FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7686389
Sequences flagged as poor quality0
Sequence length18-26
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG6404698.332508281847302No Hit
CAACGGAATCCCAAAAGCAGCTG4609365.996782104054323No Hit
TAGCAGCACGTAAATATTGGCG2900463.773501445216993No Hit
AACATTCAACGCTGTCGGTGAGT2337193.0406865954871654No Hit
AAGCTGCCAGTTGAAGAACTGT2308883.0038552563498935No Hit
CCCCCACTGCTAAATTTGACTG2190892.8503501449119995No Hit
TTCAAGTAATCCAGGATAGGCT2147232.793548439976171No Hit
TAGCTTATCAGACTGATGTTGAC1714092.230032854178991No Hit
GCCTGTCTGAGCGTCGCT1622822.111290490241907No Hit
TAGCTTATCAGACTGATGTTGACT1351951.758888341456567No Hit
TAGCTTATCAGACTGATGTTGA1114441.4498875870060701No Hit
AACATTCAACGCTGTCGGTGAG1070011.3920841112777405No Hit
TCCTGTCTGAGCGTCGCT936151.217932113506095No Hit
TATTGCACTTGTCCCGGCCTGTAA813731.0586635675087483No Hit
CATAAAGTAGAAAGCACTACT737940.9600606995040194No Hit
TATTGCACTTGTCCCGGCCTGTAT715220.9305019561200975No Hit
TATTGCACTTGTCCCGGCCTGT633600.8243142521150049No Hit
TGTAAACATCCTTGACTGGAAGCT632260.8225709107358474No Hit
TGAGGTAGTAGGTTGTATAGTT449680.5850341428205104No Hit
TTCAAGTAATTCAGGATAGGTT430460.5600289030388652No Hit
AGCAGCATTGTACAGGGCTATGA418250.5441436804720656No Hit
TGTAAACATCCCCGACTGGAAGCT394250.5129196557707396No Hit
TATTGCACTTGTCCCGGCCTGTA366240.4764786169422339No Hit
ACTGGACTTGGAGTCAGAAGGC347280.4518116374281863No Hit
CCCATAAAGTAGAAAGCACTAC341460.4442398114381148No Hit
CAACGGAATCCCAAAAGCAGCTGT308050.40077336705181066No Hit
TGAGGTAGTAGATTGTATAGTT257800.3353980653334095No Hit
CTTCTCACTACTGCACTTGACTAG256700.33396696420126537No Hit
CATTGCACTTGTCTCGGTCTGA246040.3200982932297598No Hit
TGTAAACATCCTACACTCTCAGCT245700.31965595287982435No Hit
TTCTCACTACTGCACTTGACTAG244170.3176654213051148No Hit
AACATTCAACGCTGTCGGTGAGTT232260.30217049904708176No Hit
TAGCTTATCAGACTGATGTTGACA201250.26182645713091024No Hit
CCCATAAAGTAGAAAGCACT200440.26077264629724045No Hit
TTAATGCTAATCGTGATAGGGGTT199660.2597578654944474No Hit
CAAAGAATTCTCCTTTTGGGCT199410.2594326152371419No Hit
CCCCCACTGCTAAATTTGACTGGCTT196120.25515232185100184No Hit
CGCCTGTCTGAGCGTCGCT191250.2488164468386911No Hit
TCGTACGACTCTTAGCGG186510.2426497019601792No Hit
TTTAAACATCCTTGACTGGAAGCT185270.24103646068394405No Hit
CAAAGAATTCTCCTTTTGGGCTT174050.22643922913607417No Hit
CGCCCCACTGCTAAATTTGACTG162790.2117899575470354No Hit
GGAATACCGGGTGCTGTAGGCTTT162210.21103537695008673No Hit
ACTTGACTTGGAGTCAGAAGGC158720.20649488335810223No Hit
