FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10353556
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG119107311.503999205683535No Hit
CCCCCCACTGCTAAATTTGACTG7819267.552245817765413No Hit
TAGCAGCACGTAAATATTGGCG4481154.328126491033612No Hit
TTCAAGTAATCCAGGATAGGCT3739483.6117832366000626No Hit
AACATTCAACGCTGTCGGTGAGT3596293.4734829270252656No Hit
AAGCTGCCAGTTGAAGAACTGT3406733.290396072615051No Hit
AACATTCAACGCTGTCGGTGAG2346462.26633245621118No Hit
CCCCCACTGCTAAATTTGACTG2246412.169698990375867No Hit
TATTGCACTTGTCCCGGCCTGTAA2072442.0016697644751233No Hit
TATTGCACTTGTCCCGGCCTGTAT1922871.8572073208470596No Hit
TAGCTTATCAGACTGATGTTGAC1755951.6959873496603484No Hit
TAGCTTATCAGACTGATGTTGACT1621821.5664376567818823No Hit
TGTAAACATCCTTGACTGGAAGCT1197151.1562694015466763No Hit
TGAGGTAGTAGGTTGTATAGTT1077841.0410336313436659No Hit
TAGCTTATCAGACTGATGTTGA1000500.9663346583531301No Hit
TATTGCACTTGTCCCGGCCTGT1000270.9661125124546581No Hit
CATAAAGTAGAAAGCACTACT801460.7740915295189401No Hit
TATTGCACTTGTCCCGGCCTGTA758410.7325116124353797No Hit
AGCAGCATTGTACAGGGCTATGA750120.7245047015730633No Hit
CAACGGAATCCCAAAAGCAGCTGT700010.6761058712581455No Hit
ACTGGACTTGGAGTCAGAAGGC649890.6276973824259027No Hit
TGAGGTAGTAGATTGTATAGTT629610.6081099092910687No Hit
GCCTGTCTGAGCGTCGCT613010.5920767705317863No Hit
TGTAAACATCCCCGACTGGAAGCT595240.5749135852454944No Hit
CCCATAAAGTAGAAAGCACTAC566370.5470294457285979No Hit
TTCAAGTAATTCAGGATAGGTT551150.5323291823601476No Hit
TGTAAACATCCTACACTCTCAGCT446630.4313783592806182No Hit
CTTCTCACTACTGCACTTGACTAG425680.4111437654850179No Hit
CATTGCACTTGTCTCGGTCTGA398290.3846890865322021No Hit
TTCTCACTACTGCACTTGACTAG378830.3658936118180073No Hit
CACCCGGCTGTGTGCACATGTGC366050.35355002667682484No Hit
CTCCCACTGCTTCACTTGACTAG362460.3500826189571969No Hit
CCCATAAAGTAGAAAGCACT325450.31433644633785723No Hit
CCCCCACTGCTAAATTTGACTGGCTT305380.2949518020668454No Hit
TGAGGTAGTAGTTTGTACAGTT305070.2946523880297745No Hit
CAAAGAATTCTCCTTTTGGGCT271900.26261508606318446No Hit
CAACGGAATCCCAAAAGCAGCT267130.25800797329922204No Hit
AACATTCAACGCTGTCGGTGAGTT266050.2569648534281362No Hit
CCCCCCACTGCTAAATTTGACTGGCT252680.24405141576478653No Hit
AACGGAATCCCAAAAGCAGCTG246790.23836254906043874No Hit
TAGCTTATCAGACTGATGTTGACA241600.23334977856883182No Hit
TATTGCACTTGTCCCGGCCTGTAG226020.21830180857668613No Hit
TAGCAGCACGTAAATATTGGC220850.21330835511972893No Hit
CCCCCCACTGCTAAATTTGACTGG219780.21227489376596792No Hit
TGTCAGTTTGTCAAATACCCCAA218870.21139596868940488No Hit
