FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003947582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16593876
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG212532212.807869602014623No Hit
CCCCCCACTGCTAAATTTGACTG11052356.660499331199052No Hit
AACATTCAACGCTGTCGGTGAGT6357633.8313110210055803No Hit
TAGCAGCACGTAAATATTGGCG5625623.390178400754592No Hit
TTCAAGTAATCCAGGATAGGCT4815042.901696987491048No Hit
AAGCTGCCAGTTGAAGAACTGT4704372.835003708597075No Hit
AACATTCAACGCTGTCGGTGAG4530192.730037273991923No Hit
CCCCCACTGCTAAATTTGACTG3882222.339549843568796No Hit
TATTGCACTTGTCCCGGCCTGTAA3011941.8150913023575683No Hit
TAGCTTATCAGACTGATGTTGAC2581201.5555136123712145No Hit
TATTGCACTTGTCCCGGCCTGTAT2390871.4408146716294614No Hit
TAGCTTATCAGACTGATGTTGACT2384711.4371024587624976No Hit
TATTGCACTTGTCCCGGCCTGT1555810.9375808280114905No Hit
TAGCTTATCAGACTGATGTTGA1466140.8835428202548941No Hit
TGTAAACATCCTTGACTGGAAGCT1376360.8294385229828162No Hit
TGAGGTAGTAGGTTGTATAGTT1199280.7227244556967884No Hit
CAACGGAATCCCAAAAGCAGCTGT1178660.7102981847038028No Hit
TATTGCACTTGTCCCGGCCTGTA1112700.670548580693263No Hit
AGCAGCATTGTACAGGGCTATGA1100940.6634616288563323No Hit
ACTGGACTTGGAGTCAGAAGGC897880.5410911832774935No Hit
TGTAAACATCCCCGACTGGAAGCT881770.5313827824192491No Hit
CATAAAGTAGAAAGCACTACT828780.49944931491593647No Hit
CAACGGAATCCCAAAAGCAGCT784120.4725357716304497No Hit
TTCAAGTAATTCAGGATAGGTT720870.4344193002285904No Hit
CCCATAAAGTAGAAAGCACTAC664880.40067793684850966No Hit
CACCCGGCTGTGTGCACATGTGC609760.3674608632726917No Hit
CATTGCACTTGTCTCGGTCTGA602750.3632364132406437No Hit
TGAGGTAGTAGATTGTATAGTT578860.34883953574198096No Hit
CGCCTGTCTGAGCGTCGCT542180.32673499548869717No Hit
CCCATAAAGTAGAAAGCACT473970.28562946957058133No Hit
AACATTCAACGCTGTCGGTGAGTT462040.2784400703006338No Hit
CCCCCACTGCTAAATTTGACTGGCTT419450.25277397517011696No Hit
CTTCTCACTACTGCACTTGACTAG391660.2360268330316558No Hit
TTAATGCTAATCGTGATAGGGGTT387180.23332704185568218No Hit
TGTAAACATCCTACACTCTCAGCT386660.2330136732370424No Hit
TTCTCACTACTGCACTTGACTAG366420.2208164023884474No Hit
CAAAGAATTCTCCTTTTGGGCT365710.22038853369761227No Hit
TAGCTTATCAGACTGATGTTGACA365160.22005708612020483No Hit
AACGGAATCCCAAAAGCAGCTG360570.21729100542874974No Hit
CACTAGATTGTGAGCTCCTGGA348680.21012571143715914No Hit
TATTGCACTTGTCCCGGCCTGTAG342390.20633515641553546No Hit
TGATGTAGTAGGTTGTATAGTT338520.2040029707345047No Hit
TAGCAGCACGTAAATATTGGC328900.19820565128966855No Hit
CAACACCAGTCGATGGGCTGT325370.19607836047467153No Hit
CTACGCCTGTCTGAGCGTCGCT321070.193487043051304No Hit
CACTAGATTGTGAGCTCCTGGAA320230.19298083220580894No Hit
CTCCCACTGCTTCACTTGACTAG309270.1863759859360164No Hit
TCGTACGACTCTTAGCGG305010.1838087737910058No Hit
CAAAGAATTCTCCTTTTGGGCTT304030.18321819447126156No Hit
ACTTGACTTGGAGTCAGAAGGC303500.18289879953303256No Hit
CCCCCCACTGCTAAATTTGACTGG291520.1756792686651389No Hit
TATTGCACTTGTCCCGGCCTGTT285490.17204539795283513No Hit
TCGTTTCCCGGCCAATGCACCA278610.1678992900754471No Hit
TTCAAGTAATCCAGGATAGGCTT277400.16717010540515068No Hit
CCCCCCACTGCTAAATTTGACTGGCT274640.16550684119852407No Hit
CTCCCACTGCTTCACTTGACTAGCCT255500.15397246550474405No Hit
TGAGGTAGTAGTTTGTACAGTT252360.15208020115372684No Hit
CAACGGAATCCCAAAAGCAGCTGA246420.14850056731772612No Hit
TATTGCACTTGTCCCGGCCTGTAAT237570.14316727448126043No Hit
ACTGGACTTGGAGTCAGAAGGCT233950.14098574678996034No Hit
ACCGGGTGCTGTAGGCTT233010.14041927274857305No Hit
TGTCAGTTTGTCAAATACCCCAA232760.14026861475884236No Hit
ACCCCCCACTGCTAAATTTGACTG229940.1385691926346804No Hit
ACTGGACTTGGAGTCAGAAGGCA223990.13498353247909048No Hit
ACCACAGGGTAGAACCACGGAC223590.13474247969552142No Hit
TGTAAACATCCCCGACTGGAAGC215000.12956587116837562No Hit
TATTGCACTTGTCCCGGCCTGTAAA208450.12561863183743208No Hit
CAAAGTGCTGTTCGTGCAGGTAG207960.12532334217755994No Hit
TGAGGTAGTAGTTTGTGCTGTT206890.12467852598151269No Hit
CCTTGGCTTGGCTCTTGACGTGGACA202030.12174973466114848No Hit
AGCAGCATTGTACAGGGCTATGT200650.12091810255783518No Hit
TTCACAGTGGCTAAGTTCTGC195750.11796520595911408No Hit
TTCAAGTAATCCAGGATAGGC193980.11689854739182093No Hit
ATGCACCTGGGCAAGGATTCTGA191880.11563302027808331No Hit
AGCTCGGTCTGAGGCCCCTCAGT188150.11338520307130173No Hit
TACGCCTGTCTGAGCGTCGCT184680.11129407017384005No Hit
TGTCAGTTTGTCAAATACCCCA178480.10755775202851943No Hit
ACTGGACTTGGAGTCAGAAGG174300.10503875044022265No Hit
TGATGTAGTAGATTGTATAGTT166820.10053106338748102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)