FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12512084
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG145440011.62396288260213No Hit
CCCCCCACTGCTAAATTTGACTG7155745.719063267158372No Hit
TAGCAGCACGTAAATATTGGCG5132484.102018496678891No Hit
TTCAAGTAATCCAGGATAGGCT4671843.7338624005401497No Hit
AACATTCAACGCTGTCGGTGAGT3751742.998493296560349No Hit
AAGCTGCCAGTTGAAGAACTGT3698912.9562701145548576No Hit
AACATTCAACGCTGTCGGTGAG2942632.351830438478514No Hit
CCCCCACTGCTAAATTTGACTG2452801.9603448953827356No Hit
TATTGCACTTGTCCCGGCCTGTAA1961101.5673647971033444No Hit
TAGCTTATCAGACTGATGTTGAC1898901.5176528546323698No Hit
TATTGCACTTGTCCCGGCCTGTAT1844281.4739990556329385No Hit
TGAGGTAGTAGGTTGTATAGTT1446231.1558666006398295No Hit
TAGCTTATCAGACTGATGTTGACT1442291.1527176447984204No Hit
TATTGCACTTGTCCCGGCCTGT1416921.1324412463982818No Hit
TAGCTTATCAGACTGATGTTGA1313371.0496812521399312No Hit
TGTAAACATCCTTGACTGGAAGCT1100050.8791900693761326No Hit
CATAAAGTAGAAAGCACTACT984190.7865915861818062No Hit
TTCAAGTAATTCAGGATAGGTT881620.7046148347469534No Hit
TGAGGTAGTAGATTGTATAGTT865350.6916114054221503No Hit
TATTGCACTTGTCCCGGCCTGTA852910.6816690169279555No Hit
AGCAGCATTGTACAGGGCTATGA795720.6359612035852701No Hit
CAACGGAATCCCAAAAGCAGCTGT776800.6208398217275395No Hit
CAACGGAATCCCAAAAGCAGCT701560.5607059543398206No Hit
CATTGCACTTGTCTCGGTCTGA632140.5052235902508327No Hit
TGTAAACATCCCCGACTGGAAGCT547830.43784073060890577No Hit
CCCATAAAGTAGAAAGCACTAC546080.4364420827098028No Hit
ACTGGACTTGGAGTCAGAAGGC536330.4286496158433719No Hit
CACCCGGCTGTGTGCACATGTGC502260.4014199393162642No Hit
CCCATAAAGTAGAAAGCACT460200.3678044360955377No Hit
CTCCCACTGCTTCACTTGACTAG456730.36503111711845926No Hit
TTCTCACTACTGCACTTGACTAG440120.3517559504875447No Hit
TGTAAACATCCTACACTCTCAGCT399960.3196589792715586No Hit
TCGTACGACTCTTAGCGG395980.31647805433531295No Hit
TGAGGTAGTAGTTTGTACAGTT373070.2981677552676277No Hit
CTTCTCACTACTGCACTTGACTAG332640.26585499266149426No Hit
AACGGAATCCCAAAAGCAGCTG301660.241094928710517No Hit
CTACGCCTGTCTGAGCGTCGCT291970.23335041548634106No Hit
CCCCCACTGCTAAATTTGACTGGCTT288850.23085682608908314No Hit
ACCGGGTGCTGTAGGCTT284320.2272363260988337No Hit
TGATGTAGTAGGTTGTATAGTT271360.21687833937176254No Hit
TGAGGTAGTAGTTTGTGCTGTT269020.21500814732381912No Hit
TAGCAGCACGTAAATATTGGC268560.21464050273319774No Hit
TTAATGCTAATCGTGATAGGGGTT266890.21330579302376806No Hit
TATTGCACTTGTCCCGGCCTGTT249290.1992393912956467No Hit
AACATTCAACGCTGTCGGTGAGTT247660.1979366506810536No Hit
CAACACCAGTCGATGGGCTGT245060.19585865951667206No Hit
TAGCTTATCAGACTGATGTTGACA242110.19350093877246988No Hit
