FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9755946
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGGAATCCCAAAAGCAGCTG8963439.187658480274491No Hit
CCCCCCACTGCTAAATTTGACTG6428016.588812607203853No Hit
AACATTCAACGCTGTCGGTGAGT3174743.2541590533608935No Hit
TAGCAGCACGTAAATATTGGCG2950783.0245964871064275No Hit
TTCAAGTAATCCAGGATAGGCT2474392.5362891512519647No Hit
GGAATACCGGGTGCTGTAGGCTTT2346002.404687356818088No Hit
AACATTCAACGCTGTCGGTGAG2286752.3439551633434625No Hit
CCCCCACTGCTAAATTTGACTG2029492.080259566832371No Hit
TATTGCACTTGTCCCGGCCTGTAA2005992.056171692627245No Hit
AAGCTGCCAGTTGAAGAACTGT1953142.001999601063803No Hit
TATTGCACTTGTCCCGGCCTGTAT1937091.9855480954896636No Hit
GGAATACCGGGTGCTGTAGGCTT1651961.693285305187216No Hit
TAGCTTATCAGACTGATGTTGACT1317211.3501612247546266No Hit
TAGCTTATCAGACTGATGTTGAC1307161.3398598147222216No Hit
TATTGCACTTGTCCCGGCCTGT1068381.0951065124796715No Hit
TGAATACCGGGTGCTGTAGGCTTT964150.9882691027605114No Hit
TGTAAACATCCTTGACTGGAAGCT893960.9163232350814571No Hit
TATTGCACTTGTCCCGGCCTGTA849000.8702385191553952No Hit
TGAGGTAGTAGGTTGTATAGTT832560.8533872573710433No Hit
TAGCTTATCAGACTGATGTTGA759460.7784585933542478No Hit
TGAATACCGGGTGCTGTAGGCTT675970.6928800138910158No Hit
AGCAGCATTGTACAGGGCTATGA675470.6923675059292046No Hit
TGTAAACATCCCCGACTGGAAGCT570860.585140590159068No Hit
ACTGGACTTGGAGTCAGAAGGC539550.5530473415904515No Hit
CATAAAGTAGAAAGCACTACT536990.5504233008259783No Hit
TGAGGTAGTAGATTGTATAGTT521280.5343203006658709No Hit
CAACGGAATCCCAAAAGCAGCTGT494090.5064501177025785No Hit
CCCATAAAGTAGAAAGCACTAC481040.4930736598993065No Hit
TTCAAGTAATTCAGGATAGGTT457920.4693752917451573No Hit
GGAATACCGGGTGCTGTAGGCTTA419480.42997367964111327No Hit
CATTGCACTTGTCTCGGTCTGA371450.38074216482953066No Hit
GCCTGTCTGAGCGTCGCT316180.32408953473092206No Hit
TTAATGCTAATCGTGATAGGGGTT291200.29848463695883515No Hit
TCGTACGACTCTTAGCGG289000.2962296019268659No Hit
CACCCGGCTGTGTGCACATGTGC282940.29001800542971434No Hit
TTCTCACTACTGCACTTGACTAG261110.2676419078170379No Hit
TATTGCACTTGTCCCGGCCTGTAG260390.2669038963520298No Hit
CCCATAAAGTAGAAAGCACT259230.26571487788062786No Hit
GGAATACCGGGTGCTGTAGGCT257280.26371609682956426No Hit
ACCGGGTGCTGTAGGCTTA250910.2571867453960897No Hit
CGCCTGTCTGAGCGTCGCT249530.25577222342149086No Hit
AACATTCAACGCTGTCGGTGAGTT246440.25260492421749775No Hit
ACCGGGTGCTGTAGGCTT243950.250052634567678No Hit
CACTAGATTGTGAGCTCCTGGAA243260.24934537358037856No Hit
CTTCTCACTACTGCACTTGACTAG237740.24368728568198308No Hit
CTCCCACTGCTTCACTTGACTAG234960.24083774141431288No Hit
