FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14497959
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG180492012.44947650907276No Hit
CAACGGAATCCCAAAAGCAGCTG12916788.909378209719037No Hit
CCCCCACTGCTAAATTTGACTG7230194.987039899892116No Hit
AACATTCAACGCTGTCGGTGAGT5702953.9336226568167287No Hit
TAGCAGCACGTAAATATTGGCG4948743.413404604054957No Hit
TTCAAGTAATCCAGGATAGGCT4017072.7707831150577817No Hit
AACATTCAACGCTGTCGGTGAG3991382.7530633794729313No Hit
AAGCTGCCAGTTGAAGAACTGT3312172.2845767462854596No Hit
TAGCTTATCAGACTGATGTTGACT2410451.6626133375049552No Hit
TGAGGTAGTAGGTTGTATAGTT2387271.6466248800951913No Hit
TAGCTTATCAGACTGATGTTGAC2200171.5175722320638374No Hit
TATTGCACTTGTCCCGGCCTGTAA2048881.4132196124985592No Hit
TATTGCACTTGTCCCGGCCTGTAT1635021.1277587417649615No Hit
TAGCTTATCAGACTGATGTTGA1534271.0582662014701518No Hit
TGAGGTAGTAGATTGTATAGTT1336590.9219159745175166No Hit
TGTAAACATCCTTGACTGGAAGCT1212720.8364763619486025No Hit
TATTGCACTTGTCCCGGCCTGT1014610.6998295415237413No Hit
AGCAGCATTGTACAGGGCTATGA1011930.6979810054642863No Hit
TTCAAGTAATTCAGGATAGGTT896310.6182318490485453No Hit
TGTAAACATCCCCGACTGGAAGCT872080.6015191517647415No Hit
CATAAAGTAGAAAGCACTACT866860.5979186449623702No Hit
TATTGCACTTGTCCCGGCCTGTA812490.5604168145323076No Hit
ACTGGACTTGGAGTCAGAAGGC804630.5549953617609209No Hit
CAACGGAATCCCAAAAGCAGCTGT674900.4655138009426016No Hit
CATTGCACTTGTCTCGGTCTGA611370.4216938398018645No Hit
TGTCAGTTTGTCAAATACCCCAA586990.40487767967891203No Hit
TTCTCACTACTGCACTTGACTAG568570.3921724430314639No Hit
CACCCGGCTGTGTGCACATGTGC550760.37988795526322017No Hit
TGAGGTAGTAGTTTGTACAGTT522690.3605266092972121No Hit
CCCATAAAGTAGAAAGCACT485070.3345781292387432No Hit
CTTCTCACTACTGCACTTGACTAG470190.324314615595202No Hit
TGATGTAGTAGGTTGTATAGTT415220.2863989338085451No Hit
CAACACCAGTCGATGGGCTGT397260.2740109832011527No Hit
AACATTCAACGCTGTCGGTGAGTT389880.26892061151504154No Hit
CCCCCACTGCTAAATTTGACTGGCTT372780.25712584785210113No Hit
TAGCAGCACGTAAATATTGGC361460.24931785225768677No Hit
CCCATAAAGTAGAAAGCACTAC360920.24894538603675181No Hit
CCCCCCACTGCTAAATTTGACTGG328350.22648015489628573No Hit
AACGGAATCCCAAAAGCAGCTG328070.22628702426320835No Hit
CAAAGAATTCTCCTTTTGGGCT318300.21954814467332953No Hit
TGTCAGTTTGTCAAATACCCCA309770.21366455788707917No Hit
ACCCCCCACTGCTAAATTTGACTG303430.20929152855239833No Hit
TGAGGTAGTAGTTTGTGCTGTT294040.20281475482169595No Hit
CTCCCACTGCTTCACTTGACTAG288820.19921424801932464No Hit
TGTAAACATCCTACACTCTCAGCT287270.19814513201478912No Hit
CCCGCCACTGCTAAATTTGACTG282750.1950274517951113No Hit
TAGCTTATCAGACTGATGTTGACA273210.1884472152252603No Hit