GCCTGTCTGAGCGTCGCT242000.1934130237616691No Hit
CGCCTGTCTGAGCGTCGCT235730.18840186814602589No Hit
CAAAGAATTCTCCTTTTGGGCT229090.18309499840314372No Hit
TACGCCTGTCTGAGCGTCGCT226760.18123279862890945No Hit
TTCACAGTGGCTAAGTTCCGC225040.17985812755093394No Hit
TATTGCACTTGTCCCGGCCTGTAG214360.1713223792295512No Hit
CACTAGATTGTGAGCTCCTGGA212280.16965998629804593No Hit
CAACGGAATCCCAAAAGCAGCTGA210650.1683572456834529No Hit
TTCACAGTGGCTAAGTTCTGC208740.16683072140500335No Hit
CTCCCACTGCTTCACTTGACTAGCCT207050.16548002714815532No Hit
ACCGGGTGCTGTAGGCTTA200000.15984547418319764No Hit
CCCCCCACTGCTAAATTTGACTGGCT199300.1592860150235564No Hit
TCGTTTCCCGGCCAATGCACCA196330.15691230973193593No Hit
TTCAAGTAATCCAGGATAGGCTT195350.15612906690843828No Hit
CCCCCCACTGCTAAATTTGACTGG184110.14714575125934257No Hit
ACCGGGTGCTGTAGGCTTT184090.14712976671192426No Hit
AGCTCGGTCTGAGGCCCCTCAGT183870.14695393669032275No Hit
TTCAAGTAATCCAGGATAGGC181930.14540343559074573No Hit
TAGCAGCACATCATGGTTTACA178940.14301374575170692No Hit
TGTCAGTTTGTCAAATACCCCA178030.14228644884417338No Hit
CACTAGATTGTGAGCTCCTGGAA172870.13816243561024685No Hit
TGAGGTAGTAGGTTGTGTGGTT172200.13762695327173313No Hit
AGCAGCATTGTACAGGGCTATGT169630.13557293892847905No Hit
GGAATACCGGGTGCTGTAGGCTTT166870.13336707138475096No Hit
TGAGAACTGAATTCCATGGGTT166670.13320722591056774No Hit
ATCACATTGCCAGGGATTTCC163930.13101734291425793No Hit
TGATGTAGTAGATTGTATAGTT163690.13082552834523808No Hit
TTCACAGTGGCTAAGTTCCG162610.12996236278464884No Hit
TGTAAACATCCTACACTCAGCT157920.12621398641505285No Hit
CAAAGTGCTGTTCGTGCAGGTAG157670.12601417957232383No Hit
TATTGCACTTGTCCCGGCCTGTTT155600.12435977891452775No Hit
TGTAAACATCCCCGACTGGAAGC153430.12262545551964005No Hit
GGAATACCGGGTGCTGTAGGCTT150200.12004395111158142No Hit
CCCATAAAGTAGAAAGCACTA148580.1187492027706975No Hit
ACCCCCCACTGCTAAATTTGACTG143300.1145292822522611No Hit
CAAAGAATTCTCCTTTTGGGCTT139230.11127642685263303No Hit
TATTGCACTTGTCCCGGCCTGA133960.10706449860790578No Hit
TTATAAAGCAATGAGACTGATT133220.10647307035342793No Hit
TGTCAGTTTGTCAAATACCCCAA133010.10630523260553558No Hit
AGCAGCATTGTACAGGGCTATG131980.10548202841349211No Hit
AATGCACCCGGGCAAGGATTCT131140.10481067742192267No Hit
TATTGCACTTGTCCCGGCCTGTAAT130930.10464283967403032No Hit
ACCACAGGGTAGAACCACGGAC130400.10421924916744485No Hit
CAAAGTGCTGTTCGTGCAGGT129770.10371573592376777No Hit
TGAGGTAGTAGGTTGTATAGT128680.10284457808946935No Hit
AATGACACGATCACTCCCGTTG128650.10282060126834186No Hit
TCCCACTGCTTCACTTGACTAG125160.10003129774384506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)