FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7279798
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG4641426.375753832729974No Hit
CAACGGAATCCCAAAAGCAGCTG4622446.349681680727954No Hit
TAGCAGCACGTAAATATTGGCG2564143.5222680629325156No Hit
TTCAAGTAATCCAGGATAGGCT2345613.222081162142136No Hit
AAGCTGCCAGTTGAAGAACTGT2310613.1740029050256613No Hit
CCCCCACTGCTAAATTTGACTG1983982.725322872969827No Hit
AACATTCAACGCTGTCGGTGAGT1966072.7007205419710822No Hit
AACATTCAACGCTGTCGGTGAG990531.360655886330912No Hit
TAGCTTATCAGACTGATGTTGAC980161.3464109855795448No Hit
CATAAAGTAGAAAGCACTACT907281.2462983176181537No Hit
CGCCTGTCTGAGCGTCGCT861591.1835355871138182No Hit
GCCTGTCTGAGCGTCGCT834961.146954901770626No Hit
TAGCTTATCAGACTGATGTTGA820571.1271878697733095No Hit
TGAGGTAGTAGGTTGTATAGTT656560.9018931569255082No Hit
TAGCTTATCAGACTGATGTTGACT653880.8982117360948751No Hit
TATTGCACTTGTCCCGGCCTGT606000.8324406803595373No Hit
TGTAAACATCCTTGACTGGAAGCT514620.7069152193508667No Hit
TATTGCACTTGTCCCGGCCTGTAA458630.6300037446093971No Hit
TATTGCACTTGTCCCGGCCTGTAT395450.5432156221917147No Hit
AGCAGCATTGTACAGGGCTATGA395000.5425974731716456No Hit
TGAGGTAGTAGATTGTATAGTT394310.5416496446742066No Hit
TTCAAGTAATTCAGGATAGGTT394280.5416084347395353No Hit
TTCTCACTACTGCACTTGACTAG383730.5271162743801407No Hit
CCCATAAAGTAGAAAGCACTAC327840.4503421660875755No Hit
TATTGCACTTGTCCCGGCCTGTA291200.40001109920907146No Hit
TGTAAACATCCCCGACTGGAAGCT279530.3839804346219497No Hit
CAACGGAATCCCAAAAGCAGCTGT275890.3789802958818363No Hit
TCCTGTCTGAGCGTCGCT275420.37833467357198647No Hit
ACTGGACTTGGAGTCAGAAGGC266630.366260162713306No Hit
CTACGCCTGTCTGAGCGTCGCT255540.3510262235298287No Hit
ACCGGGTGCTGTAGGCTT236610.325022754752261No Hit
CTTCTCACTACTGCACTTGACTAG227240.3121515184899361No Hit
ACGCCTGTCTGAGCGTCGCT221570.3043628408370672No Hit
CATTGCACTTGTCTCGGTCTGA218720.30044789704329705No Hit
CCCATAAAGTAGAAAGCACT208690.2866700422182044No Hit
TACGCCTGTCTGAGCGTCGCT202530.2782082689657048No Hit
CTCCCACTGCTTCACTTGACTAG186990.25686152280598995No Hit
TTCACAGTGGCTAAGTTCCG185300.25454002981950874No Hit
CCCCCACTGCTAAATTTGACTGGCTT183210.25166907103741065No Hit
TGATGTAGTAGGTTGTATAGTT180060.2473420278969279No Hit
TGTAAACATCCTACACTCTCAGCT177340.24360566048673327No Hit
AACATTCAACGCTGTCGGTGAGTT176330.24221825935280072No Hit
TGAGGTAGTAGTTTGTACAGTT169460.2327811843130812No Hit
CAACACCAGTCGATGGGCTGT155460.21354988146649123No Hit
ACCGGGTGCTGTAGGCTTT144220.19810989260965758No Hit
CCCCCCACTGCTAAATTTGACTGG144020.19783515971184915No Hit
CCCCCCACTGCTAAATTTGACTGGCT142420.19563729652938172No Hit
TAGCAGCACGTAAATATTGGC136170.18705189347286835No Hit
CACCCGGCTGTGTGCACATGTGC125520.17242236666456953No Hit
TGTAAACATCCTACACTCAGCT124760.1713783816528975No Hit
GCGTTCGCGCTTTCCCCTG124350.17081517921239023No Hit
TGAGGTAGTAGTTTGTGCTGTT121610.16705133851241477No Hit
ACCGGGTGCTGTAGGCTTA121160.1664331894923458No Hit
TTCAAGTAATCCAGGATAGGC117850.16188636003361634No Hit
TGTCAGTTTGTCAAATACCCCA113710.15619938904898187No Hit
AGCTCGGTCTGAGGCCCCTCAGT111030.15251796821834893No Hit
CAACGGAATCCCAAAAGCAGCT110450.15172124281470448No Hit
TAGCAGCACGTAAATATTGGCGT107670.14790245553516734No Hit
TGATGTAGTAGATTGTATAGTT106970.14694089039283784No Hit
TAGCTTATCAGACTGATGTTGACA106530.14633647801765928No Hit
TTCAAGTAATCCAGGATAGGCTT106020.14563590912824778No Hit
TCGTACGACTCTTAGCGG104070.14295726337461562No Hit
TGTCAGTTTGTCAAATACCCCAA102800.1412127094735321No Hit
TTATAAAGCAATGAGACTGATT100790.1384516438505574No Hit
TACCTGGTTGATCCTGCC98830.1357592614520348No Hit
CAAAGTGCTGTTCGTGCAGGT97730.13424823051408843No Hit
AACGGAATCCCAAAAGCAGCTG96670.13279214615570378No Hit
TGTAAACATCCCCGACTGGAAGC96180.13211905055607312No Hit
TATTGCACTTGTCCCGGCCTGTT93450.1283689465009881No Hit
TTCACAGTGGCTAAGTTCCGC92900.12761343103201492No Hit
CAAAGAATTCTCCTTTTGGGCT91960.1263221864123153No Hit
AGCAGCATTGTACAGGGCTATG91330.12545677778421874No Hit
ACCCCCCACTGCTAAATTTGACTG89740.12327265124664173No Hit
CTCCCACTGCTTCACTTGACTAGCCT89600.12308033821817584No Hit
TAGTGGGTTATCAGAACT86890.11935770745287162No Hit
AACCGGGCGGAAACACCA86760.11917913106929616No Hit
ACCACAGGGTAGAACCACGGAC85270.11713237098062337No Hit
TAGCAGCACATAATGGTTTGT84980.11673400827880115No Hit
GGGCTACGCCTGTCTGAGCGTCGCT83100.11415151903940192No Hit
TGAGGTAGTAGGTTGTGTGGTT82270.11301137751349694No Hit
TTCACAGTGGCTAAGTTCTGC82180.11288774770948315No Hit
ACTTGACTTGGAGTCAGAAGGC79640.10939863990731612No Hit
GTCAGGATGGCCGAGCGGTCTAAGG78770.10820355180184944No Hit
GCTTGACTAGCTTGCTGTTT78010.10715956679017741No Hit
AGCAGCATTGTACAGGGCTATGT76030.10443971110187399No Hit
TCCCACTGCTTCACTTGACTAG74170.1018846951522556No Hit
ATCCCACCGCTGCCACCA74140.10184348521758432No Hit
CAAAGAATTCTCCTTTTGGGCTT73500.10096433994459736No Hit
TGAGGTAGTAGGTTGTATAGT73250.10062092382233682No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)