CCCCCCACTGCTAAATTTGACTGGCT153680.1999378381708238No Hit
CCCCCCACTGCTAAATTTGACTGG152630.19857178709014076No Hit
CACCCGGCTGTGTGCACATGTGC144200.18760434841380003No Hit
CAACGGAATCCCAAAAGCAGCT141830.18452097597454412No Hit
TGATGTAGTAGGTTGTATAGTT141630.1842607757686997No Hit
TTCAAGTAATCCAGGATAGGCTT140020.18216616411165243No Hit
TGTCAGTTTGTCAAATACCCCAA137080.17834122108574No Hit
TAGCAGCACGTAAATATTGGC134680.1752188186156074No Hit
CACTAGATTGTGAGCTCCTGGA131620.17123775546618836No Hit
TTATAAAGCAATGAGACTGATT127350.16568248107141076No Hit
CAACACCAGTCGATGGGCTGT125430.1631845590953047No Hit
CCCGCCACTGCTAAATTTGACTG119850.15592497335224642No Hit
TGTAAACATCCCCGACTGGAAGC119640.15565176313610982No Hit
TTTAAACATCCCCGACTGGAAGCT117240.15252936066597722No Hit
ATCAGCATTGTACAGGGCTATGA116840.15200896025428845No Hit
TATTGCACTTGTCCCGGCCTGTT116750.15189187016165848No Hit
CTCCCACTGCTTCACTTGACTAG115830.15069494921477433No Hit
GGAATACCGGGTGCTGTAGGCTT114690.14921180804146136No Hit
CTACGCCTGTCTGAGCGTCGCT113170.14723428647704404No Hit
TGTAAACATCCTACACTCAGCT113140.14719525644616738No Hit
TAGCAGCACGTAAATATTGGCGT112370.1461934856536665No Hit
ACCCCCCACTGCTAAATTTGACTG109560.1425376727615529No Hit
CACTAGATTGTGAGCTCCTGGAA108680.14139279185583764No Hit
TTCACAGTGGCTAAGTTCCG107360.13967547049726473No Hit
AACCGGGCGGAAACACCA102700.1336128057010906No Hit
TGAGGTAGTAGTTTGTACAGTT101920.1325980248982975No Hit
TGTCAGTTTGTCAAATACCCCA101020.13142712397199777No Hit
CCCTCCACTGCTAAATTTGACTG97700.127107800554981No Hit
TGAATACCGGGTGCTGTAGGCTTT97270.1265483701124156No Hit
TTCAAGTAATCCAGGATAGGC92870.12082396558383916No Hit
TATTGCACTTGTCCCGGCCTGTAG91570.11913266424585069No Hit
ACCGGGTGCTGTAGGCTT89070.1158801616727959No Hit
ACTGGACTTGGAGTCAGAAGGCA88090.11460518066415842No Hit
ACCACAGGGTAGAACCACGGAC87740.11414983030393075No Hit
TGCAAGTAATCCAGGATAGGCT86870.1130179594085077No Hit
CAGTGCAATAGTATTGTCAAAGC85340.11102742783379817No Hit
TGAGGTAGTAGTTTGTGCTGTT84460.10988254692808287No Hit
AGCAGCATTGTACAGGGCTATGT84140.10946622659873187No Hit
CTCCCACTGCTTCACTTGACTAGCCT83030.10802211545629554No Hit
TGATGTAGTAGATTGTATAGTT81550.10609663393304711No Hit
CCCATAAAGTAGAAAGCACTA80830.10515991319200732No Hit
TCGTTTCCCGGCCAATGCACCA79770.10378085210103209No Hit
ATCACATTGCCAGGGATTTCCA79270.10313035158642114No Hit
TTAGGTAGTAGGTTGTATAGTT78430.10203751072187474No Hit
CAAAGTGCTGTTCGTGCAGGTAG77700.10108777997054275No Hit
TTCACAGTGGCTAAGTTCCGC77550.10089262981615944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)