GACCCGCGGGCGCTCTCCAGTCCT270400.18650901137187656No Hit
GGAATACCGGGTGCTGTAGGCTTT266730.1839776205740408No Hit
CAACGGAATCCCAAAAGCAGCT255470.17621101011528587No Hit
CAAAGAATTCTCCTTTTGGGCTT254730.17570059344215277No Hit
CACTAGATTGTGAGCTCCTGGA251990.17381067224703836No Hit
ACTGGACTTGGAGTCAGAAGGCT251120.17321058777997647No Hit
TTCAAGTAATCCAGGATAGGCTT245110.16906517669142257No Hit
TATTGCACTTGTCCCGGCCTGTAG235380.16235388719198338No Hit
TGTAAACATCCCCGACTGGAAGC235300.1622987070111041No Hit
TGATGTAGTAGATTGTATAGTT231290.15953280044453155No Hit
CCCCCCACTGCTAAATTTGACTGGCT225200.15533220917509838No Hit
TGAGAACTGAATTCCATAGGCTGT223290.15401478235660618No Hit
CACTAGATTGTGAGCTCCTGGAA222780.15366300870350097No Hit
TTCACAGTGGCTAAGTTCTG222120.15320777221124712No Hit
GGAATACCGGGTGCTGTAGGCTT221850.15302153910077965No Hit
CGCCTGTCTGAGCGTCGCT215440.14860022710782944No Hit
TCGTACGACTCTTAGCGG206130.14217863355800633No Hit
AGAATTACTCTGAGACCT204750.14122677543783924No Hit
TTATAAAGCAATGAGACTGATT204400.1409853621464925No Hit
ACTGGACTTGGAGTCAGAAGG199510.1376124735902481No Hit
TATTGCACTTGTCCCGGCCTGTT199000.13726069993714288No Hit
TCAGTGCACTACAGAACTTTGT197200.13601914586735966No Hit
AGCAGCATTGTACAGGGCTATGT196180.1353155985611492No Hit
ACTGGACTTGGAGTCAGAAGGCA193590.13352914020518336No Hit
TCAGTGCACTACAGAACTTTGTC191280.1319358124822949No Hit
CAAGGGAATCCCAAAAGCAGCTG190700.13153575617092034No Hit
AACATTCAACGCTGTCGGTGA182640.12597635294733556No Hit
GTCAGGATGGCCGAGCGGTCTAAGGC181490.12518313784719629No Hit
TTCAAGTAATCCAGGATAGGC181450.12515554775675666No Hit
TGAGGTAGTAGGTTGTGTGGTT178450.12308629097378465No Hit
ACCGGGTGCTGTAGGCTT177220.12223789569276614No Hit
CTCCCACTGCTTCACTTGACTAGCCT175870.12130673014042874No Hit
TGAGGTAGTAGGTTGTATAGT175730.12121016482389005No Hit
CCCCCCACTGCTAAATTTGACTGT172430.11893398236262084No Hit
AGCAGCATTGTACAGGGCTATG171660.11840287312165802No Hit
AGAGGTAGTAGGTTGCATAGTT168830.11645087422305442No Hit
ACCACAGGGTAGAACCACGGAC168000.11587837984643218No Hit
TATTGCACTTGTCCCGGCCTGTAAT166110.11457474807315982No Hit
TTCACAGTGGCTAAGTTCTGC165470.11413330662612578No Hit
AGCTCGGTCTGAGGCCCCTCAGT164740.1136297874756026No Hit
CGCGACCTCAGATCAGACGTGGCG162380.11200197213966463No Hit
TTCACAGTGGCTAAGTTCCG162280.11193299691356555No Hit
TATTGCACTTGTCCCGGCCTGTAAA156940.1082497198398754No Hit
CCCCCCACTGCTAAATTTGACTGTA149660.10322832337986335No Hit
GGGAATACCGGGTGCTGTAGGCTT148370.10233854296318537No Hit
CCCATAAAGTAGAAAGCACTA146720.10120045173255077No Hit
CAAAGTGCTGTTCGTGCAGGTAG146610.1011245789838418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)