FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003947590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003947590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7794830
Sequences flagged as poor quality0
Sequence length18-26
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCACTGCTAAATTTGACTG192063324.639831785940167No Hit
CAACGGAATCCCAAAAGCAGCTG5686317.294976285563637No Hit
CCCCCACTGCTAAATTTGACTG5399596.927142734350846No Hit
TTCAAGTAATCCAGGATAGGCT1952872.5053400779747603No Hit
TAGCAGCACGTAAATATTGGCG1747632.242037350397635No Hit
AACATTCAACGCTGTCGGTGAGT1737632.2292083342420552No Hit
AAGCTGCCAGTTGAAGAACTGT1681402.157070776399229No Hit
AACATTCAACGCTGTCGGTGAG1236471.5862693605890057No Hit
TATTGCACTTGTCCCGGCCTGTAT966941.2404888881476568No Hit
TATTGCACTTGTCCCGGCCTGTAA925951.1879027509259341No Hit
TAGCTTATCAGACTGATGTTGACT835401.0717360096371569No Hit
TAGCTTATCAGACTGATGTTGAC834941.0711458748940001No Hit
TATTGCACTTGTCCCGGCCTGT602010.7723196015820744No Hit
TAGCTTATCAGACTGATGTTGA575540.7383611958182539No Hit
TATTGCACTTGTCCCGGCCTGTA533060.6838635351893498No Hit
TGTAAACATCCTTGACTGGAAGCT528430.6779237007093163No Hit
TGAGGTAGTAGGTTGTATAGTT519500.6664673892823834No Hit
AGCAGCATTGTACAGGGCTATGA370940.47587952527508615No Hit
CCCATAAAGTAGAAAGCACTAC362660.46525709989826586No Hit
CATAAAGTAGAAAGCACTACT353370.453338943889732No Hit
TGAGGTAGTAGATTGTATAGTT327050.4195729733682454No Hit
TTCAAGTAATTCAGGATAGGTT316880.4065258639380205No Hit
TTCTCACTACTGCACTTGACTAG312700.401163335184988No Hit
CAACGGAATCCCAAAAGCAGCTGT305300.39166986322985875No Hit
ACCCCCCACTGCTAAATTTGACTG293890.3770319557963419No Hit
TGTAAACATCCCCGACTGGAAGCT276530.354760783750255No Hit
ACTGGACTTGGAGTCAGAAGGC274720.35243873182609503No Hit
CAACGGAATCCCAAAAGCAGCT274040.35156635872751557No Hit
TCGTACGACTCTTAGCGG257080.3298083473276518No Hit
CTTCTCACTACTGCACTTGACTAG252110.3234323262983285No Hit
CCCATAAAGTAGAAAGCACT219770.28194328805118263No Hit
CCCCCCACTGCTAAATTTGACTGT219290.2813274952757148No Hit
CCCCCCACTGCTAAATTTGACTGG204310.2621096290746559No Hit
CATTGCACTTGTCTCGGTCTGA201570.258594478648027No Hit
TGTAAACATCCTACACTCTCAGCT199190.25554117280299893No Hit
CACCCGGCTGTGTGCACATGTGC192290.2466891516556487No Hit
GACCCGCGGGCGCTCTCCAGTCCT189900.24362301679446505No Hit
CCCACCACTGCTAAATTTGACTG188710.24209636387195105No Hit
CCACCCACTGCTAAATTTGACTG185520.238003907718321No Hit
TCGTTTCCCGGCCAATGCACCA185490.23796542066985427No Hit
TTAATGCTAATCGTGATAGGGGTT185190.23758055018518687No Hit
CTCCCACTGCTTCACTTGACTAG183510.2354252754710494No Hit
CACCCCACTGCTAAATTTGACTG162320.2082405902373753No Hit
CAAAGAATTCTCCTTTTGGGCT155070.19893955352457976No Hit
CCCCCCACTGCTAAATTTGACTGTA150660.19328195739996895No Hit
CCCCCACTGCTAAATTTGACTGGCTT142770.1831598636532163No Hit
TGAGGTAGTAGTTTGTACAGTT134200.1721653968078842No Hit
CCCCCCACTGCTAAATTTGACTGA132330.16976637078679074No Hit
ACCCCCACTGCTAAATTTGACTG131830.16912491997901175No Hit
TATTGCACTTGTCCCGGCCTGTT127480.1635442979513344No Hit
TAGCTTATCAGACTGATGTTGACA121170.1554491887571634No Hit
AACGGAATCCCAAAAGCAGCTG120800.15497451515940694No Hit
TATTGCACTTGTCCCGGCCTGTAG117930.15129258752275548No Hit
AACATTCAACGCTGTCGGTGAGTT116760.14979159263255262No Hit
AGCTCGGTCTGAGGCCCCTCAGT114520.14691789301370267No Hit
CACTAGATTGTGAGCTCCTGGA112860.14478827633187638No Hit
CCCCCAACTGCTAAATTTGACTG110710.1420300378584267No Hit
CCCCCCACTGCTAAATTTGACTGGCT106860.13709086663852837No Hit
CACTAGATTGTGAGCTCCTGGAA103130.132305643612497No Hit
TAGCAGCACGTAAATATTGGC102880.1319849182086075No Hit
CCCCACACTGCTAAATTTGACTG101950.13079181970613857No Hit
TTCAAGTAATCCAGGATAGGCTT101400.13008622381758167No Hit
TCGTTTCCCGGGCGGCGCACCA98820.126776337649442No Hit
TGATGTAGTAGGTTGTATAGTT95890.12301743591585704No Hit
CCCCCACAACCGCGCTTGACTAG93830.12037465858780755No Hit
CAAAGAATTCTCCTTTTGGGCTT93290.11968189171540625No Hit
CAACACCAGTCGATGGGCTGT92080.11812958076058105No Hit
CGCCTGTCTGAGCGTCGCT91870.11786017142131387No Hit
GGAATACCGGGTGCTGTAGGCTTT88650.11372922821921709No Hit
CCCCCCACTGCTAAATTTTACTG86640.1111505959719455No Hit
TATTGCACTTGTCCCGGCCTGTTT85880.11017559074412142No Hit
TTCAAGTAATCCAGGATAGGC85860.11014993271181027No Hit
AGCAGCATTGTACAGGGCTATGT85240.1093545337101643No Hit
TGTAAACATCCCCGACTGGAAGC84830.10882854404778552No Hit
TGTCAGTTTGTCAAATACCCCA84490.1083923574984958No Hit
GCCCCCACTGCTAAATTTGACTG84390.10826406733694No Hit
CCCCCCACTGCTAAATTTGAC83820.10753281341607193No Hit
ACCGGGTGCTGTAGGCTTA83650.10731472014142707No Hit
TGAGGTAGTAGTTTGTGCTGTT81320.10432555937717693No Hit
ACCGGGTGCTGTAGGCTT80650.10346601529475306No Hit
CCCATAAAGTAGAAAGCACTA79410.10187521729146114No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)