FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9007069
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG206536622.930500476903195No Hit
CAACGGAATCCCAAAAGCAGCTG6984457.754409342262172No Hit
CCCCCACTGCTAAATTTGACTG6440637.150639125779984No Hit
TAGCAGCACGTAAATATTGGCG1978412.1965080982503853No Hit
GGAATACCGGGTGCTGTAGGCTTT1765291.9598939455221227No Hit
TTCAAGTAATCCAGGATAGGCT1757911.9517003811117688No Hit
AACATTCAACGCTGTCGGTGAGT1687691.8737393929146096No Hit
AAGCTGCCAGTTGAAGAACTGT1584641.7593292557212563No Hit
AACATTCAACGCTGTCGGTGAG1276721.4174644382095885No Hit
TAGCTTATCAGACTGATGTTGAC1094291.2149235228463333No Hit
GGAATACCGGGTGCTGTAGGCTT1048621.1642189040630198No Hit
TAGCTTATCAGACTGATGTTGACT889600.9876686855624176No Hit
GCCTGTCTGAGCGTCGCT851990.9459125937638537No Hit
TCGTACGACTCTTAGCGG832570.9243517508303757No Hit
TATTGCACTTGTCCCGGCCTGTAT784340.8708049200022782No Hit
TATTGCACTTGTCCCGGCCTGTAA759330.8430378406116351No Hit
TATTGCACTTGTCCCGGCCTGT695490.7721601777448357No Hit
TAGCTTATCAGACTGATGTTGA686730.7624344834040907No Hit
TGAGGTAGTAGGTTGTATAGTT672660.746813419548579No Hit
TGTAAACATCCTTGACTGGAAGCT525700.5836526843527012No Hit
TATTGCACTTGTCCCGGCCTGTA512700.5692195763127827No Hit
CATAAAGTAGAAAGCACTACT453130.5030826343175565No Hit
AGCAGCATTGTACAGGGCTATGA424540.471340899020536No Hit
TGAGGTAGTAGATTGTATAGTT413580.45917267870380474No Hit
TTCAAGTAATTCAGGATAGGTT402480.4468490249158744No Hit
AACCGGGCGGAAACACCA361590.401451348934931No Hit
CCCATAAAGTAGAAAGCACTAC352210.39103730636458983No Hit
CAACGGAATCCCAAAAGCAGCT351730.3905043916061929No Hit
ACTGGACTTGGAGTCAGAAGGC334600.3714859961659004No Hit
CAACGGAATCCCAAAAGCAGCTGT326600.3626040835259506No Hit
GGAATACCGGGTGCTGTAGGCTTA309940.3441075004532551No Hit
TGTAAACATCCCCGACTGGAAGCT302360.3356918882269027No Hit
TGAATACCGGGTGCTGTAGGCTTT285910.3174284553610059No Hit
TTCTCACTACTGCACTTGACTAG274900.30520472309027497No Hit
CCCATAAAGTAGAAAGCACT260560.28928389468316495No Hit
CATTGCACTTGTCTCGGTCTGA257530.28591987027078397No Hit
ACCCCCCACTGCTAAATTTGACTG253770.2817453713300076No Hit
CACCCGGCTGTGTGCACATGTGC243950.2708428235644692No Hit
GACCCGCGGGCGCTCTCCAGTCCT240510.2670236011292908No Hit
CCCCCCACTGCTAAATTTGACTGG228680.25388947281296503No Hit
CTTCTCACTACTGCACTTGACTAG213590.23713596509585969No Hit
CGCCTGTCTGAGCGTCGCT197880.2196941091491583No Hit
CTCCCACTGCTTCACTTGACTAG195320.21685189710437436No Hit
TCGTTTCCCGGCCAATGCACCA194110.21550850781758193No Hit
CCCCCCACTGCTAAATTTGACTGT174000.19318159991890815No Hit
TGTAAACATCCTACACTCTCAGCT172050.19101663371292038No Hit
AACGGAATCCCAAAAGCAGCTG171560.19047261656372344No Hit
TGAATACCGGGTGCTGTAGGCTT167010.185421028749752No Hit
TGAGGTAGTAGTTTGTACAGTT163740.1817905469581725No Hit
TAGCAGCACGTAAATATTGGC156670.1739411566626169No Hit
GGAATACCGGGTGCTGTAGGCT155370.17249784585862504No Hit
TAGCTTATCAGACTGATGTTGACA154510.17154304024983044No Hit
CAACACCAGTCGATGGGCTGT151630.1683455516994485No Hit
CCCCCACTGCTAAATTTGACTGGCTT149140.16558105639026413No Hit
ACCGGGTGCTGTAGGCTT146880.1630719160694783No Hit
TCCTGTCTGAGCGTCGCT145440.16147317179428736No Hit
CCCCCCACTGCTAAATTTGACTGTA143680.1595191510134984No Hit
CCCCCCACTGCTAAATTTGACTGGCT134870.14973794471875368No Hit
TATTGCACTTGTCCCGGCCTGTT131810.14634061313397287No Hit
CAAAGAATTCTCCTTTTGGGCT131660.14617407727197382No Hit
AACATTCAACGCTGTCGGTGAGTT115850.12862119741727304No Hit
TGAGGTAGTAGTTTGTGCTGTT114720.1273666272568801No Hit
ACCGGGTGCTGTAGGCTTA113760.12630079774008615No Hit
ACCGGGTGCTGTAGGCTTT113510.12602323797008771No Hit
CCCCCCACTGCTAAATTTGACTGA113230.12571237102768948No Hit
CACTAGATTGTGAGCTCCTGGA112200.12456882477529595No Hit
AGCTCGGTCTGAGGCCCCTCAGT107200.11901762937532731No Hit
TTCAAGTAATCCAGGATAGGCTT100280.11133477494177074No Hit
TATTGCACTTGTCCCGGCCTGTAG98300.10913650156338317No Hit
CCCCCACAACCGCGCTTGACTAG93520.10382955876101316No Hit
AGCAGCATTGTACAGGGCTATGT92110.10226412165822199No Hit
CACTAGATTGTGAGCTCCTGGAA90900.10092073237142961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)