CCCCCACTGCTAAATTTGACTGGCTT234720.2405917375926435No Hit
CACTAGATTGTGAGCTCCTGGA226090.2317458501717824No Hit
TGTAAACATCCTACACTCTCAGCT225140.23077208504434116No Hit
CAAAGAATTCTCCTTTTGGGCT222910.22848629953466326No Hit
TATTGCACTTGTCCCGGCCTGTT216790.22221320208209433No Hit
TGAGGTAGTAGTTTGTACAGTT214340.21970191306921955No Hit
TGTCAGTTTGTCAAATACCCCAA197370.20230739284534785No Hit
ATCCCGGACGAGCCCCCA196890.20181538520200912No Hit
AGCTCGGTCTGAGGCCCCTCAGT194550.19941684794073278No Hit
TAGCAGCACGTAAATATTGGC190020.1947735258067234No Hit
TAGCTTATCAGACTGATGTTGACA188280.19298999809962047No Hit
ACCGGGTGCTGTAGGCTTT186970.19164722723967517No Hit
CAACACCAGTCGATGGGCTGT186080.19073496306765125No Hit
AACGGAATCCCAAAAGCAGCTG177510.1819505766022075No Hit
TGAATACCGGGTGCTGTAGGCTTA171990.176292488703812No Hit
TATTGCACTTGTCCCGGCCTGTTT165780.16992713981811708No Hit
CCCCCCACTGCTAAATTTGACTGGCT165530.1696708858372115No Hit
TATTGCACTTGTCCCGGCCTGTAAT161810.16585782660133624No Hit
TGATGTAGTAGGTTGTATAGTT160010.16401279793881599No Hit
CTCCCACTGCTTCACTTGACTAGCCT158760.162731528034288No Hit
ACTGGACTTGGAGTCAGAAGGCA158250.1622087699132406No Hit
TATTGCACTTGTCCCGGCCTGTAAA157750.16169626195142942No Hit
CAAAGAATTCTCCTTTTGGGCTT153190.1570221893397114No Hit
CCCCCCACTGCTAAATTTGACTGG153160.15699143886200273No Hit
ACCCCCCACTGCTAAATTTGACTG150120.15387539045419069No Hit
TGAGGTAGTAGTTTGTGCTGTT146950.15062608997630778No Hit
TGTAAACATCCCCGACTGGAAGC142090.14564451258750305No Hit
TCGTTTCCCGGGCGGCGCACCA141160.14469124777853423No Hit
TTCAAGTAATCCAGGATAGGCTT141070.14459899634540824No Hit
ACTGGACTTGGAGTCAGAAGGCT140760.1442812414090853No Hit
CTACGCCTGTCTGAGCGTCGCT138240.1416982012815569No Hit
CAACGGAATCCCAAAAGCAGCT136250.13965841959354838No Hit
TGTCAGTTTGTCAAATACCCCA135830.139227912905627No Hit
GAATACCGGGTGCTGTAGGCTTT135010.13838739984825665No Hit
AGCAGCATTGTACAGGGCTATGT132160.13546610446593288No Hit
TCCTGTCTGAGCGTCGCT128200.13140704140838827No Hit
TGAGAACTGAATTCCATGGGTT123180.12626146147180398No Hit
ACTGGACTTGGAGTCAGAAGG116450.11936310430582539No Hit
TTCAAGTAATCCAGGATAGGC113760.11660581147128121No Hit
CAAAGTGCTGTTCGTGCAGGTAG112730.11555004506995016No Hit
AACCGGGCGGAAACACCA112110.11491453519730428No Hit
ATCCCACCGCTGCCACCA110970.1137460170443748No Hit
ACTTGACTTGGAGTCAGAAGGC109460.112198242999705No Hit
AGCTACATCTGGCTACTGGGTCTC107060.1097382047830113No Hit
TGAATACCGGGTGCTGTAGGCT104480.10709366370006558No Hit
ACCACAGGGTAGAACCACGGAC104350.10696041162999467No Hit
TGATGTAGTAGATTGTATAGTT102190.10474637723497035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)