FastQCFastQC Report
Sat 10 Sep 2022
EGAF00003947585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6548858
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG5660618.64365970372239No Hit
CAACGGAATCCCAAAAGCAGCTG4149976.336936913275566No Hit
GCCTGTCTGAGCGTCGCT3372345.149508509727956No Hit
CCCCCACTGCTAAATTTGACTG2318383.5401286758698998No Hit
AACATTCAACGCTGTCGGTGAGT1953872.9835278150785984No Hit
TAGCAGCACGTAAATATTGGCG1550632.367786872153893No Hit
TTCAAGTAATCCAGGATAGGCT1506072.2997444745328117No Hit
TAGCTTATCAGACTGATGTTGAC1449962.214065414153124No Hit
TCCTGTCTGAGCGTCGCT1395062.1302340041576713No Hit
AACATTCAACGCTGTCGGTGAG1279981.9545087097628318No Hit
TAGCTTATCAGACTGATGTTGACT1269791.9389487449567544No Hit
AAGCTGCCAGTTGAAGAACTGT1162881.7756989081149719No Hit
TATTGCACTTGTCCCGGCCTGTAA1004821.534343850485077No Hit
TGAGGTAGTAGGTTGTATAGTT973811.4869920831998495No Hit
TAGCTTATCAGACTGATGTTGA932431.423805494026592No Hit
TATTGCACTTGTCCCGGCCTGTAT825821.2610137523213971No Hit
TGAGGTAGTAGATTGTATAGTT719591.0988022644558793No Hit
TATTGCACTTGTCCCGGCCTGT512620.7827624297243886No Hit
TGTAAACATCCTTGACTGGAAGCT505860.7724400193132909No Hit
CATAAAGTAGAAAGCACTACT498930.7618580216581272No Hit
GGAATACCGGGTGCTGTAGGCTTT488770.7463438663657084No Hit
TTCAAGTAATTCAGGATAGGTT473830.7235307285636672No Hit
TATTGCACTTGTCCCGGCCTGTA399480.6099994838794794No Hit
TGTAAACATCCCCGACTGGAAGCT388930.5938898049094972No Hit
AGCAGCATTGTACAGGGCTATGA387320.5914313610098128No Hit
ACTGGACTTGGAGTCAGAAGGC308100.4704637052750266No Hit
GGAATACCGGGTGCTGTAGGCTT304480.46493602396020806No Hit
CATTGCACTTGTCTCGGTCTGA285520.43598441132789867No Hit
TGAATACCGGGTGCTGTAGGCTTT244720.3736834727520432No Hit
TGAGGTAGTAGTTTGTACAGTT236810.3616050309840281No Hit
TTCTCACTACTGCACTTGACTAG234760.35847471421734906No Hit
CAACGGAATCCCAAAAGCAGCTGT232320.3547488737731067No Hit
CAACGGAATCCCAAAAGCAGCT221470.3381810996665373No Hit
TGATGTAGTAGGTTGTATAGTT215220.32863745098763786No Hit
CAACACCAGTCGATGGGCTGT213290.3256903722755937No Hit
CACCCGGCTGTGTGCACATGTGC212930.3251406581116891No Hit
TAGCTTATCAGACTGATGTTGACA200930.3068168526482022No Hit
TGTCAGTTTGTCAAATACCCCAA200850.30669469394511223No Hit
CCCATAAAGTAGAAAGCACTAC174790.2669014964135732No Hit
CTTCTCACTACTGCACTTGACTAG174570.2665655599800759No Hit
TCGTACGACTCTTAGCGG173260.2645652112169786No Hit
CCCCCACTGCTAAATTTGACTGGCTT166530.2542886103195397No Hit
TGATGTAGTAGATTGTATAGTT158860.24257664466079432No Hit
TGAATACCGGGTGCTGTAGGCTT155350.2372169315627244No Hit
CCCTGTCTGAGCGTCGCT150370.2296125522953773No Hit
TTATAAAGCAATGAGACTGATT147070.2245735057929184No Hit
AACATTCAACGCTGTCGGTGAGTT143410.21898474512655489No Hit
TGAGGTAGTAGTTTGTGCTGTT141310.2157780791704447No Hit
CACTAGATTGTGAGCTCCTGGA136630.2086317950396848No Hit
CAAAGAATTCTCCTTTTGGGCT135500.20690630335853977No Hit
CCCATAAAGTAGAAAGCACT135380.20672306530390488No Hit
CACTAGATTGTGAGCTCCTGGAA121120.18494827647812795No Hit
TATTGCACTTGTCCCGGCCTGTAG117380.17923735710867453No Hit
TGTCAGTTTGTCAAATACCCCA116740.17826008748395522No Hit
TAGCAGCACGTAAATATTGGC114170.17433573914719178No Hit
CCCCCCACTGCTAAATTTGACTGG109980.1679376770728576No Hit
TGTAAACATCCTACACTCTCAGCT109310.16691459793447957No Hit
CAAAGAATTCTCCTTTTGGGCTT108520.16570828074146668No Hit
ACCCCCCACTGCTAAATTTGACTG102980.15724879055249022No Hit
TATTGCACTTGTCCCGGCCTGTAAT97760.14927793517587343No Hit
TCGTTTCCCGGCCAATGCACCA97580.1490030780939211No Hit
CCCCCCACTGCTAAATTTGACTGGCT95870.14639193581537424No Hit
TATTGCACTTGTCCCGGCCTGTT93370.14257447634381445No Hit
TTCAAGTAATCCAGGATAGGCTT92670.1415055876917777No Hit
TGTAAACATCCCCGACTGGAAGC91940.14039088952608225No Hit
AACGGAATCCCAAAAGCAGCTG90340.137947715464284No Hit
CTCCCACTGCTTCACTTGACTAG89420.13654289037875No Hit
TTCACAGTGGCTAAGTTCCGC87390.13344311328784347No Hit
CGCCTGTCTGAGCGTCGCT87340.13336676409841228No Hit
ACCGGGTGCTGTAGGCTT85400.1304044155484819No Hit
ACTGGACTTGGAGTCAGAAGGCT84310.12874000321888182No Hit
ACTGGACTTGGAGTCAGAAGGCA84100.12841933662327082No Hit
CTCCCACTGCTTCACTTGACTAGCCT79570.1215021000608045No Hit
TGAGGTAGTAGGTTGTATAGT78440.11977660837965948No Hit
AGCAGCATTGTACAGGGCTATGT76240.11641724404468688No Hit
CCCGCCACTGCTAAATTTGACTG76000.11605076793541713No Hit
ACTTGACTTGGAGTCAGAAGGC75940.1159591489080997No Hit
TGAGGTAGTAAGTTGTATTGTT73950.11292045116873813No Hit
TTCACAGTGGCTAAGTTCTGC73870.11279829246564821No Hit
GGAATACCGGGTGCTGTAGGCTTA72230.110294039052305No Hit
TATTGCACTTGTCCCGGCCTGTAAA71370.10898083299408844No Hit
CGCGACCTCAGATCAGACGTGGCG70830.10815626174823152No Hit
CAACGGAATCCCAAAAGCAGCTGA69390.10595740509261309No Hit
ATGCACCTGGGCAAGGATTCTGA68850.10513283384675617No Hit
ACCGGGTGCTGTAGGCTTT68270.10424718324935432No Hit
TTCAAGTAATCCAGGATAGGC66810.1020177869179634No Hit
TGAGGTAGTAGATTGTATAGT66410.10140699340251386No Hit
ACCGGGTGCTGTAGGCTTA65980.10075039037340555No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)