FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10018885
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG102232610.203989765328178No Hit
CAACGGAATCCCAAAAGCAGCTG8191228.175780039395601No Hit
CCCCCACTGCTAAATTTGACTG3611913.6051017653162No Hit
AACATTCAACGCTGTCGGTGAGT3544863.5381781505626626No Hit
TAGCAGCACGTAAATATTGGCG3006093.000423699842847No Hit
TTCAAGTAATCCAGGATAGGCT2804602.7993134964619317No Hit
AAGCTGCCAGTTGAAGAACTGT2704762.699661688900511No Hit
AACATTCAACGCTGTCGGTGAG2348662.3442329161378734No Hit
TATTGCACTTGTCCCGGCCTGTAA1381001.3783968974591485No Hit
TATTGCACTTGTCCCGGCCTGTAT1324271.321773830121815No Hit
TCGTACGACTCTTAGCGG1310841.308369144869913No Hit
TAGCTTATCAGACTGATGTTGAC1109251.107159129983027No Hit
TATTGCACTTGTCCCGGCCTGT1099811.0977369238193673No Hit
TAGCTTATCAGACTGATGTTGACT998570.9966877551743532No Hit
CATAAAGTAGAAAGCACTACT884820.8831521671323705No Hit
TAGCTTATCAGACTGATGTTGA802290.8007777312545258No Hit
TGAGGTAGTAGGTTGTATAGTT780960.7794879370309171No Hit
TATTGCACTTGTCCCGGCCTGTA759590.75815821820492No Hit
TGTAAACATCCTTGACTGGAAGCT670760.6694956574509039No Hit
TTCAAGTAATTCAGGATAGGTT592040.5909240399505534No Hit
AGCAGCATTGTACAGGGCTATGA555330.554283236108609No Hit
CAACGGAATCCCAAAAGCAGCTGT504450.5034991418705774No Hit
TGAGGTAGTAGATTGTATAGTT463450.4625764244224781No Hit
CATTGCACTTGTCTCGGTCTGA430180.4293691363859352No Hit
TGTAAACATCCCCGACTGGAAGCT400400.39964526990777915No Hit
CCCATAAAGTAGAAAGCACT390700.3899635538285947No Hit
CCCATAAAGTAGAAAGCACTAC381660.38094059368881866No Hit
CAACGGAATCCCAAAAGCAGCT380480.3797628179183612No Hit
ACTGGACTTGGAGTCAGAAGGC370150.36945228935155955No Hit
CACCCGGCTGTGTGCACATGTGC352470.3518056150958914No Hit
TTCTCACTACTGCACTTGACTAG350100.3494400824043793No Hit
TTAATGCTAATCGTGATAGGGGTT320910.3203051038114521No Hit
ATCCCGGACGAGCCCCCA308080.30749928759537615No Hit
GCCTGTCTGAGCGTCGCT307930.3073495703364197No Hit
ACCGGGTGCTGTAGGCTT305500.304924150741325No Hit
CTTCTCACTACTGCACTTGACTAG300970.3004026895208399No Hit
CGCCTGTCTGAGCGTCGCT283970.28343473350577436No Hit
CAACACCAGTCGATGGGCTGT276040.2755196810822761No Hit
CTCCCACTGCTTCACTTGACTAG264870.26437073586531834No Hit
AACATTCAACGCTGTCGGTGAGTT253720.25324175294955475No Hit
TGAGGTAGTAGTTTGTACAGTT234580.23413783070671038No Hit
ACCGGGTGCTGTAGGCTTA232750.23231128014744154No Hit
CTACGCCTGTCTGAGCGTCGCT220100.21968512464211337No Hit
TGATGTAGTAGGTTGTATAGTT219720.21930584091942365No Hit
CGTTCGCGCTTTCCCCTG216750.21634143919208576No Hit
TGTCAGTTTGTCAAATACCCCAA216230.21582241936103666No Hit
CAAAGAATTCTCCTTTTGGGCT209860.20946442643068564No Hit
ACCGGGTGCTGTAGGCTTT207750.2073584036546981No Hit
TACGCCTGTCTGAGCGTCGCT206790.20640021319737675No Hit
TGTAAACATCCTACACTCTCAGCT206230.2058412687639393No Hit
TATTGCACTTGTCCCGGCCTGTT203690.20330605651227657No Hit
TCCTGTCTGAGCGTCGCT195670.19530117373340447No Hit
CACTAGATTGTGAGCTCCTGGA193000.19263620652397948No Hit
TAGCAGCACGTAAATATTGGC187790.18743602706289172No Hit
ACCCCCCACTGCTAAATTTGACTG183940.18359328408300923No Hit
CCCCCCACTGCTAAATTTGACTGG180990.1806488446568655No Hit
TTCACAGTGGCTAAGTTCCG180510.18016974942820482No Hit
TCGTTTCCCGGCCAATGCACCA178100.17776429213430436No Hit
CCCCCACTGCTAAATTTGACTGGCTT171260.17093718512588973No Hit
TATTGCACTTGTCCCGGCCTGTAG169750.16943003138572807No Hit
AACGGAATCCCAAAAGCAGCTG169460.16914057801841223No Hit
CACTAGATTGTGAGCTCCTGGAA151500.15121443154602535No Hit
TGAGGTAGTAGTTTGTGCTGTT148490.14821010521629902No Hit
TGTCAGTTTGTCAAATACCCCA146950.14667300802434602No Hit
CCCATAAAGTAGAAAGCACTA144410.1441377957726833No Hit
CAAAGAATTCTCCTTTTGGGCTT139020.1387579556008478No Hit
CCCCCCACTGCTAAATTTGACTGGCT137980.13771991593874966No Hit
ATCCCACCGCTGCCACCA137100.1368415746862051No Hit
TTCAAGTAATCCAGGATAGGC133930.13367754994692524No Hit
TCGTTTCCCGGGCGGCGCACCA132540.13229017001392868No Hit
TGATGTAGTAGATTGTATAGTT130290.1300444111295818No Hit
TTCAAGTAATCCAGGATAGGCTT129900.12965514625629498No Hit
TGAGAACTGAATTCCATGGGTT127240.12700016019746707No Hit
ACTTGACTTGGAGTCAGAAGGC125820.12558283681267926No Hit
CCCCCCACTGCTAAATTTGACTGT125060.12482426936729984No Hit
TAGCTTATCAGACTGATGTTGACA121460.12123105515234478No Hit
ACCACAGGGTAGAACCACGGAC120700.1204724877069654No Hit
TGTAAACATCCCCGACTGGAAGC120170.11994348672531924No Hit
AGCTCGGTCTGAGGCCCCTCAGT118580.11835648378038074No Hit
TTCACAGTGGCTAAGTTCTG117250.1170289907509668No Hit
TATTGCACTTGTCCCGGCCTGTTT115800.11558172391438767No Hit
AACATTCAACGCTGTCGGTGA115140.11492296797497925No Hit
TTATAAAGCAATGAGACTGATT114920.1147033826618431No Hit
AGCAGCATTGTACAGGGCTATGT111860.11164915057913131No Hit
TATTGCACTTGTCCCGGCCTGA104990.10479210011892541No Hit
ACTGGACTTGGAGTCAGAAGGCA102720.1025263789333843No Hit
CAAAGTGCTGTTCGTGCAGGT102600.10240660512621914No Hit
TCAGTGCACTACAGAACTTTGT101850.10165801883143682No Hit
ACTGGACTTGGAGTCAGAAGGCT101390.10119888590397035No Hit
CAAAGTGCTGTTCGTGCAGGTAG100370.10018080854306641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)