TATTGCACTTGTCCCGGCCTGTT209190.2020465239189318No Hit
CTCCCACTGCTTCACTTGACTAGCCT202700.19577814617509193No Hit
CGCCTGTCTGAGCGTCGCT199310.19250390880196136No Hit
TAGCAGCACATCATGGTTTACA194250.18761669903557773No Hit
CAAAGAATTCTCCTTTTGGGCTT192510.18593611702105056No Hit
TTCAAGTAATCCAGGATAGGCTT191880.1853276304295838No Hit
TGAGGTAGTAGTTTGTGCTGTT190950.184429388318371No Hit
CACTAGATTGTGAGCTCCTGGA190020.1835311462071582No Hit
TGTCAGTTTGTCAAATACCCCA189200.18273914778651895No Hit
CACTAGATTGTGAGCTCCTGGAA180700.17452940806038042No Hit
CTACGCCTGTCTGAGCGTCGCT177160.17111029292737684No Hit
CAACACCAGTCGATGGGCTGT177100.17105234182342763No Hit
ACTGGACTTGGAGTCAGAAGGCA175160.16917858946240305No Hit
ACCCCCCACTGCTAAATTTGACTG171910.16603957133182068No Hit
ACTGGACTTGGAGTCAGAAGGCT170570.16474533001028827No Hit
TATTGCACTTGTCCCGGCCTGTTT169420.16363460051792833No Hit
TTCACAGTGGCTAAGTTCCGC167990.1622534325404721No Hit
TGTAAACATCCTACACTCAGCT167100.16139382449855874No Hit
AGCAGCATTGTACAGGGCTATGT164170.1585638789223722No Hit
TGTAAACATCCCCGACTGGAAGC160470.1549902275121707No Hit
TATTGCACTTGTCCCGGCCTGTAAT153150.1479201928303667No Hit
TAGCAGCACGTAAATATTGGCGT150050.14492605245965734No Hit
TTCACAGTGGCTAAGTTCTGC147200.1421733750220697No Hit
TCCTGTCTGAGCGTCGCT143680.13877357692371586No Hit
ATCACATTGCCAGGGATTTCC139440.13467836557797147No Hit
TTCAAGTAATCCAGGATAGGC139200.13444656116217463No Hit
CAAAGTGCTGTTCGTGCAGGTAG131090.12661350361170598No Hit
TTCACAGTGGCTAAGTTCCG131000.12652657695578215No Hit
AGCTCGGTCTGAGGCCCCTCAGT127260.12291429147628119No Hit
ACCGGGTGCTGTAGGCTT123800.11957244448187657No Hit
ACTGGACTTGGAGTCAGAAGG123190.11898327492505956No Hit
ATCACATTGCCAGGGATTTCCA121640.1174862047397049No Hit
GGAATACCGGGTGCTGTAGGCTTT120970.11683908407893867No Hit
TATTGCACTTGTCCCGGCCTGTAAA119940.11584425679447719No Hit
GTCAGGATGGCCGAGCGGTCTAAGG119900.1158056227251777No Hit
AACCGGGCGGAAACACCA119450.11537098944555861No Hit
TGAGGTAGTAGGTTGTGTGGTT119190.11511986799511201No Hit
CCCATAAAGTAGAAAGCACTA115490.11154621658491053No Hit
ACCACAGGGTAGAACCACGGAC113550.10967246422388599No Hit
TGATGTAGTAGGTTGTATAGTT113530.10965314718923624No Hit
CCTTGGCTTGGCTCTTGACGTGGACA110750.1069680793729227No Hit
ACCGGGTGCTGTAGGCTTA110140.10637890981610569No Hit
AACATTCAACGCTGTCGGTGAGA108300.10460174262832982No Hit
TTATAAAGCAATGAGACTGATT104940.10135648080717388No Hit
ATGCACCTGGGCAAGGATTCTGA104290.10072867718105741No Hit
GGAATACCGGGTGCTGTAGGCTT103740.10019745872818962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)