FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003947587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5221729
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG2651335.077494446762749No Hit
CAACGGAATCCCAAAAGCAGCTG2114414.049252651755769No Hit
TTCAAGTAATCCAGGATAGGCT1727493.308272030203023No Hit
TAGCAGCACGTAAATATTGGCG1622873.1079169370911437No Hit
AAGCTGCCAGTTGAAGAACTGT1589693.044374765523067No Hit
AACATTCAACGCTGTCGGTGAGT1346942.579490433149633No Hit
CCCCCACTGCTAAATTTGACTG1211942.320955377040823No Hit
CATAAAGTAGAAAGCACTACT1151502.205208274883664No Hit
TAGCTTATCAGACTGATGTTGAC969471.8566072655245034No Hit
GCCTGTCTGAGCGTCGCT830221.5899331428344903No Hit
TAGCTTATCAGACTGATGTTGA706591.3531724836735113No Hit
AACATTCAACGCTGTCGGTGAG656561.2573613069540759No Hit
TAGCTTATCAGACTGATGTTGACT630571.2075885209669057No Hit
TGAGGTAGTAGGTTGTATAGTT543411.0406706284450995No Hit
CGCCTGTCTGAGCGTCGCT521850.9993816224472775No Hit
TGTAAACATCCTTGACTGGAAGCT428940.8214520516097255No Hit
TATTGCACTTGTCCCGGCCTGT406610.7786884382548386No Hit
TGAGGTAGTAGATTGTATAGTT322820.6182243467633038No Hit
TATTGCACTTGTCCCGGCCTGTAA292990.5610976747357054No Hit
TTCAAGTAATTCAGGATAGGTT267040.511401491728123No Hit
TCCTGTCTGAGCGTCGCT265030.5075521920038363No Hit
ACCGGGTGCTGTAGGCTT264430.5064031473100193No Hit
CCCATAAAGTAGAAAGCACTAC254520.48742475911714306No Hit
TATTGCACTTGTCCCGGCCTGTAT251510.48166038490316143No Hit
AGCAGCATTGTACAGGGCTATGA249740.47827070305640146No Hit
TTCTCACTACTGCACTTGACTAG212940.4077959618356296No Hit
TATTGCACTTGTCCCGGCCTGTA210270.40268271294814423No Hit
CTACGCCTGTCTGAGCGTCGCT199570.3821914159084089No Hit
TGTAAACATCCCCGACTGGAAGCT195370.37414810305169033No Hit
TTCACAGTGGCTAAGTTCCG185850.355916593909795No Hit
ACTGGACTTGGAGTCAGAAGGC183550.3515119225834968No Hit
TGTAAACATCCTACACTCTCAGCT173470.3322079717273723No Hit
TACGCCTGTCTGAGCGTCGCT172500.33035034947236824No Hit
CATTGCACTTGTCTCGGTCTGA169050.32374334248292086No Hit
ACCGGGTGCTGTAGGCTTT168670.32301561417683683No Hit
CCCATAAAGTAGAAAGCACT164180.3144169297181068No Hit
ACGCCTGTCTGAGCGTCGCT159710.3058565467491706No Hit
TGAGGTAGTAGTTTGTACAGTT149270.28586316907675596No Hit
CAACGGAATCCCAAAAGCAGCTGT145960.2795242725158659No Hit
AACATTCAACGCTGTCGGTGAGTT145160.27799221292411No Hit
TGTCAGTTTGTCAAATACCCCAA144550.2768240174853961No Hit
TTAATGCTAATCGTGATAGGGGTT139630.26740185099609726No Hit
CAACACCAGTCGATGGGCTGT137530.263380194567738No Hit
TAGCAGCACATCATGGTTTACA135630.2597415530373177No Hit
CTTCTCACTACTGCACTTGACTAG127870.24488057499728538No Hit
TTCACAGTGGCTAAGTTCCGC124260.23796715608948682No Hit
ACCGGGTGCTGTAGGCTTA123490.2364925487324218No Hit
