGC566340.5353295882128807No Hit
CCCATAAAGTAGAAAGCACTAC515380.4871599448620165No Hit
CAACGGAATCCCAAAAGCAGCTGT499660.4723007063715224No Hit
CATTGCACTTGTCTCGGTCTGA423330.40015021820489244No Hit
TGTCAGTTTGTCAAATACCCCAA418290.395386187543818No Hit
TGAGGTAGTAGTTTGTACAGTT387770.3665373352073114No Hit
TTCTCACTACTGCACTTGACTAG384420.36337076720838296No Hit
TGTAAACATCCTACACTCTCAGCT373000.35257607868666263No Hit
CAAAGAATTCTCCTTTTGGGCT364960.34497631548923424No Hit
CTTCTCACTACTGCACTTGACTAG353740.3343706758032709No Hit
CACCCGGCTGTGTGCACATGTGC335360.3169970877972096No Hit
CCCATAAAGTAGAAAGCACT312440.29533209121946613No Hit
TGTCAGTTTGTCAAATACCCCA295700.2795087036666116No Hit
CCCCCACTGCTAAATTTGACTGGCTT290960.27502824625917255No Hit
CACTAGATTGTGAGCTCCTGGAA274490.25946007463458987No Hit
TAGCTTATCAGACTGATGTTGACA264910.2504046354018332No Hit
CACTAGATTGTGAGCTCCTGGA264410.24993201331244091No Hit
TATTGCACTTGTCCCGGCCTGTAG259070.24488440939773107No Hit
CAAAGAATTCTCCTTTTGGGCTT257420.2433247565027364No Hit
CTCCCACTGCTTCACTTGACTAG250960.2372184791077878No Hit
ACCCCCCACTGCTAAATTTGACTG248650.23503496505479535No Hit
AACATTCAACGCTGTCGGTGAGTT227030.21459878590947187No Hit
TCAGTGCACTACAGAACTTTGT225790.213426683127779No Hit
TAGCAGCACGTAAATATTGGC225590.21323763429202205No Hit
CCCCCCACTGCTAAATTTGACTGG221750.2096078966454891No Hit
CCCCCCACTGCTAAATTTGACTGGCT221580.20944720513509574No Hit
TCGTACGACTCTTAGCGG214390.2026508994896343No Hit
AGCTCGGTCTGAGGCCCCTCAGT213320.20163948821833474No Hit
TGAGGTAGTAGTTTGTGCTGTT211260.19969228521003846No Hit
TATTGCACTTGTCCCGGCCTGTT207480.1961192622142326No Hit
AACGGAATCCCAAAAGCAGCTG196460.18570267136402613No Hit
CAACACCAGTCGATGGGCTGT194530.1838783500989718No Hit
TGAGAACTGAATTCCATAGGCTGT193230.1826495326665518No Hit
TGTAAACATCCCCGACTGGAAGC191190.18072123454183117No Hit
TTCAAGTAATCCAGGATAGGCTT190400.17997449164059132No Hit
TATTGCACTTGTCCCGGCCTGTTT183980.17390602401279406No Hit
TGAGAACTGAATTCCATGGGTT183650.17359409343379514No Hit
TATTGCACTTGTCCCGGCCTGTAAT183470.1734239494816139No Hit
TTAATGCTAATCGTGATAGGGGTT172900.1634327185118605No Hit
ACTGGACTTGGAGTCAGAAGGCA159630.1508893282593886No Hit
CGCCTGTCTGAGCGTCGCT159450.1507191843072074No Hit
ACCGGGTGCTGTAGGCTTA157240.14863019467209337No Hit
CCCCCCACTGCTAAATTTGACTGT156320.14776057002761153No Hit
TATTGCACTTGTCCCGGCCTGTAAA154640.14617255980725335No Hit
ACTGGACTTGGAGTCAGAAGGCT153460.14505717167628754No Hit
TCGTTTCCCGGGCGGCGCACCA152640.14428207144968414No Hit
CTCCCACTGCTTCACTTGACTAGCCT152610.1442537141243206No Hit
ATCCCGGACGAGCCCCCA151650.14334627971268735No Hit
TTATAAAGCAATGAGACTGATT148010.13990559090191135No Hit
AGCAGCATTGTACAGGGCTATGT147750.13965982741542735No Hit
ACCGGGTGCTGTAGGCTT147160.13910213334994442No Hit
GGAATACCGGGTGCTGTAGGCTTT146140.13813798428758411No Hit
TGTCAGTTTGTCAAATACCCC145790.1378071488250095No Hit
GACCCGCGGGCGCTCTCCAGTCCT140160.1324854240984521No Hit
TTCACAGTGGCTAAGTTCCGC139020.13140784573463762No Hit
TCAGTGCACTACAGAACTTTGTC136050.12860047052364731No Hit
TTCAAGTAATCCAGGATAGGC135000.12760796413592346No Hit
ACTGGACTTGGAGTCAGAAGG129340.12225788208400253No Hit
TTCACAGTGGCTAAGTTCCG127940.12093454023370405No Hit
CCCATAAAGTAGAAAGCACTA126610.1196773654759205No Hit
TAGCAGCACGTAAATATTGGCGT123170.11642572550090143No Hit
ATCACATTGCCAGGGATTTCCA123100.11635955840838652No Hit
CAAAGTGCTGTTCGTGCAGGTAG121000.11437454563293882No Hit
TTCACAGTGGCTAAGTTCTGC120360.11376958935851665No Hit
CAGTGCAATAGTATTGTCAAAGC120110.11353327831382051No Hit
TAGCAGCACATCATGGTTTACA118060.11159552774731203No Hit
TGTAAACATCCTACACTCAGCT116660.11027218589701358No Hit
ATGCACCTGGGCAAGGATTCTGA116080.10972394427331848No Hit
ATCACATTGCCAGGGATTTCC115530.10920405997498694No Hit
CAACGGAATCCCAAAAGCAGCT114880.10858965125877694No Hit
ACCGGGTGCTGTAGGCTTT114060.10781455103217356No Hit
CTACGCCTGTCTGAGCGTCGCT110060.10403357431703508No Hit
GGAATACCGGGTGCTGTAGGCTT109750.10374054862161185No Hit
TGTAAACATCCTTGACTGGAAGC108410.10247392142204047No Hit
ACCACAGGGTAGAACCACGGAC106980.10112222224637846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)