TGTAAACATCCTACACTCAGCT114360.21900791864150745No Hit
TGTCAGTTTGTCAAATACCCCA110430.21148167589700653No Hit
TTATAAAGCAATGAGACTGATT110180.21100290727458282No Hit
TGATGTAGTAGGTTGTATAGTT107220.20533428678508595No Hit
TCGTACGACTCTTAGCGG103750.19868897830584464No Hit
GCGTTCGCGCTTTCCCCTG100360.192196875785779No Hit
GGAATACCGGGTGCTGTAGGCTTT98260.18817521935741974No Hit
CAACGGAATCCCAAAAGCAGCT96340.18449827633720556No Hit
TAGCAGCACATAATGGTTTGT95460.18281301078627404No Hit
CCCCCACTGCTAAATTTGACTGGCTT92500.1771443902967772No Hit
TGAGGTAGTAGTTTGTGCTGTT91200.17465479346017382No Hit
CCCCCCACTGCTAAATTTGACTGG90330.17298867865413928No Hit
TAGCTTATCAGACTGATGTTGACA89380.17116935788892915No Hit
CTCCCACTGCTTCACTTGACTAG84690.16218765853226008No Hit
TAGCAGCACGTAAATATTGGC83450.15981296616503846No Hit
ATCACATTGCCAGGGATTTCC82080.15718931411415643No Hit
TTCAAGTAATCCAGGATAGGC79990.15318680843069413No Hit
TCGTTTCCCGGCCAATGCACCA78450.150237593716564No Hit
CAAAGAATTCTCCTTTTGGGCT75830.14522009855356338No Hit
GGAATACCGGGTGCTGTAGGCTT75730.1450285911045939No Hit
TAGCAGCACGTAAATATTGGCGT74770.14319011959448683No Hit
TCCACCACGTTCCCGTGG71890.13767470506416554No Hit
TTCAAGTAATCCAGGATAGGCTT71110.1361809469622035No Hit
TTCACAGTGGCTAAGTTCTGC70730.13545321865611945No Hit
TCAGGCTCAGTCCCCTCCCGAT69350.1328104158603405No Hit
CATAAAGTAGAAAGCACTAC68330.13085703988085173No Hit
TGTCAGTTTGTCAAATACC64930.1243457866158891No Hit
TATTGCACTTGTCCCGGCCTGTT64000.12256476734047285No Hit
TGATGTAGTAGATTGTATAGTT63950.12246901361598812No Hit
CCCCCCACTGCTAAATTTGACTGGCT63330.12128166743237728No Hit
ATCCCGGACGAGCCCCCA61560.11789198558561734No Hit
CACCCGGCTGTGTGCACATGTGC61530.11783453335092649No Hit
TGAGGTAGTAGGTTGTGTGGTT59870.11465550969803297No Hit
TCAGGCTCAGTCCCCTCCCGA59100.1131809023409679No Hit
ACTGGACTTGGAGTCAGAAGG58500.11203185764715098No Hit
ACCACAGGGTAGAACCACGGAC58210.11147648604513945No Hit
CAAAGTGCTGTTCGTGCAGGT57850.1107870592288493No Hit
AGCAGCATTGTACAGGGCTATG57590.11028913986152863No Hit
AACCGGGCGGAAACACCA56580.10835491462693678No Hit
TTCACAGTGGCTAAGTTCTG56410.10802935196368867No Hit
TACCTGGTTGATCCTGCC55900.10705266397394426No Hit
TGAGGTAGTAGGTTGTATAGT55640.10655474460662358No Hit
ACCTGGTGCTGTAGGCTT54840.10502268501486767No Hit
TGCAAGTAATCCAGGATAGGCT54730.10481202682100124No Hit
GTCAGGATGGCCGAGCGGTCTAAGG54650.10465882086182565No Hit
TGTAAACATCCCCGACTGGAAGC54280.10395024330063854No Hit
CAAAGAATTCTCCTTTTGGGCTT53790.10301185680068804No Hit
ATCCCACCGCTGCCACCA53450.10236073147419178No Hit
ATCACATTGCCAGGGATTTCCA52370.1002924510253213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)